Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | March 1, 2014

VIEWS 1
| Download 3

Isolation and characterization of a sporless mutant in the white button mushroom (Agaricus bisporus)

Rasouli Farzad, Motallebiazar Alireza, Bolandnazar Sahebali, Zare Nahandi Fariborz, Panahande Jaber

Key Words:


J. Bio. Env. Sci.4(3), 156-162, March 2014

Certification:

JBES 2014 [Generate Certificate]

Abstract

Despite the long cultivation and importance of Agaricus bisporus, but efforts in breeding of this species are minimal. One of the major reasons for the little efforts is the life cycle. Because of the life cycle the cross cannot happen between single spores of different strains. So variation in Agaricus bisporus is very low and most of strains that are available in the markets are very similar. One of the ways to make variation is using mutagens like UV irradiation. We selected A15 strain that is one of the important strains in Iran. Fragments of mycelium were placed to irradiation by UV lamp 10-w for eight exposure periods (0, 4, 8, 16, 24, 48, 72 and 96 hours) at a distance of 10 cm. In order to pick out of isolate mycelia we examined irradiated fragments after spawning and fruit body production. We archived to pick out one mutated isolate in the exposure of 24 hour that it is different in gill morphology, the rate of spawn running and it cannot produce spores. It is the first report that one sporless isolate of Agaricus bisporus introduced. This study shows that mutagenesis by UV can be useful and a quick way method to make diversity and breeding of Agaricus bisporus.

VIEWS 1

Copyright © 2014
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Isolation and characterization of a sporless mutant in the white button mushroom (Agaricus bisporus)

Beejan PHF, Nowbuth R. 2009. Use of irradiation for the induction of mutations in oyster mushrooms for improvement of strains. Induced plant mutations in the genomics Era. Food and agriculture organization of the United Nation, Rome, 289-292.

Brombery SK, Schwalp MN. 1977. Isolation and characterization of temperature sensitive sporulationless mutants of the basidomycete Schizophyllun commune. Canadian Journal Genetic Cytology 19, 447.

Chang S, Miles PG. 2004. Mushrooms Cultivation, Nutritional Value, Medicinal Effect and Environmental Impact. Second Edition. CRC Press, 105-127.

Djajanegara I, Harosyo. 2008. White oyster mushroom (Pleurotus florida) mutant with altered antioxidant contents. Biotropia 15(1), 65-73.

Djajanegara I, Harosyo. 2008. Mutation study on white oyster mushroom (pleurotus floridae) using gamma irradiation. Journal of chemical and natural resources engineering 4(1), 12-21.

Eger G, Eden G, Wissing E. 1976. Pleurutus ostreatus breeding potential of a new cultivated mushroom. Theoretical Applied Genetic 47, 155-163.

Fahad AQ, Salim K. 2009. Mutagenesis effects of sodium azide and its application in crop improvement. World Applied science journal 6, 1589-1601.

Elliott TJ, Langton FA. 1981. Strain improvement in the cultivated mushroom Agaricus bisporus. Euphytica 30, 175-182.

Goltapeh ME, Danesh YR, kamal S, Varma A. 2009. Chapter 26: Biology and molecular approaches in genetic improvement of cultivated button mushroom (Agaricus bisporus), 403-421.

Hasebe K, Murakami S, Tsuneda A. 2007. Cytology and genetics of a sporless mutant of Lentinula edodes. Mycologi 83, 354-359.

Horgen PA, Anderson JB. 1993. Edible mushroom. In advance in mushroom biotechnology, M. C. Nair, C. G. Luludas (ed.), Jodhpur, Scientific Pub, 447-462.

Liu Z, Zhang K, Lin J, Guo L. 2011. Breeding cold tolerance strain by chemical mutagesis in Volvariella Volvacea. Scientia Horticulturae 130, 18-24.

Murakami S. 1993. Genetics and breeding of spore-deficient strains in Agrocybe cylindracea and Lentinus edodes. Mushroom biology and mushroom products, 63-69.

Obatake Y, Murakami S, Matsumoto T, Fukumasai-Nakai Y. 2003. Isolation and characterization of a spore less mutant in Pleurotus eryngii. Mycoscience 44, 33-40.

Pandey M, Tewari RP. 1993. Strategies for selection and breeding of edible mushrooms. In advance in mushroom biotechnology, M. C. Nair, C. G. Luludas, (ed.), Jodhpur, Scientific Pub, 61-69.

Raper CA, Raper JR, Miller RE. 1972. Genetic analysis of the life cycle of Agaricu bisporus. Mycologia 64, 1088-117.

Ravishankar S, Pandy M, Tewari RP, Krishna V. 2006. Development of spore less/ low sporing strains of Pleurotus through mutation. World Journal of Microbiology & Biotechnology 22,1021-1025.

Royce DJ, May B. 1982a. Genetic relatedness and its application in selective breeding of Agaricus brunnesceens. Mycologia 74, 569-575.

Shimomura N, Hasebe K, Murakami S, Matsumoto T, Fukumasai-Nakai, Y. 2007. Isolation of a homothallic mutant in Lentinula edodes. Mycosciense 48, 117-121.

Sonnenberg ASM, Baars JJP, Hendrickx P M, Kerrigan RW. 2005. Breeding mushrooms: State of the Art. The 5th International Conference on Mushroom Biology and Mushroom Products (ICMBMP5), 167-173.

Sonnenberg ASM, Baars JP, Hendrickx PM, Lavrijssen B, Gao W, Weijn A. Mes JJ. 2011. Breeding and strain protection in the button mushroom Agaricus bisporus. In: Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products (ICMBMP7), 7-15.

SUBMIT MANUSCRIPT

Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background