Investigation in qualitative characteristics of breed wheat cultivars using biochemical-molecular markers and micronutrients
Paper Details
Investigation in qualitative characteristics of breed wheat cultivars using biochemical-molecular markers and micronutrients
Abstract
This experiment was aimed to investigate the genetic diversity of high and low molecular weight glutenin subunits along with Fe and Zn concentrations in grains of 17 wheat cultivars/lines. For this, Total grain protein was extracted and electrophoresis of HMW-GS was carried out using SDS-PAGE on poly acrylamide gels. Specific primers were selected for LMW-GS and glidine subunits and PCR products were separated on agarose gels. Grain samples were analyzed for zinc and iron using atomic absorption spectroscopy. The most common composition of HMW-GS subunits was (null, 7+8, 2+12). Also, compositions (2*, 7+8, 5+10), (1, 7+8/6+8, 5+10) and (null, 17+18, 5+10) that give the maximum quality grade 10, 10/8 and 8 to the genotypes possessing them, were observed in Sasyon, Gaspard, and LineA, respectively. Maximum LMW-GS and gliadin alleles number, 11, 10, 10, 10, 9, 9, 9, that give highly potential quality to the genotypes possessing them, were observed in Niknejad, Sasyon, Shahpasand, Bayat, Shahriyar, Sorkhtokhm and Darya, respectively. Among studied genotypes, the concentrations of grain Fe varied by 1.80 fold, ranging from 25.28 (Sorkhtokhm) to 45.62 (LineA) µg.g-1, and grain Zn varied by 2.49 fold, from 28.85 (Sepahan) to 72.04 (Alamut) µg.g-1. Also, highly positive correlation was observed between grains Fe with Zn. In conclusion Niknejad, Sasyon and LineA with high potential of the bakery property and iron and zinc contents altogether were introduced as appropriate candidate parents in future wheat breeding programs.
Ahmadi J, Pour-Aboughadareh A. 2015. Allelic variation of glutenin and gliadin genes in Iranian einkorn wheat. Journal of Biodiversity and Environmental Sciences 7, 168–179.
Aguiriano E, Ruiz M, Fite R, Carrillo JM. 2006. Analysis of genetic variability in a sample of the durum wheat (Triticum durum) Spanish collection based on gliadin markers. Genetic Resources and Crop Evolution 53, 1543–1552.
An X, Zhang Q, Yan Y, Li Q, Zhang Y, Wang A, Pei Y, Tian J, Wang H, Hsam SLK, Zeller FJ. 2006. Cloning and molecular characterization of three novel LMW-i glutenin subunit genes from cultivated einkorn (Triticum monococcum L.). Theoretical and Applied Genetics 113, 383–395.
Anderson OD, Yong QG, Xiuying K, Gerard RL and Jiajie W. 2009. The wheat ω-gliadin genes: structure and EST analysis. Functional & Integrative Genomics 9(3), 397–410. http://dx.doi.org/10.1007/s10142-009-0122-2.
Badakhshan H, Moradi N, Mohammadzadeh H, Zakeri MR. 2013. Genetic variability analysis of grains Fe, Zn and beta-carotene concentration of prevalent wheat varieties in Iran. International journal of agriculture and crop sciences 6(2), 57.
Bahraei S, Saidi A, Alizadeh D. 2004. High molecular weight glutenin subunits of current bread wheats grown in Iran. Euphytica 137, 173–179.
Benmoussa M, Vezina LP, Page M, Yelle S, Laberge S. 2000. Genetic polymorphism in low-molecular weight glutenin genes from Triticum aestivum, variety Chinese Spring. Theoretical and Applied Genetics 100, 789-793.
Caballero L, Martin MA, Alvarez JB. 2008. Allelic variation for the high- and low-molecular-weight glutenin subunits in wild diploid wheat (Triticum urartu) and its comparison with durum wheats. Australian Journal of Agricultural Research 59, 906–910.
Cakmak I. 2008. Enrichment of cereal grains with zinc: agronomic or genetic biofortification. Plant Soil 302, 1-17. http://dx.doi.org/10.1007/s11104-007-9466-3
Cakmak I, Tourn A, Millet E, Feldman M, Fahima T, Korol A, Nevo E, Braun HJ, Ozkan H. 2004. Triticumdicoccoides: an important genetic resource for increasing zinc and iron concentration in modern cultivated wheat. Soil Science and Plant Nutrition 50(7), 1047-1054.
Cakmak I, Pfeiffer WH, McClafferty B. 2010.Biofortification of durum wheat with zinc and iron. Cereal Chemistry 87, 10–20. https://doi.org/10.1094/CCHEM-87-1-0010.
