Investigation of haemato- and hepatotoxic effects of titanium dioxide nanoparticles in male sprague dawley rats

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Research Paper 01/08/2019
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Investigation of haemato- and hepatotoxic effects of titanium dioxide nanoparticles in male sprague dawley rats

Adeel Khalid, Farhat Jabeen, Azhar Rasul, Salma Sultana, Kashif Zahoor, Muhammad Asif Latif
Int. J. Biosci.15( 2), 182-193, August 2019.
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This study investigated the toxic effect of TiO2 NPs on blood and liver tissues of male Sprague Dawley rats. For this purpose, 25 adult male Sprague Dawley rats of 200±5g weight were procured from the animal house of Government College University Faisalabad, Pakistan. Rats were acclimatized for 7 days before the start of study in ventilated cages at (25±2°C) with the approval of ethical committee of Government College University Faisalabad. These rats were randomly divided in to 5 groups. First control (C) with no treatment, 2nd placebo injected with normal saline (S) and three treated groups (G1, G2 and G3). The treated groups were intraperitoneally injected with TiO2 NPs @ 80 or 120 or 160 mg/kg BW of rats for 28 days on alternate day. Animal’s mortality, hematology and liver histology were evaluated. During the experiment no mortality was found while TiO2 NPs exposure showed highly significant pathological changes in haematological parameters and liver function test (p<0.05). The histological alterations were found severe in medium and high dose treated groups as compared to low dose while Control (C) and (S) saline treated groups showed normal histology. Treated groups showed different alterations such as vacuolation, damaging epithelial lining, karyolysis, sinusoids and neutrophilic and lymphocytic infiltration were highly significant in both G2 and G3 treated groups. This study explored the TiO2 -NPs caused toxicity in blood and hepatic tissues. It is concluded from present study that selected dose of titanium dioxide has negative impact on the health of living organisms.


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