Investigation of 5-aminolevolinic acid (ALA) effects on seed germination and seedling growth of Silybum marianum under salinity stress

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Research Paper 01/09/2013
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Investigation of 5-aminolevolinic acid (ALA) effects on seed germination and seedling growth of Silybum marianum under salinity stress

Fardin Ghanbari, Batul Zarei, Alireza Pour-Aboughadareh, Sajad Kordi
Int. J. Biosci.3( 9), 95-101, September 2013.
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Salinity is a major environmental stress which adversely affects germination and seedling establishment in a wide variety of crops. However, studying of seeds with different plant growth regulators may helpful to alleviate the deleterious effects of salinity and enhance germination and seedling growth in salt stress environment. In present experiment we investigated the effects of 5-Aminolevolinic Acid (ALA) on germination and seedling growth parameters of milk thistle grown at different salinity levels. The experiment was arranged in factorial experiment in randomized complete block design with three replications. Results revealed that all parameters were significantly affected by salinity conditions and concentrations of ALA. In general, all parameters decreased with increasing NaCl concentrations, however the amount of these parameters increased with increasing ALA concentration. In addition, different between 0.25 and 0.50mM was not significant for germination percentage, shoot length, seedling fresh weight, seedling dry weight and leaf length. Interaction effects showed that highest value of root length and leaf length were produced from the 0.25 and 0.50mM ALA under the control treatment of salinity level. In contrast, the highest amount of seedling fresh weight were obtained from 0.25 and 0.50mM concentrations of ALA under control treatment of salinity. In general, our results suggest that ALA at low concentrations (0.25-1mM) has the potential to improve salt tolerance in milk thistle seedlings through concentrations treatment. We hope ALA may be useful in helping to solve serious problems occurring on a global scale, such as the desertification of green lands and salt damage to farm lands.


Aidid SB, Okamoto H. 1993. Responses of elongation growth rate, turgor pressure and cell wall extensibility of stem cells of Impatiens balsamina to lead, cadmium and zinc. Biometals 6, 245–249.

Agakishiev D. 1964. Effect of gibberellin on cotton under conditions of salinization. Fiziologiya Rastenii 11, 201-205, (not available DOI number)

Akhtar S, Wahid A, Akram M, Rasul E. 2001. Effect of NaCl salinity on yield parameters of some sugarcane genotypes. International Journal of Agriculture and Biology 3, 507-509.

Aldesuquy HS, Ibrahim AH. 2001.  Interactive effect of seawater and growth bio-regulators on water relations,  absicisic  acid  concentration  and  yield  of wheat plants. Journal of Agronomy and Crop Science 187, 185-193.

Ashraf M,  Foolad  MR.  2005.  Pre-sowing  seed treatment-A shotgun approach to improve germination, plant growth and crop yield under saline and  non-saline  conditions.  Advances  in  Agronomy 88, 223-265.

Balki AS, Padole. 1982. Effect of pre-soaking seed treatments with plant hormones on wheat under conditions of soil salinity. Journal of the Indian Society of Soil Science 30, 361-365.

Burgess CA. 2003. Silybum marianum (milk thistle), J Pharm Soc Winconsin. Mar/Apr, pp: 3-40.

Caasilit M, Whitecross MI, Nayudu M, Tanner GJ. 1997. UV-B irradiation induce differential leaf damage, ultrastructural changes and accumulation of specific phenolic compounds in rice cultivars. Australian Journal of Plant Physiology 24, 261–274.

Castelfranco PA, Rich PM, Beale SI. 1974. The abolition of the lag phase in greening cucumber cotyledons by exogenousd-aminolevulinic acid. Plant Physiology 53, 615–618.

Chakrabarti N, Mukherji S. 2003. Effect of Phytohormone pretreatment on nitrogen metabolism in Vigna radiateunder salt stress. Biologica Plantantrum 46, 63-66.