Chatzav M, Peleg Z, Ozturk L, Yazici A, Fahima T, Cakmak I, Saranga Y. 2010. Genetic diversity of grain nutrients in wild emmer wheat: potential for wheat improvement. Annals of Botany 105 (7), 1211-1220. https://doi.org/10.1093/aob/mcq024.
Cornish GB, Békés F, Eagles HA, Payne PI. 2006. Prediction of dough properties for bread wheats. In: Wrigley CW, Bekes F, Bushuk W (eds), Gliadin and Glutenin. The Unique Balance of Wheat Quality. AACCI Press. St Paul, Min., USA. 243–280 P.
Cuesta S, Alvarez JB, Guzman C. 2017. Identification and molecular characterization of novel LMW-m and –s glutenin genes, and a chimeric –m/-I glutenin gene in 1A chromosome of three diploid Triticum species. Journal of Cereal Science 74, 46–55.
Demirkiran AR. 2009. Determination of Fe, Cu and Zn contents of wheat and corn grains from different growing site. Journal of Animal and Veterinary Advances 8(8), 1563-1567.
Doyle JJ, Doyle JL. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19, 11–15.
Ficco DBM, Riefolo C, Nicastro G, De Simone V, Di Gesu AM, Beleggia R, Platani C, Cattivelli L, De Vita P. 2009. Phytate and mineral elements concentration in a collection of Italian durum wheat cultivars. Field Crops Resarch 111(3), 235–242. http://dx.doi.org/10.1016/j.fcr.2008.12.010.
Figueroa JDC, Maucher T, Reule W, Peña RJ. 2009. Influence of high molecular weight glutenins on viscoelastic properties of intact wheat kernel and relation to functional properties of wheat dough. Cereal Chemestry 86,139–144. http://dx.doi.org/10.1094/CCHEM-86-2-0139.
Garcia-Oliveira AL, Tan L, Fu Y, Sun C. 2009. Genetic identification of quantitative trait loci for contents of mineral nutrients in rice grain. Journal of Integrative Plant Biology 51(1), 84-92.
Graham RD, Welch RM, Bouis HE. 2001. Addressing micronutrient malnutrition through enhancing the nutritional quality of staple foods: principles, perspectives and knowledge gaps. Advances in Agronomy 70, 77-142.
Gelin JR, Forster S, Grafton KF, McClean PE, Rojas-Cifuentes GA. 2007. Analysis of seed zinc and other minerals in a recombinant inbred population of navy bean (Phaseolus vulgaris L.). Crop Science 47, 1361-1366.
Genc Y, Humphries JM, Lyons GH, Graham RD. 2005. Exploiting genotypic variation in plant nutrient accumulation to alleviate micronutrient deficiency in populations. Journal of Trace Elements in Medicine and Biology 18, 319-324.
Gomez-Becerra HF, Erdem H, Yazici A, Tutus Y, Torun B, Ozturk L, Cakmak I. 2010a. Grain concentrations of protein and mineral nutrients in a large collection of spelt wheat grown under different environments. Journal of Cereal Science 52, 342-349. http://dx.doi.org/10.1016/j.jcs.2010.05.003.
Gomez-Becerra HF, Yazici A, Ozturk L, Budak H, Peleg Z, Morgounov A, Fahima T, Saranga Y, Cakmak I. 2010b. Genetic variation and environmental stability of grain mineral nutrient concentrations in Triticum dicoccoides under five environments. Euphytica 171, 39-52. http://dx.doi.org/10.1007/s10681-009-9987-3.
Gupta RB, Shepherd KW. 1990. Two-step one dimensional SDS-PAGE analysis of LMW subunits of glutenin. I. Variation and genetic control of the subunits in hexaploid wheats. Theoretical and Applied Genetics 80, 65–74.
Gupta RB, Singh NK and Shepherd KW. 1989. The cumulative effect of allelic variation in LMW and HMW glutenin subunits on physical dough properties in progeny of two bread wheats. Theoretical and Applied Genetics 77, 57-64.
Gupta PK. 2000. Soil, plant, water and fertilizer analysis. Agrobios, New Dehli, India. P. 438.
Harmankaya M, Ozcan MM, Gezgin S. 2012. Variation of heavy metal and micro and macro element concentrations of bread and durum wheats and their relationship in grain of Turkish wheat cultivars. Environmental Monitoring and Assessment 184(9), 5511–5521. http://dx.doi.org/10.1007/s10661-011-2357-3.
