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Isolation and diagnosis of Pseudomonas aeruginosa from burn patients

Noor M. Mahmood, Mohammed J. Alwan

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Int. J. Biosci.15( 1), 423-431, July 2019

DOI: http://dx.doi.org/10.12692/ijb/15.1.423-431


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The aim of this study was find out the zoonotic Pseudomonas aeruginosa of patient suffering from burns wound. The purpose of isolating these bacteria from burn patients is the high mortality rate in my country.  (69) Samples were collected from teaching medical Al- Kendi hospital, during a period from October (2007) to June (2008). The samples cultured on blood agar media and incubated at (37 °) C for (24) h. These   colonies were culture on the Blood agar; some of colony cultures on MacConky agar, after that subculture on Pseudomonas agar base. The patients divided into non mature group, their aged ranged from (3-11) years and mature group, their age ranged from (12-58) years, each group includes male, and females. The results showed (9) isolates of Pseudomonas aeruginosa were isolated from (69) burn wound samples (24) samples from kids (13) males and (11) females. (45) Samples collect from adults (20) sample from male and (25) samples from female. The percentage of bacterial isolation was (13%). Females showed higher percentage (7.2%) than males (5.7%) high percentage of bacterial isolates were rusticated at mature group (11.1%) compared with non-mature group (16.6%). The results of serotyping were P16 (33%). P15 (11.1%), P2 (11.1%), P9 (11.1%), P11 (11.1%) , P12 (11.1%). The serotype (P16) is prevailing among the strain classified. The present study suggested that Pseudomonas aeruginosa play important role in contamination of burn wound in human. Many burn patients die not because the burn but because the virulence of bacteria especially Pseudomonas Aeruginosa.


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Isolation and diagnosis of Pseudomonas aeruginosa from burn patients

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