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Investigation of genetic diversity of some durum and bread wheat genotypes using SSR markers

Reza Mir Drikvand, Goodarz Najafian, Elham, Aram Salahvarzi

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J. Bio. Env. Sci.6(3), 24-32, March 2015

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Abstract

Diversity is very important for breeding objective, since a narrow genetic base of germplasm is very vulnerable to biotic and abiotic stress. Genetic diversity of 40 wheat genotypes was assessed using 30 SSR primers that all of them were generated scorable bands. Totally 71 alleles (ranged between 2 to 4 alleles per each locus) was distinguished. Polymorphic information content (PIC) for all SSR primers was calculated. The highest (0.77) and the lowest (0.13) value of PIC was pertained to Xbarc352 and Xcfd56 Primers, respectively. According to similarity matrix, genetic similarity value ranged from 0.18 to 0.95 with an average of 0.48. The lowest and highest genetic similarity was observed between the Sistan and Arg (Bread wheat, No 27 and 28), Karkheh and Behrang (Durum wheat, No 35 and 38) genotypes respectively. Unweighted pair group method of the arithmetic average (UPGMA), based on Jaccard similarity clustering form a dendrogram with three genotypes group. Clustering somewhat was distinguished durum and bread wheat’s. Principle co-ordinate Analysis (PCA), 2D plot was confirmed the results of cluster analysis. Cophenetic correlation showed that molecular data and cluster was corresponded. It was concluded that SSR marker was suitable for evaluated of genetic diversity in wheat genotypes and this genetic diversity can be used in wheat breeding programs.

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