Isolation and characterization of a sporless mutant in the white button mushroom (Agaricus bisporus)

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Research Paper 01/03/2014
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Isolation and characterization of a sporless mutant in the white button mushroom (Agaricus bisporus)

Rasouli Farzad, Motallebiazar Alireza, Bolandnazar Sahebali, Zare Nahandi Fariborz, Panahande Jaber
J. Bio. Env. Sci.4( 3), 156-162, March 2014.
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Despite the long cultivation and importance of Agaricus bisporus, but efforts in breeding of this species are minimal. One of the major reasons for the little efforts is the life cycle. Because of the life cycle the cross cannot happen between single spores of different strains. So variation in Agaricus bisporus is very low and most of strains that are available in the markets are very similar. One of the ways to make variation is using mutagens like UV irradiation. We selected A15 strain that is one of the important strains in Iran. Fragments of mycelium were placed to irradiation by UV lamp 10-w for eight exposure periods (0, 4, 8, 16, 24, 48, 72 and 96 hours) at a distance of 10 cm. In order to pick out of isolate mycelia we examined irradiated fragments after spawning and fruit body production. We archived to pick out one mutated isolate in the exposure of 24 hour that it is different in gill morphology, the rate of spawn running and it cannot produce spores. It is the first report that one sporless isolate of Agaricus bisporus introduced. This study shows that mutagenesis by UV can be useful and a quick way method to make diversity and breeding of Agaricus bisporus.


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