Investigation the morphocytological traits and ploidy level in Iris species of Iranian native in Zanjan Province

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Research Paper 01/07/2014
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Investigation the morphocytological traits and ploidy level in Iris species of Iranian native in Zanjan Province

Somayyeh Joz Ghasemi, Vali Rabiei, Ali SoleymanI, Ahmad Khalighi
J. Bio. Env. Sci.5( 1), 72-81, July 2014.
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Abstract

Determining the chromosomal level is important in breeding programs. Determining the chromosomal levels in Iris species is time consuming and costly by Cytogenetic and DNA Cytometric methods due to the large number and tiny size of chromosomes. Therefore, an investigation was performed in 2011-2013 aimed at assessing the relationship between the morphocytological traits and chromosomal level in lris species native to Iran (Zanjan province) and was arranged in a completely randomized block design with10 replications. Results of the statistical analysis showed that the traits of chloroplast number in stomatal guard cells, length stomatal guard cells, stomatal and epidermal cell density, stomatal index and stomatal conductance cannot be an accurate measure for determining the chromosomal level among different species of Iris under this study. During the implementation of test, Clypeate pollen grains were observed in the species I.persica, I.sp and I.psedocaucasica and the results of the statistical analysis of the data indicated that a significant increase in the pollen grain diameter was observed in species with higher level of chromosome, compared with lris species with lower levels of chromosome, and this trait can be used as a simple and rapid method in determining the chromosomal level of lris species and it is capable of simplifying the implementation of the iris breeding programs.

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