Investigation of genetic diversity of some durum and bread wheat genotypes using SSR markers

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Research Paper 01/03/2015
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Investigation of genetic diversity of some durum and bread wheat genotypes using SSR markers

Reza Mir Drikvand, Goodarz Najafian, Elham, Aram Salahvarzi
J. Bio. Env. Sci.6( 3), 24-32, March 2015.
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Diversity is very important for breeding objective, since a narrow genetic base of germplasm is very vulnerable to biotic and abiotic stress. Genetic diversity of 40 wheat genotypes was assessed using 30 SSR primers that all of them were generated scorable bands. Totally 71 alleles (ranged between 2 to 4 alleles per each locus) was distinguished. Polymorphic information content (PIC) for all SSR primers was calculated. The highest (0.77) and the lowest (0.13) value of PIC was pertained to Xbarc352 and Xcfd56 Primers, respectively. According to similarity matrix, genetic similarity value ranged from 0.18 to 0.95 with an average of 0.48. The lowest and highest genetic similarity was observed between the Sistan and Arg (Bread wheat, No 27 and 28), Karkheh and Behrang (Durum wheat, No 35 and 38) genotypes respectively. Unweighted pair group method of the arithmetic average (UPGMA), based on Jaccard similarity clustering form a dendrogram with three genotypes group. Clustering somewhat was distinguished durum and bread wheat’s. Principle co-ordinate Analysis (PCA), 2D plot was confirmed the results of cluster analysis. Cophenetic correlation showed that molecular data and cluster was corresponded. It was concluded that SSR marker was suitable for evaluated of genetic diversity in wheat genotypes and this genetic diversity can be used in wheat breeding programs.


Akkaya MS, Buyukunal-Ba EB. 2003. Assessment of genetic variation of bread wheat varieties using microsatellite markers. Euphytica 135, 179–185.

Anderson JA, Churchill GA, Autrique JE, Tanksley SD, Sorrells ME. 1993. Optimization parental selection for genetic linkage maps. Genome 36, 181-186.

Botstein D, White RL, Skolnick M, Davis RW. 1980. Construction of a Genetic Linkage Map in Man Using Restriction Fragment Length Polymorphisms. The American Journal of Human Genetics 32, 314-331. PMid:6247908, PMCid:1686077.

Bryan GJ, Collins AJ, Stephenson P, Orry A, Smith JB, Gale MD. 1997. Isolation and characterization of microsatellites from hexaploid bread wheat. Theoretical and Applied Genetics 94, 557-563.

Chen HB, Martin JM, Lavin M, Talbert LE. 1994.  Genetic  diversity  in  hard  red  spring  wheat based on sequence tagged site PCR markers. Crop Science 34, 1629-1634.

Drikvand R, Bihamta MR, Najafian G, Ebrahimi A. 2013. Investigation of genetic diversity among bread wheat cultivars (Triticum aestivum L.) using SSR markers. Journal of Agriculture Science 5, 122-129. doi:10.5539/jas.v5n1p122

Fahima T, Roder M, Grama A, Nevo E. 1998. Microsatellite DNA polymorphism divergence in Triticum dicoccoides accessions highly resistant to yellow rust. Theoretical and Applied Genetics 96, 187-195.

Fu YB, Peterson GW, Richards KW, Somers D, DePauw RM, Clarke JM. 2005. Allelic reduction and genetic shift in the Canadian hard red spring wheat germplasm released from 1845 to 2004. Theoretical and Applied Genetics 110, 1505–1516.

Ismaeli A, Nazarian F, Samiei K, Drikvand R. 2010. Evaluation of genetic diversity among rainfed wheat genotypes using semi-random ISJ molecular marker Final research report Lorestan university, (In Persian).

Jaccard P. 1908. Nouvelles recherches sur la distribution florale. Bulletin Society Vaud Science National 44, 223-270.

Jamshidi S. 2011. NTSYSpc 2.02, implementation in molecular biodata analysis (clustering, screening, and individual selection). Proceedings of 4th International Conference on Environmental and Computer Science. Singapore, 16-18 September, 2011, pp. 165-169.

