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Research Paper | February 1, 2012

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Isolation and Characterization of Acidophilic Sulphur and Iron Oxidizing Acidithiobacillus ferrooxidans from Black Shale

Shahroz Khan, Faizul Haq

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Int. J. Biosci.2(2), 85-94, February 2012

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Abstract

In present study, the acidiphilic sulphur and Iron oxidizing Bacterium Acidithiobacillus ferrooxidans were isolated and characterized from black shale grown on solid and liquid medium. The specimens were taken from the black shale, Tarbela (Gazi) and water samples from Chamyari Pakistan. The indigenous strain of Acidithiobacillus ferrooxidans were Gram-negative, motile, and rod-shaped bacteria. Acidithiobacillus ferrooxidans oxidized iron andreduced sulfur compounds like thiosulfate and tetrathionate. The bacterial oxidation of pyrite and reduced sulfur compounds produced sulfuric acid. Different biochemical activities like starch hydolysis, gelatin hydrolysis, hydrogen sulfide production, catalase reaction, urease test, indole production, methyl red test, voges proskaur, citrate utilization and triple sugar iron test of the isolates were performed and picturized. Some carbon sources like glucose, sucrose, fructose, raffinose, d-sorbitol, galactose, lactose, maltose, rahammanose and mannose were given to check the growth of isolates.

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Isolation and Characterization of Acidophilic Sulphur and Iron Oxidizing Acidithiobacillus ferrooxidans from Black Shale

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