Irradiation effect of gamma rays on growth and SDS-PAGE analysis in Panicum sumatrense Roth Ex.

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Research Paper 09/09/2023
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Irradiation effect of gamma rays on growth and SDS-PAGE analysis in Panicum sumatrense Roth Ex.

V. Prabakaran, P. Manivel, S. Parvathi, S. Palanivel
J. Bio. Env. Sci.23( 3), 60-66, September 2023.
Certificate: JBES 2023 [Generate Certificate]

Abstract

An attempt was made to find out the effect of gamma radiation on Panicum sumatrense. The seeds of little millet cv. ATL-1 and CO-2 were exposed to different doses of gamma rays, like 100, 200, 300, 400, 500, and 600 Gy. The seeds treated with gamma rays were sown in plastic pots with red soil to analyze the LD50 value and growth parameters. The lethal dose 50% value was observed at 400 Gy and 500 Gy for CV ATL-1 and CO-2, respectively. In both cultivars, all the growth parameters, such as percentage of germination, percentage of survival, shoot length, root length, fresh weight, and dry weight, showed a decreasing trend towards control with increasing doses of gamma rays, and the reduction was maximum at 600 Gy and minimum at 100 Gy. The cv.ATL-1 seedlings treated with gamma radiation were subjected to estimation of total protein content, and this protein content showed a decreasing trend compared to the control. The number of protein bands and kDa values of cv. ATL-1 were observed by using SDS-PAGE analysis. The kDa value of 132.8 was present only in 400, 500, and 600 Gy. The growth attributes of cultivar ATL-1 were unique compared to cultivar CO-2.

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Arunachalam V, Rengalaksmi R, Kubera Raj MS. 2005. Ecological Stability of genetic diversity among landraces of little millet (Panicum sumatrence) in South India. Genetic Resources and Crop Evolution 52(1), 15-19.

Ashoka P, Bandla, Gangaiah, Sunitha NH. 2020. Millets-Foods of Twenty First Century. International Journal of Current Microbiology and Applied Sciences ISSN 9(12), 2319-7706.

Cholakova N, Stoilova T, Hadjiiska E. 2003.Changes in the seed protein patterns of isogenic pepper lines (Capsicum annuum L.) obtained by gamma rays irradiation of the cultivar ‘Zlaten medal’. Capsicum and Eggplant Newslett (22), 91-94.

Deepika, Minakshi Pal, Pahuja SK. 2016. Morphological variations induced by Ethyl Methane Sulphonate in Cluster bean (Cyamopsis tetragonoloba (L.) Taub.). Forage Res (41), 218-221.

Finney DJ. 1971. Probit analysis, 3rd ed. Cambridge University Press, London p. 318.

Hegazi, Hamideldin. 2010. The effect of gamma irradiation on enhancement of growth and seed yield of okra [Abelmoschus esculentus (L.) Monech] and associated molecular changes. Journal of Horticulture and Forestry Vol. 2(3), pp. 038-051.

Jain SM. 2010. Mutagenesis in crop improvement under the climate change. Rom. Biotechnol, Lett 15(2), 88-106.

Joshi N, Ravindran A, Mahajan V. 2011. Investigation on Chemical Mutagen Sensitivity in Onion (Allium cepa L.). International Journal of Botany (7), 243-248.

Kiong ALP, Lai AG, Hussein S, Harun AR. 2008. Physiological Responses of (Orthosiphon stamineus) Plantlets to Gamma Irradiation. Am-Eurasian J. Sustain.       Agric 2(2), 135-149.

Kovacs E, Keresztes A. 2002. Effect of Gamma and UV-B/C Radiation on Plant Cell. Micron 33, 199-210.

Kulkarni GB. 2011. Effect of mutagen on pollen fertility and other parameters in Horsegram (Macrotylo mauniflorum (Lam.) Verdc). Bio. Sci. discovery (2), 146-150.

Laemmli U. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature (227), 670-685.

Lowry, Rosebrough, Farr, Randal. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem 193, 265-275.

Lu H, Zhang J, Liu KB, Wu N, Li Y, Zhou K. 2009. Earliest domestication of common millet (Panicum miliaceum) in East Asia extended to 10,000 years ago. Proceedings of the National Academy of the United States of America 106, 7367-7372.

Manivel P, Prabakaran V, Parvathi S, Palanivel S. 2022. Effect of gamma rays on M1 attributes and SDS-PAGE analysis in okra [Abelmoschus esculentus (L). Moench]. Journal of Biodiversity and Environmental Sciences (JBES) ISSN: 2220-6663 (Print) 2222-3045 (Online) 21(5), 268-274.

Mohamed IFF. 2000. Characterization of some local varieties of Cucurbitaceae growing under Egytian conditions. M. Sc. Thesis, Faculty of Agriculture, Ain-Shams University.

Muniappan V, Manivel P, Prabakaran V, Palanivel S. 2022. Effect of diethyl sulphate on m1 attributes and sds-page analysis in groundnut Arachis hypogaea L. Plant Archives 22(1), 15-18.

Ramkumar R, Dhanavel D. 2021. Effect of physical and chemical mutagenesis in little millet (Panicum sumatrense Roth Ex) on seed germination, seedling survival through induced mutation. Plant Archives 21(1), 1104-1108.

Rashed MA, Fahmy EM, Sallam MA. 1994. Embryo culture, protein and isozyme electrophoresis as selectable markers to predict salt tolerance in wheat. 5th Conf. Agricultural Development Research Faculty of Agriculture, Ain Shams Univ. Cairo, Egypt (1), 469-490.

Samiullah Khan, Sonu Goyal. 2009. Improvement of mungbean varieties through induced mutations. African Journal of Plant Science 3(8), 174-180.

Talebi AB, Talebi AB, Shahrokhifar B. 2012. Ethyl Methane Sulphonate (EMS) Induced Mutagenesis in Malaysian Rice (cv. MR219) for Lethal Dose Determination. American Journal of Plant Sciences (3), 1661-1665.

Vanniarajan C, Karthikeyan M, Vetriventhan M, Nirmalakumari A, Shanmuga Sundaram R, Sivakumar T, Srinivasan G. 2021. Probit Analysis for Lethality, Injury and Sterility in M1 Generation of MDU 1 Barnyard Millet (Echinochloa frumentacea) Biological Forum- An International Journal 13(4), 1046-1051.