Landjeval S, Korzon V, Ganeva G. 2006. Evaluation of genetic diversity among Bulgarian winter wheat (Triticum aestivum L.) varieties during the period 1925-2003 using microsatellites. Genetic Resources and Crop Evolution 53, 1605-1614.

Maccaferri M, Sanguineti MC, Donini P, Tuberosa R. 2003. Microsatellite analysis reveals a progressive widening of the genetic basis in the elite durum  wheat  germplasm.  Theoretical  and  Applied Genetics 107, 783-797.

Naghavi MR, Mardi M, Ramshini HA, Fazelinasab B. 2004. Comparative analyses of the genetic diversity among bread wheat genotypes based on RAPD and SSR. Journal of Biotechnology 2, 195-202.

Parker GD, Fox PN, Langridge P, Chalmers K, Whan B, Ganter PF. 2002. Genetic diversity within Australian Wheat breeding programs based on molecular and pedigree data. Euphytica 124, 293– 306.

Powell W, Orozco-Castillo C, Chalmers KJ, Provan J, Waugh R. 1995. Polymerase chain reaction-based assays for the characterisation of plant genetic resources. Electrophoresis 16, 1726-30.

Prasad M, Varshney RK, Roy JK, Balyan HS, Gupta  PK.  2000.  The  use  of  microsatellites  for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theoretical and Applied Genetics 100, 584–592.

Roder MS, Korsun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal, MW. 1998. A microsatellite map of wheat. Genetics 149, 2007-2023.

Roder MS, Plaschke J, Konig SU, Borner A, Sorrells ME, Tanksley SD. 1995. Abundance variability and chromosomal location of microsatellite in wheat. Molecular Genetics and Genomics 246, 327–333.

Rohlf FJ. 1992. NTSYS-pc: numerical taxonomy and multivariate analysis system. Exeter Software. New York. Available: http:/

Rohlf FJ. 1998. NTSYSpc. numerical Taxonomy and multivariate analysis system. Version 20.02. Department of ecology and evaluation. State University of New York. Stony Brook, New York.

Russell JR, Ellis RP, Thomas WTB, Waugh R, Provan J, Booth A, Fuller J, Lawrence P, Young G, Powell W. 2000. A retrospective analysis of spring barley germplasm development from foundation genotypes’ to currently successful cultivars. Molecular Breeding 6, 553–568.

Salem KFM, El-Zanaty AM, Esmail RM. 2008. Assessing wheat (Triticum aestivum L.) Genetic diversity using morphological characters and microsatellite markers. World Journal of Agricultural Sciences 4, 538-544.

Sharam HC, Gill BS. 1983. Current status of wild hybridization in wheat. Euphytica 32, 17–31.

Spanic, V, Buerstmayr H, Derezner G. 2012. Assessment of genetic diversity of wheat genotypes using microsatellite markers. Periodicum Biologorum 114, 37–42.

Tautz D, Renz M. 1989. Hyper variability of simple sequences as general source of ploymorphic DNA markers. Nucleic Acids Research 17, 6463–6471.

Tsegaye S, Tesemma T, Belay G. 1996. Relationships among tetraploid wheat (Triticum turgidum L.) landraces population revealed by isozyme markers and agronomic traits. Theoretical and Applied Genetics 93, 600-605.

Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV.  1990.   DNA   polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 6231–6235.

Yildirim  A,  Sonmezoglu  OA,  Gokhen  S, Kandemir N, Aydin N. 2011.  Determination  of genetic  diversity  among  Turkish  durum  wheat landraces  by  micro  satellites,  African  Journal  of Biotechnology 10, 3915-3920.

Zhang   P,   Dreisigacker   S,   Buerkert   A, Alkhanjari S, Melchinger AE, Warburton ML. 2006.  Genetic  diversity  and  relationship  of  wheat landraces from oman investigated with SSR markers. Genetic Resources and Crop Evolution 53, 1351-1360.