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Isolation and characterization of arsenic resistant bacteria, isolated from industrial wastewater of Pakistan region and bacterial biomass bioremediation activity in arsenic bioremediation

Research Paper | September 1, 2019

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Shahid Sher, Abdul Rehman

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Int. J. Biosci.15( 3), 334-343, September 2019

DOI: http://dx.doi.org/10.12692/ijb/15.3.334-343


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Arsenic is a toxic metalloid and ubiquitous, means found everywhere on earth. Due to toxicity it is necessary for scientist to remove or reduce it from the environments. So through bioremediation, by using the bacteria arsenic can be reduced from the environments. In this present study arsenic resistant bacterium was isolated from wastewater of industrial origin, in District Sheikhupura, Pakistan. Optimum growth conditions, growth curve, minimum inhibitory concentration regarding arsenic and other heavy metals, biochemical testing, 16S rRNA analysis,  glutathione and non-protein thiol contents and bacterial biomass bioremediation activity was determined for isolated bacterial strain. The isolated strain showed best growth at 37 °C and pH 7. The minimum inhibitory concentration (MIC) regarding arsenite and arsenate in isolated strain was 32 mM and 220 mM respectively. Cross metals resistance profile was ( Pb; 6 mM, Cd; 5 mM, Cr; 6 mM, Hg; 2 mM, Se; 6 mM, Co; 2 mM and Ni; 2.5 mM ). On 16S rRNA sequence and biochemical basis bacteria was closely related to Staphylococcus warner Strain AW 25. The significant alternation in reduced glutathione level was observed under 15 mM arsenite stress. The ratio of GSH and GSSG was increased 33.33 % while Non-protein thiol was increased 55.55 % due to 15 mM arsenite stress in isolated bacterial strain. The bioremediation efficiency of isolated bacterial biomass was 92 % after 10 h. So, due to its better bioremediation activity, the isolated bacterial strain can be used in the bioremediation of arsenic from arsenic contaminated sites.


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Isolation and characterization of arsenic resistant bacteria, isolated from industrial wastewater of Pakistan region and bacterial biomass bioremediation activity in arsenic bioremediation

Dey U, Chatterjee S, Mondal NK. 2016. Isolation and characterization of arsenic-resistant bacteria and possible application in bioremediation. Biological reports 10, 1-7.

James C, Natalia S. 2014. Microbiology. A labortory manual: Pearson Education.

Koechler S, Cleiss-Arnold J, Proux C, Sismeiro O, Dillies MA, Goulhen-Chollet, F, Hommais F, Lièvremont D, Arsène-Ploetze F, Coppée JY. 2010. Multiple controls affect arsenite oxidase gene expression in Herminiimonas arsenicoxydans. BMC microbiology 10, 53-55.

Li X, Zhang L, Wang G. 2014. Genomic evidence reveals the extreme diversity and wide distribution of the arsenic-related genes in Burkholderiales. PloS one 3, e99236.

Manzoor M, Abid R, Rathinasabapathi B, De-Oliveira LM, Da-Silva E, Deng F, Rensing C, Arshad M, Gul I, Xiang P. 2019. Metal tolerance of arsenic-resistant bacteria and their ability to promote plant growth of Pteris vittata in Pb-contaminated soil. Science of the Total Environment 660, 18-24.

Mohanty D. 2017. Conventional as well as emerging arsenic removal technologies—a critical review. Water, Air, & Soil Pollution 10, 381.

Mondal M, Biswas JK, Tsang YF, Sarkar B, Sarkar D, Rai M, Sarkar SK, Hooda PS. 2019. A wastewater bacterium Bacillus sp. KUJM2 acts as an agent for remediation of potentially toxic elements and promoter of plant (Lens culinaris) growth. Chemosphere 232, 439-452.

Mujawar SY, Shamim K, Vaigankar DC, Dubey SK. 2019. Arsenite biotransformation and bioaccumulation by Klebsiella pneumoniae strain SSSW7 possessing arsenite oxidase (aioA) gene. BioMetals 1, 65-76.

Muller D, Lievremont D,  Simeonova DD, Hubert JC, Lett MC. 2003. Arsenite oxidase aox genes from a metal-resistant β-proteobacterium. Journal of bacteriology 185, 135-141.

Naureen A, Rehman A. 2016. Arsenite oxidizing multiple metal resistant bacteria isolated from industrial effluent: their potential use in wastewater treatment. World Journal of Microbiology and Biotechnology 8, 133.

Ng KS, Ujang Z, Le-Clech P. 2004. Arsenic removal technologies for drinking water treatment. Reviews in Environmental Science and Biotechnology 3, 43-53.

Oremland RS, Stolz JF. 2003. The ecology of arsenic. Science 300, 939-944.

Prasad KS, Ramanathan AL, Paul J, Subramanian V, Prasad R. 2013. Biosorption of arsenite (As+ 3) and arsenate (As+ 5) from aqueous solution by Arthrobacter sp. Biomass. Environmental technology 19, 2701-2708.

Qin J, Rosen B, Zhang Y, Wang G, Franke S, Rensing C. 2006.  Arsenic detoxification and evolution of trimethylarsine gas by a microbial arsenite S-adenosylmethionine methyltransferase. Proceedings of the National Academy of Sciences 7, 2075-2080.

Rosen BP. 2002. Biochemistry of arsenic detoxification. FEBS letters 529, 86-92.

Sher S, Rehman A. 2019. Use of heavy metals resistant bacteria—a strategy for arsenic bioremediation. Applied microbiology and biotechnology 103, 6007-6021.

Sher S, Rehman A, Hansen LH, NielsenTK. 2019. Complete Genome Sequences of Highly Arsenite-Resistant Bacteria Brevibacterium sp. Strain CS2 and Micrococcus luteus AS2. Microbiology resource announcements 8, e00531-19.

Smith AH, Lingas EO, Rahman M. 2000. Contamination of drinking-water by arsenic in Bangladesh: a public health emergency. Bulletin of the World Health Organization 78, 1093-1103.

Tariq A, Ullah U, Asif M, Sadiq I. 2019. Biosorption of arsenic through bacteria isolated from Pakistan. International Microbiology 22, 59-68.

Zhang Y, Chen S, Hao X, Su J, Xue X, Yan Y, Zhu YG, Ye J. 2016. Transcriptomic analysis reveals adaptive responses of an enterobacteriaceae strain LSJC7 to arsenic exposure. Frontiers in Microbiology 7, http://dx.doi.org/10.3389/fmicb.2016.00636