Das Gupta P, Das D, Mukherji S. 1994. Role of phytohormones in the reversal of stress-induced alteration in growth turgidity and proline accumulation in mungbean (Vigna radiate L. Wilczek) plants. Indian Biology 26, 343-348.

Daud MK, Variath MT, Ali S, Najeeb U, Muhammad J, Hayat Y, Dawood M, Khan MI, Zaffar M, Sardar AC, Tong XH, Zhu S. 2009. Cadmium-induced ultramorphological and physiological changes in leaves of two transgenic cotton cultivars and their wild relative. Journal of Hazardous Materials 168, 614–625.

Ellis RH, Roberts EH. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology 9, 373-409.

Ghavami N, Ramin AA. 2007. Salinity and temperature effect on seed germination of milk thistle. Communications in Soil Science and Plant Analysis 38 (19/20), 2681-2691.

Gilbert GA, Gadush MV, Wilson GC, Madore MA. 1998.  Amino  acid  accumulation  in  sink  and source tissues of Coleus Blumei Benth. During salinity stress,             Journal of Experimental Botany 49(318), 107-114.

Gulzar S, Ajmalkhan. 2001. Seed germination of a halophyte grass Aeluropus lagopoides. Annals of Botany 87, 319-324.

Hoque M, Haque S. 2002. Effects of GA3 and its mode of application on morphology and yield parameters of mungbean (Vigna radiate L.). Pakistan Journal of Biology Sciences 5, 281-283.

Hotta Y, Tanaka T, Bingshan L, Takeuchi Y and Konnai M. 1998. Improvement of cold resistance in rice seedlings by 5-aminolevulinic acid. Pesticide Science 23, 29–33,

Hotta Y, Tanaka T, Takaoka H, Takeuchi Y. 1997a. New physiological effects of 5-aminolevulinic acid in plants: the increase of photosynthesis, chlorophyll content, and plant growth. Bioscience, Biotechnology, and Biochemistry 61, 2025–2028,

Hotta YT, Tanaka Takaoka H, Takeuchi Y, Konnai M. 1997b. Promotive effects of 5-aminolevulinic acid on the yield of several crops. Plant Growth Regulation 22, 109–114,

Kandil AA, Sharief AE, Eloka MA. 2012a. Germination and Seedling Characters of Different Wheat Cultivars under Salinity Stress. Journal of Basic and Applied Sciences 8, 585-596.

Kandil AA, Sharief AE, Nassar EE. 2012b. Response of some rice (oryza sativa L.) cultivars to germination under salinity stress. International Journal of Agriculture Sciences. 4(6), 272-277.

Sedghi M, Nemati A. 2011. Influence of Different Priming Materials on Germination and Seedling Establishment of Milk Thistle (Silybum marianum) under Salinity Stress. World Applied Science Journal 11(5), 604-609.

Wahid A, Parveen M, Gelani S, Basra SMA. 2006. Pretreatment of seeds with H2O2 improves salt tolerance of wheat seedling by alleviation of oxidative damage and expression of stress proteins. Journal of Plant Physiology 164(3), 283-294.

Watanabe K, Tanaka T, Kuramochi H, Takeuchi Y. 2000. Improving salt tolerance of cotton seedling with 5-aminolevulinic acid. Plant Growth Regulation 32, 97–101.

Zhang WF, Zhang F, Raziuddin R, Gong HJ, Yang ZM, Lu L, Ye QF, Zhou WJ. 2008. Effects of  5-aminolevulinic  acid  on  oilseed  rape  seedling growth under herbicide toxicity stress. J Plant Growth Regulation 27, 159–169

Zhang ZJ, Li HZ, Zhou WJ, Takeuchi Y, Yoneyama K. 2006. Effect of 5-aminolevulinic acid on development and salt tolerance of potato (Solanum tuberosum L.) microtubers in vitro. Plant Growth Regulation 49, 27–34