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Interaction of chenodeoxycholic acid with cholesterol in a model system studied by spin label probe method

Sh. A. Kholova, Kh. Sh. Dzhuraev, I. Kh. Yusupov, G. I. Likhtenshtein

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Int. J. Biomol. & Biomed.4(1), 1-6, January 2014


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Interaction of cholesterol which is an essential structural component of animal cell membranes that is required to establish proper membrane permeability and fluidity and the bile chenodeoxycholic acid which significantly increased serum total cholesterol, and high-density-lipoprotein (HDL) cholesterol, and dissolves gallstones appears to be one of pivotal problem of biomedical gastroenterology. The methods of spin labels and spin probes have been proved to be power tools for solving structural and dynamical problems of chemistry and biology on molecular level. In present work the method of spin probes was used for investigation of quantitative interaction of cholesterol and chenodeoxycholic acid in ethanol possessing dielectric properties similar to ones of biological membranes. Using Electron Spin Resonance of nitroxide spin probe, it was shown that in the ethanol solution cholesterol molecules form aggregates, modeling a gallstone, which effectively bound chenodeoxycholic acid. The developed method can be used for study relationships between cholesterol and chenodeoxycholic acid in the in human blood, high-density-lipoproteins and gallstones.


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Interaction of chenodeoxycholic acid with cholesterol in a model system studied by spin label probe method

Alan R. and Gaby MD. 2009. Nutritional Approaches to Prevention and Treatment of Gallstones. Alternative Medicine Review. 14, 258-226

Beers MH, Porter RS, Jones TV, eds. 2006. Merck Manual of Diagnosis and Therapy (18th ed.). Whitehouse Station, New Jersey: Merck Sharp & Dohme Corporation.

Berliner L. (ed.) 1976 Spin Labeling. Theory and Applications, Vol. 1, Academic Press, New York.

Berliner L. (ed.) 1998. Spin Labeling. The Next Millennium Academic Press, New York.

Doty JE, DenBesten L, Roslyn JJ, Pitt HA, Kuchenbecker SL, Porter-Fink V. 1982. Interaction of chenodeoxycholic acid and dietary cholesterol in the treatment of cholesterol gallstones. American Journal of Surgery 143, 48-54.

 Freed JH. 1976. Theory of the ESR spectra of nitroxids in Spin Labeling. Theory and Applications,Berliner, L( ed.) Vol.1, Academic Press, New York.

Fromm H. 1989. Bile acid-lipoprotein interactions: effects of ursodeoxycholic acid (ursodiol). Digestive Diseases and Science 34, S21-S23 .

Griffith ON and Jost P.C. 1976. Spin labeled membranes. In Spin labeling. Theory and Application. ‘ Berliner L (ed) Vol. 1Academic Press, New York, pp. 488-569

 Incardona JP, Eaton S. 2000. Cholesterol in signal transduction. Current Opinion .of Cell Biology 12, 193– 203.

 Iser JH and Sali A. 1981. Chenodeoxycholic acid: a review of its pharmacological properties and therapeutic use. Drugs. 21, 90-119.

Kivelson D. 1960. Theory of EPR [electron paramagnetic resonance] line widths of free radicals. Journal Chemical Physics 33, 1094-1106 .

Likhtenshtein G.I. 1976. Spin Labeling Method in Molecular  Biology,  N.  Y.,  Wiley  Interscience,  New York.

Likhtenshtein GI. 1993. Biophysical Labeling Methods in Molecular Biology,.Cambridge, New York,.Cambridge University Press.

Likhtenshtein G.I. 2000. Labeling, Biophysical, Encyclopedia of Molecular Biology and Molecular Medecine, Vol. 7, Meyers, R. (ed.), VCH, New-York, pp. 157-138.

Likhtenshtein GI, Yamauchi J, Nakatsuji S, Smirnov A., Tamura R. 2008. Nitroxides: Application in Chemistry, Biomedicine, and Materials Science. WILEY-VCH, Weinhem.

Ohvo-Rekilä H, Ramstedt B, Leppimäki P, Slotte JP. 2002. Cholesterol interactions with phospholipids in membranes. Progress of Lipid Research 41, 66–97.

Petroni ML, Jazrawi RP, Pazzi P, Lanzini A, Zuin M, Pigozzi MG, Fracchia M, Galatola G, Alvisi V, Heaton KW, Podda M, Northfield TC. 2001. Ursodeoxycholic acid alone or with chenodeoxycholic acid for dissolution of cholesterol gallstones: a randomized multicentre trial. The British-Italian Gallstone Study group. Aliment Pharmacology Therapy 15, 123-128.

Rothblat GH, Mahlberg FH, Johnson WJ, Phillips MC. 1992. Apolipoproteins, membrane cholesterol domains, and the regulation of cholesterol efflux. The Journal of Lipid Research 33, 1091-1097.


Schroeder F, Jefferson JR, Kier AB, Knittel J, Scallen TJ, Wood WG, Hapala I. 1991. Membrane cholesterol dynamics: cholesterol domains and kinetic pools. Proceedings of The Society for Experimental Biology and Medicine Experimental Biology and Medicine 196, 235-252.

 van der Steeg WA, Holme I, Boekholdt SM, Larsen ML, Lindahl C, Stroes ES, Tikkanen MJ, Wareham NJ, Faergeman O, Olsson AG, Pedersen TR, Khaw KT. 2008. High-density lipoprotein cholesterol, high-density lipoprotein particle size, and apolipoprotein A-I: significance for cardiovascular risk: the IDEAL and EPIC-Norfolk studies. Journal of the American College of Cardiology 51.(6): 634–642.

 Wolkoff AW and Cohen DE. 2003. Bile acid regulation of hepatic physiology: I. Hepatocyte transport of bile acids. American Journal of Physiology 284, G175–179.


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