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Isolation and Characterization of Acidophilic Sulphur and Iron Oxidizing Acidithiobacillus ferrooxidans from Black Shale

Shahroz Khan, Faizul Haq

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Int. J. Biosci.2(2), 85-94, February 2012


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In present study, the acidiphilic sulphur and Iron oxidizing Bacterium Acidithiobacillus ferrooxidans were isolated and characterized from black shale grown on solid and liquid medium. The specimens were taken from the black shale, Tarbela (Gazi) and water samples from Chamyari Pakistan. The indigenous strain of Acidithiobacillus ferrooxidans were Gram-negative, motile, and rod-shaped bacteria. Acidithiobacillus ferrooxidans oxidized iron andreduced sulfur compounds like thiosulfate and tetrathionate. The bacterial oxidation of pyrite and reduced sulfur compounds produced sulfuric acid. Different biochemical activities like starch hydolysis, gelatin hydrolysis, hydrogen sulfide production, catalase reaction, urease test, indole production, methyl red test, voges proskaur, citrate utilization and triple sugar iron test of the isolates were performed and picturized. Some carbon sources like glucose, sucrose, fructose, raffinose, d-sorbitol, galactose, lactose, maltose, rahammanose and mannose were given to check the growth of isolates.


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Isolation and Characterization of Acidophilic Sulphur and Iron Oxidizing Acidithiobacillus ferrooxidans from Black Shale

Dopson M, Baker-Austin C, Ram Kopponeedi, P, Bond P. 2003. Growth in sulfidic mineral environments, metal resistance mechanisms in acidophilic micro-organisms. Microbiol; 149, 1959-1970.

Farbiszewska, T., Kiczma, J. and Bak, M. (2004). Biological extraction of metals from polish black shale. Physicochemical Problems of Mineral Processing 37: 51-57.

Grobelski T, Kiczma FJ, Farbiszewka T. 2007. Bioleaching of polish black shale. Physicochemical Problems of Mineral Processing 41, 259-264.

Karavaiko G, Rossi D, Agate A, Groudev S, Avakian Z. 1989. Biogeotechnologija metalov Moskva, s.338.

Kelly DP, Wood AP. 2000. Reclassification of some species of Thiobacillus to the newly designated genera Acidithiobacillus gen. Nov., Halothiobacillus gen. nov. and Thermithiobacillus gen. nov. Internacional Journal of Systematic and Evolutionary Microbiology 50, 511-516.

Khalid AM, Bhatti TM, Umar M. 1993. An improved solid medium for isolation, enumeration and genetic investigations of autotrophic iron – and sulfur- oxidizing bacteria . Appl Microbiol Biotechnol. 39(2), 259-263.

Kiczma  FJ, Farbiszewska T, Bak  M. 2004. Bioleaching of metals from black in neutral medium. Physicochemical Problems of Mineral Processing 382, 73-280.

Olson   GJ, Brierley JA, Brierley CL. 2003. Progress in bioleaching: applications of the microbial processes by the mineral industries. Appl Microbiol Biotechnol. 63, 249-257.

Pronk JT, Meijer WM, Haseu W, Dijken JP, Bos P, Kuenen JG. 1991. Growth of Thiobacillus ferrooxidans on formic acid. Appl Environ Microbiol. 57, 2057-2062.

Puhakka J, Tuovinen OH. 1986. Microbiological leaching  of  sulfide  minerals  with  different  regimes. Appl Microbiol Biotechnol. 24, 144-148.

Ramirez P, Guiliani N, Valenzuela L, Beard S, Jerez CA. 200). Differential protein expression during growth of Acidithiobacillus ferrooxidans on ferrous iron, sulfur compounds or meta sulphides. Appl Environ Microbiol. 70, 4491-4498.

Rawlings DE, Dew D, Plessis C. 2003. Biomineralization of metal containing ores and concentrates. Trends in Biotechnology 21, 38–44.

Rohwerder T, Gehrke T, Kinzler K, Sand W. 2003. Progress in bioleaching: fundamentals and mechanisms of bacterial metal sulfide oxidation. Appl Microbiol Biotechnol. 63, 239-248.

Sugio T, Wakabayashi M, Kanao T, Takeuchi  F. 2008. Isolation and Characterization of Acidithiobacillus ferrooxidans Strain D3-2 Active in Copper Bioleaching from a Copper Mine in Chile Biosci. Biotechnology. Biochem 72(4), 998–100.

Torma AE. 1977. The Role of Thiobacillus ferrooxidans in hydrometallurgical. Processes. Advances in Biochemical Engin. Springer-Verlag, Berlin, 1–37.

Tuovinen OH, Niemela SI, Gyllenberg HG. 1971. Effect of mineral nutrients and organic substances on the development of Thiobacillus ferrooxidans. Biotechnol Bioeng. 13, 517-527.


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