Insight application of cytotoxicity assay of Turbinaria Ornata (Brown seaweed) with reference to anticancer properties
Paper Details
Insight application of cytotoxicity assay of Turbinaria Ornata (Brown seaweed) with reference to anticancer properties
Abstract
Turbinaria ornata is a species of marine brown seaweed and in the family Sargassaceae. They were freshly collected from Mandapam Coastal Area, Rameswaram Tamil Nadu, India and rinsed in seawater and packed in sterile bags for further proceedings to laboratory. Seaweeds have the potential to be utilized as renewable resources within the marine environment. An invitro study of Turbinaria ornata in cell lines were human HepG2 hepatic carcinoma cells, liver cancer cell line HepG2 preserved in RPMI-1640 medium at 37 °C Turbunaria ornata ethanolic extract (TOE) using cell viability assay and cytotoxic effect (Brown sea weed), The effect of TOE and standard anticancer drug Doxorubicin (DOX) (1µm/ml) on cell growth inhibition was assessed as percent cell viability where vehicle-treated cells were taken as 100% viable. The supernatant was removed and 50 µl of propanol was added and the plates were gently shaken to solubilize the formed formazan, MTT assay of yellow 3-(4, 5dimethythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) HepG2 cells and treated with various concentration of TOE (25-200 μg/ml) and standard doxorubicin (1µm) for 24h. The half maximal inhibitory concentration (IC50) of TOE as the percentage survival of the cells. The fluorescence microscopy images of the untreated HepG2 cells (A). HepG2 cells treated with TOE (100µg) (B), TOE (200µg) (C) and DOX (D) for 24 hours. In the DAIP Staining, the yellow arrows indicate intact nucleus, green arrows, mitotic cells and the red arrows show the apoptotic cells. TOE at 100 and 200 μg showed apoptosis on HepG2 cells, which may in part account for the anticancer activity.
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S. Parathasarathi, M. Jeyaraj (2024), Insight application of cytotoxicity assay of Turbinaria Ornata (Brown seaweed) with reference to anticancer properties; IJB, V25, N2, August, P180-188
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