J. Bio. Env. Sci.5(1), 189-198, July 2014
During the growing season, young raspberry leaf samples were collected from natural populations in northern Iran. A total of 65 Rubus samples were collected at altitudes ranging from 0 to 1021 meter above sea level. Morphometric and molecular studies were performed in seven Rubus species and four uncertain species from the sect. Rubus L. 7 species including R. caecius, R. sanctus, R, discolor, R. dolichocarpus, R. hyrcanus, R. persicus and R. hirtus are. In total, the delimitation of species of Rubus northern Iran, from 27 morphological characters and 13 SSR primer pairs were used. The dendrogram based on quantitative morphological traits, species are completely separated, and even species were differentiated on the basis of geographical pattern. A total of 12 loci and 29 alleles were detected. The number of alleles observed for each locus ranged from 1 to 6, with an average of 3.08 alleles per locus. The SSRs achieved cumulative polymorphism information content (PIC) of 0.9444. These PIC values demonstrated that, in spite of the low number of primers used, the SSR were sufficiently polymorphic and informative. Expected heterozygosities (He) were 0.021 to 0.286. The dendrogram based on microsatellite markers, no clear geographic pattern to interpret the results, we do not see. Status is unknown species and genotypes with high affinity and proximity to each other show. In general, the limits of species, the occurrence of hybridization between species with different ploidy levels between Rubus genotypes, is not subject to any specific geographic pattern.
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