Investigation the morphocytological traits and ploidy level in Iris species of Iranian native in Zanjan Province

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Research Paper 01/07/2014
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Investigation the morphocytological traits and ploidy level in Iris species of Iranian native in Zanjan Province

Somayyeh Joz Ghasemi, Vali Rabiei, Ali SoleymanI, Ahmad Khalighi
J. Bio. Env. Sci.5( 1), 72-81, July 2014.
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Determining the chromosomal level is important in breeding programs. Determining the chromosomal levels in Iris species is time consuming and costly by Cytogenetic and DNA Cytometric methods due to the large number and tiny size of chromosomes. Therefore, an investigation was performed in 2011-2013 aimed at assessing the relationship between the morphocytological traits and chromosomal level in lris species native to Iran (Zanjan province) and was arranged in a completely randomized block design with10 replications. Results of the statistical analysis showed that the traits of chloroplast number in stomatal guard cells, length stomatal guard cells, stomatal and epidermal cell density, stomatal index and stomatal conductance cannot be an accurate measure for determining the chromosomal level among different species of Iris under this study. During the implementation of test, Clypeate pollen grains were observed in the species I.persica, I.sp and I.psedocaucasica and the results of the statistical analysis of the data indicated that a significant increase in the pollen grain diameter was observed in species with higher level of chromosome, compared with lris species with lower levels of chromosome, and this trait can be used as a simple and rapid method in determining the chromosomal level of lris species and it is capable of simplifying the implementation of the iris breeding programs.


Abak K. Comlekcioglu N, Buyukalaca S, Sari N. 1998. Use of stomatal characteristics to estimate ploidy level of haploid and diploid pepper plants. Tenth EUCARPIA meeting Capsicum and Eggplant. 12, 179-182.

Alishah O, Omidi M. 2008 . laboratory Methods of Cytogenetics. University of Tehran Press.

Austin C. 2005. Irises a Gardeners Encyclopedia. Portland. U.S.A.

Azimi MH, Moradi Ashori B, Hoseininia A. 2011 . Genetic variation and heritability of Iranian Iris species characters. The Quarterly Journal – Plant Breeding and Genetic Research of pasture and forest of Iran. 19, 153-166.

Azizian D, Sheidai M, Jahanmiri Z. 1993. An anatomical and cytological study of three Iranian Iris species. Pajohesh va Sazandegi. 29, 44-47.

Cenci CA, Pegiatti M, Falistocco E. 1984. Phleum pretense (Gramineae) chromosomal and biometrical analysis of Italian populations. Willdenowia. 14, 343-353.

Cramer CH. 1999. Laboratory techniques for determining ploidy in plants. Hort technology. 594-596.

Donmez EO, Pinar NM. 2001. The clypeate pollen grains of Turkish Iris L. (Iridaceae): subgenus Scorpiris sach. Turk J Bot. 25, 57-62.

Funamota T, Kondo K, Tatarenko Gontcharov IV. 2006. Intraspecific polyploidy of Parnassia palustris Var.multiseta (Saxifragaceae s. l.) collected in Primorye and Altai Territories. Russia. Chromosome botany. 1, 23-26.

Ghahreman A. 1805. Iran floor. Anestitue Science jangal v marta. 7.

Ghanavati F, Eskandari H. 2011. Relationship between the chloroplast number in stomatal guard cells, flow cytometry and ploidy level in Onobrychis spp. Journal Behnejadi Nahal and Bazr 3, 1-27.

Hamill SD, Smith MK, Dodd WA. 1992. In vitro Induction of banana autotetraploids by colchicine treatment of micropropagated diploids. Australian Journal Botanical. 40, 887-896.

Joachimiak   A, Kula A. 1993. Cytotaxonomy and karyotype evolution in Phleum sect. Phleum (Poaceae) in Poland. Plant Systematics and Evolution. 188, 17-30.

Joachimiak A, Kula A. 1996. Karyo system ati cs of the Phleumalpinum polyploid complex (Poaceae). Plant Systematics and Evolution. 203, 11-25.

Joachimiak A, Grabowska-Joachimiak A. 2000. Stomatal cell length and ploidy level in four taxa belonging to the Phleum sect. Phleum. Acta Bolologica Cracoviensia . Ser Botanica. 4, 103-107.

Keri DJ, Reed SM, Timothy A, Rinehart A. 2007. Analysis of Ploidy Level and Its Effects on Guard Cell Length, Pollen Diameter, and Fertility in Hydrangea macrophylla. Horticultural Science. 42, 483-488.

Kim HH, Park YW, Yoon PS, Choi HW, Bang JW. 2004. Karyotype analysis of eight Korean native species in the genus Iris. Korean .Journal Medicinal Crop Science 12, 401-405.

Leech RM. 1981. Observation of the mechanism of chloroplast division in higher plants. New Phytologist. 87, 1-9.

Mishra MK. 1997. Stomatal characteristics at different ploidy levels in Coffea L. Annals of Botany. 80, 689-692.

Murray H, Standing L. 1992. Genomic constancy during the evelopment of Lathyrus odoratus cultivars. Heredity 68, 321-327.

Nordenskiold H. 1945. Cytogenetic studies in the genus Phleum. Acta Agriculturae Suecana. 1, 1-137.

Omidbaigia R, Mirzaeea M, Hassanib ME, Sedghi Moghadam M. 2010. Induction and identification of polyploidy in basil (Ocimum basilicum L.) medicinal plant by colchicine treatment. Plant Production 4, 1735-6814.

Punt W, Blackmore S, Nilson S, Le TA. 1994. Glossary of pollen and spore terminology. Utrecht. LPP Foundation.

Rahimi V. 2008 . Investigate Variety of morphological and chromosomal syndromes Iranian native Iris. Master’s thesis. Department of Plant Production.Tehran university.

Rahimi V, Sadat-Hosseini M, Solymani A, Bahermand N, Meftahizade H. 2011. Assessment of cytological and morphological variation among Iranian native Iris specie. African Journal of Biotechnology 10, 8805-8815.

Reeves A, Tear J. 2000. MicroMeasurc for Windows, version 3.3. Free program distributed by the authors over the Internet from

Rinehart A. 2007. Analysis of Ploidy Level and Its Effects on Guard Cell Length, Pollen Diameter, and Fertility in Hydrangea macrophylla. Horticultural Science 42, 483-488.

Salisbury KE. 1927. On the causes and ecological significance of stomatal frequency with special refrence to the woodland flora. Philosophical transactions of the Royal Society. 216, 575 – 578.

Speckman GJ, POST J, Dijkstra H. 1965. The length of stomata as an indicator for polyploidy in rye-grasses. Euphytica. 14, 225-230.

Teare ID, Peterson CJ, law AG. 1971. Size and frequency of leaf stomata in cultivars of Triticum aestivum and other Triticum species. Crop Science. 11, 496-498.

wendelbo P. 1704. Tulips and Iris by Iran and neighboring species. Botanical Institute. Iran. 83.