Ikeda TM, Branlard G, Peña RJ, Takata K, Liu L, He Z, Lerner SE, Kolman MA, Yoshida H, Rogers WJ. 2008. International collaboration for unifying Glu-3nomenclature system in common wheats. In: International Wheat Genetics Symposium, Brisbane, Australia. http://ses.library.usyd.edu.au/bitstream/2123/3281/1/O42.pdf
Ikeda TM, Nagamine T, Fukuoka H, Yano H. 2002. Identification of new low-molecular-weight glutenin subunit genes in wheat. Theoretical and Applied Genetics 104, 680-687.
Igrejas G, Guedes-Pinto H, Cardine V, Branlard G. 1999.The high and low molecular weight glutenin subunits and omega gliadin composition of bread and durum wheats commonly grown in Portugal. Plant Breeding 118, 297–302.
Kawaura K, Mochida K, Ogihara Y. 2005. Expression profile of two storage-protein gene families in hexaploid wheat revealed by large-scale analysis of expressed sequence tags. Plant Physiology 139, 1870–1880.
Masoudi-Nejad A, Nasuda S, Kawabe A, Endo TR. 2002. Molecular cloning, sequencing, and chromosome mapping of a 1A-encoded ω-type prolamin sequence from wheat. Genome 45, 661–669.
Moghaddam M, Ehdaie B, Waines JG. 2000. Genetic diversity in population of wild diploid wheat Triticum urartu revealed by isozyme markers. Genetic Resources and Crop Evolution 47, 323–334.
Morgunov AI, Pena RJ, Crossa J, Rajam S. 1993. Worldwide distribution of Glu-1 alleles in bread wheat. Journal of Genetics and Breeding 47, 53-60.
Morgounov A, Gomez-Becerra HF, Abugalieva A, Dzhunusova M, Yessimbekova M, Muminjanov H, Zelenskiy Y, Ozturk L, Cakmak I. 2007. Iron and zinc grain diversity in common wheat grown in central Asia. Euphytica 155, 193-203.
Nakamura H. 2001. Genetic diversity of high molecular-weight glutenin subunits compositions of in landraces of hexaploid wheat from Japan. Euphytica 120, 227-234.
Liu L, He ZH, Yan J, Zhang Y, Xia X, Pena RJ. 2005. Allelic variation at the Glu-1and Glu-3loci, presence of the 1B.1R translocation, and their mixographic properties in Chinese bread wheats. Euphytica 142,197–204.
Long H, Wei YM, Yan ZH, Baun B. 2005. Classification of wheat low molecular weight glutenin subunit genes and its chromosome assignment by development LMW-GS group specific primers. Theoretical and Applied Genetics 111, 1251-1259.
Lukow OM. 1991. Screening of bread wheats formilling and baking quality- A Canadian perspective. Cereal Foods World 36(6), 497-501.
Luo C, Griffin WB, Branlard G, McNeil DL. 2001. Comparison of low and high molecular weight wheat glutenin allele effects on flour quality. Theoretical and Applied Genetics 102, 1088–1098.
Ortiz-Monasterio JI, Palacios-Rojas E, Meng E, Pixley K, Trethowan R, Pena RJ. 2007. Enhancing the mineral and vitamin content of wheat and maize through plant breeding. Journal of Cereal Science 46, 293- 307. http://dx.doi.org/10.1016/j.
Oury FX, Leenhardt F, Remesy C, Chanliaud E, Duperrier B, Balfourier F, Charmet G. 2006. Genetic variability and stability of grain magnesium, zinc and iron concentrations in bread wheat. European Journal of Agronomy 25, 177-185. http://dx.doi.org/10.1016/j.eja.2006.04.011.
Payne PI, Corfield KG, Blackman JA. 1981. Correlation between the inheritance of certain high-molecular-weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat. Journal of the Science of Food and Agriculture 32, 51–60.
Payne PI, Lawrence GJ. 1983. Catalogue of alleles or the complex loci, Glu-A1, Glu-B1and Glu-D1which coded for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Research Communications 11, 29–35.
Payne IP, Jackson EA, Holt LM, Law CN.1984. Genetic linkage between endosperm storage protein genes on each of the short arms of chromosomes 1A and 1B in wheat. Theoretical and Applied Genetics 67, 235–243.
Payne PI, Mark AN, Krattiger AF, Holt LM. 1987. The relationship between HMW glutenin subunits composition and the bread making quality of British-grown wheats. Journal of the Science of Food and Agriculture 40, 51-65.
