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Research Paper | September 1, 2019

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Isolation and characterization of arsenic resistant bacteria, isolated from industrial wastewater of Pakistan region and bacterial biomass bioremediation activity in arsenic bioremediation

Shahid Sher, Abdul Rehman

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Int. J. Biosci.15(3), 334-343, September 2019

DOI: http://dx.doi.org/10.12692/ijb/15.3.334-343

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Abstract

Arsenic is a toxic metalloid and ubiquitous, means found everywhere on earth. Due to toxicity it is necessary for scientist to remove or reduce it from the environments. So through bioremediation, by using the bacteria arsenic can be reduced from the environments. In this present study arsenic resistant bacterium was isolated from wastewater of industrial origin, in District Sheikhupura, Pakistan. Optimum growth conditions, growth curve, minimum inhibitory concentration regarding arsenic and other heavy metals, biochemical testing, 16S rRNA analysis,  glutathione and non-protein thiol contents and bacterial biomass bioremediation activity was determined for isolated bacterial strain. The isolated strain showed best growth at 37 °C and pH 7. The minimum inhibitory concentration (MIC) regarding arsenite and arsenate in isolated strain was 32 mM and 220 mM respectively. Cross metals resistance profile was ( Pb; 6 mM, Cd; 5 mM, Cr; 6 mM, Hg; 2 mM, Se; 6 mM, Co; 2 mM and Ni; 2.5 mM ). On 16S rRNA sequence and biochemical basis bacteria was closely related to Staphylococcus warner Strain AW 25. The significant alternation in reduced glutathione level was observed under 15 mM arsenite stress. The ratio of GSH and GSSG was increased 33.33 % while Non-protein thiol was increased 55.55 % due to 15 mM arsenite stress in isolated bacterial strain. The bioremediation efficiency of isolated bacterial biomass was 92 % after 10 h. So, due to its better bioremediation activity, the isolated bacterial strain can be used in the bioremediation of arsenic from arsenic contaminated sites.

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Isolation and characterization of arsenic resistant bacteria, isolated from industrial wastewater of Pakistan region and bacterial biomass bioremediation activity in arsenic bioremediation

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