Payne PI. 1987. Genetic of wheat storage proteins and the effect of allelic variation on bread making quality. Annual Reviews in Plant Physiology 8, 141-153.
Peleg Z, Saranga Y, Yazici A, Fahima T, Ozturk L, Cakmak I. 2008. Grain zinc, iron and protein concentrations and zinc-efficiency in wild emmer wheat under contrasting irrigation regimes. Plant Soil 306(1–2), 57–67. http://dx.doi.org/10.1007/s11104-007-9417-z.
Peleg Z, Cakmack I, Ozturk L, Yazici A, Budak H, Korol AB, Fahima T, Saranga Y. 2009. Quantitative trait loci conferring grain mineral nutrient concentrations in durum wheat × wild emmer wheat RIL population. Theoretical and Applied Genetics 119, 353-369. http://dx.doi.org/10.1007/s00122-009-1044-z.
Rawat N, Tiwari VK, Singh N, Randhawa GS, Singh K, Chhuneja P, Dhaliwal HS. 2009. Evaluation and utilization of Aegilops and wild Triticum species for enhancing iron and zinc content in wheat. Genetic Resources and Crop Evolution 56(1), 53–64.
Saltzman A, Ekin B, Howarth EB, Erick B, Fabiana FDM, Yassir I, Wolfgang HP. 2013. Biofortification: Progress toward a more nourishing future. Global Food Security 2, 9-17. http://dx.doi.org/10.1016/j.gfs.2012.12.003.
Shewry PR, Tatham AS. 1997. Disulphide bonds in wheat gluten proteins. Journal of Cereal Science 25, 207-227.
Shewry PR, TathamAS, Barro F, Barcelo P, Lazzeri P. 1995. Biotechnology of unravelling and manipulating the multi-protein gluten complex. Biotechnology 13, 1185–1190.
Singh NK, Shepherd KW. 1988. Linkage mapping of genes controlling endosperm storage proteins in wheat I. Genes on the short arms of group 1 chromosomes. Theoretical and Applied Genetics 75, 628–641.
Stein AJ. 1994. Global impacts of human mineral malnutrition. Plant and Soil 335, 133-154.
Tahir M, Lafiandra D. 1994. Assessment of genetic variability in hexaploid wheat landraces of Pakistan based on polymorphism for HMW-glutenin subunits. In: Biochemical Evaluation of Plant Fenetic Resources, Final Technical Report, Dept. of Agrobiology and Agrobiochemistry, University of Tuscia, Viterbo, Italy, 33-44 P.
Tanaka H, Toyoda S, Tsujimoto H. 2005. Diversity of low-molecular-weight glutenin subunit genes in asian common wheat. Breeding Science 55, 349-354.
Velu G, Ortiz-Monasterio I, Singh R, Payne T. 2011. Variation for grain micronutrients concentration in wheat core-collection accessions of diverse origin. Asian Journal of Crop Science 3, 43–48.
Von Buren M. 2001. Polymorphisms in two homeologous γ-gliadin genes and the evolution of cultivated wheat. Genetic Resources and Crop Evolution 48, 205–220.
Wang S, Yin L, Tanaka H, Tanaka K, Tsujimoto H. 2011. Wheat- Aegilops chromosome addition lines showing high iron and zinc contents in grains. Breeding Science 61, 189-195.
Weegels PL, Van de Pijpekamp AM, Graveland A, Hamer RJ, Schofield JD. 1996. Depolymerisation and re-polymerisation of wheat glutenin during dough processing. I. Relationships between glutenin macropolymer content and quality parameters. Journal of Cereal Science 23,103–11.
Zhao FJ, Su YH, Dunham SJ, Rakszegi M, Bedo Z, McGrath SP, Shewry PR. 2009. Variation in mineral micronutrient concentrations in grain of wheat lines of diverse origin. J Cereal Sci 49 (2):290-295. http://dx.doi.org/10.1016/j.jcs.2008.11.007.
Zhao H, Guo B, Wei Y, Zhang B, Sun S, Zhang L, Yan J. 2011. Determining the geographic origin of wheat using multielement analysis and multivariate statistics. Journal of Agricultural and Food Chemistry 59(9), 4397–4402. http://dx.doi.org/10.1021/jf200108d.
Jafar Ahmadi (2017), Investigation in qualitative characteristics of breed wheat cultivars using biochemical-molecular markers and micronutrients; JBES, V11, N5, November, P149-160
https://innspub.net/investigation-in-qualitative-characteristics-of-breed-wheat-cultivars-using-biochemical-molecular-markers-and-micronutrients/
Copyright © 2017
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0