Isolation and diagnosis of Pseudomonas aeruginosa from burn patients

Paper Details

Research Paper 01/07/2019
Views (304) Download (14)

Isolation and diagnosis of Pseudomonas aeruginosa from burn patients

Noor M. Mahmood, Mohammed J. Alwan
Int. J. Biosci.15( 1), 423-431, July 2019.
Certificate: IJB 2019 [Generate Certificate]


The aim of this study was find out the zoonotic Pseudomonas aeruginosa of patient suffering from burns wound. The purpose of isolating these bacteria from burn patients is the high mortality rate in my country.  (69) Samples were collected from teaching medical Al- Kendi hospital, during a period from October (2007) to June (2008). The samples cultured on blood agar media and incubated at (37 °) C for (24) h. These   colonies were culture on the Blood agar; some of colony cultures on MacConky agar, after that subculture on Pseudomonas agar base. The patients divided into non mature group, their aged ranged from (3-11) years and mature group, their age ranged from (12-58) years, each group includes male, and females. The results showed (9) isolates of Pseudomonas aeruginosa were isolated from (69) burn wound samples (24) samples from kids (13) males and (11) females. (45) Samples collect from adults (20) sample from male and (25) samples from female. The percentage of bacterial isolation was (13%). Females showed higher percentage (7.2%) than males (5.7%) high percentage of bacterial isolates were rusticated at mature group (11.1%) compared with non-mature group (16.6%). The results of serotyping were P16 (33%). P15 (11.1%), P2 (11.1%), P9 (11.1%), P11 (11.1%) , P12 (11.1%). The serotype (P16) is prevailing among the strain classified. The present study suggested that Pseudomonas aeruginosa play important role in contamination of burn wound in human. Many burn patients die not because the burn but because the virulence of bacteria especially Pseudomonas Aeruginosa.


Cherringtou VA, Gildow EM. 1981. Bovine mastitis caused by Pseudomonas aeruginosa. Journal of the American Veterinary Medical Association (79), 803-808.

Birch RR, Benner JW. 1992. Pseudomonas pyocyaneus as a factor in pneumonia in swine. Cornell Veterinary 176(10), 189.

Palleroni N. 1984. Psedomonaceae: In: Krieg NR, ed Bergy’s manual of systemic bacteriology, (I), Baltimore: Williames and Wilkins, 141.

Govan JR. 1996. Pseudomonas and nonfermenters. In: green wood, D; Slack, R.C and Peutherer, J.F (eds). Medical Microbiology ilsed, 248-289. Asia ltd. Hongkong.

Greenwood D, Slack R, Peutherer J. 1998. Medical Micrbiology. 15th Ed. Churchill Living Stone. Inc.

Frazier WC, Wasthoff DC. 1985. Food microbiology. 3rd ed. P. (49), Tata McGraw-Hill Publishing Company (Limited. New Delhi).

Gori A, Espinassa F, Deplano A, Nonhaff C, Nicolas MH, Struelens MJ. 1996. Comparison of pubsed- field electrophorsis and randomly amplified Klebsiella pneumoniae Clin. Microbiol 34(10), 2448-2453.

Jawetz E, Melnick JL, Adeberg EA. 1987. In Medical Microbiology. Appleton and 17th ed auge, Norwalk connec ticut. Los. Atlos. California, p 247-250.

Boneiw J. 1970. L. from and Pseudomonas aeruginosa, the etiologic agent of bacterial endocarditis in a dog. Veterinary Medicine (65), 244-255.

Quinn PJB, Carter ME, Markey PK, Carter JR. 2006. Clinical veterinary microbiology. London Mosby-Wolf. P 284-286.

Ohman DE, Sadoff JC, Iglewski BH. 1980. Toxin- A deficient metants of Pseudomonas aeruginosa. Infect. (28), 897-908.

Atlas RM. 1995. Principles of Microbiology, 1st ed. Mos by, Inc. Missouri, P (364).

Bayer AS, Speert DP, Park S, Tu J, Witt MCC. Nast CC, Norman DC. 1991. Functional role of mucoid exopolysaccharide (alginate) in antibiotic-induced and polymorphonuclear leukocyte- mediated killing of Pseudomonas aeruginosa. Infect. Immun., (59), 302-8.

Budzikiewicz H. 2001. Siderophores of the human pathogenic fluorescent Pseudomonas. Curr Top Med. Chem (1), 1-6.

Hancock RE, Worobec EA. 1998. Outer membrane proteins, In: T.C. Montie (ed.), Pseudomonas. Plenum Press New York, P 139-167.

Meyer JM, Stintzi A, Georges C, Holder IA. 1996. Pyoverdin is essential for virulence of Pseudomonas aeruginosa. Infect immune (64), 518-23.

Vasil ML. 1986. Pseudomonas aeruginosa, Biology echanism of virulence, epidemiology. Pediat. (108), 800-805.

Chen ZP, Hu JS DY, Wang WG, Wang JM, He QL. 1987. Preliminary report on the serotyping of IgG isolated from Pseudomonas aeruginosa from Wild and domestic animals Chines Journal of Veterinary Medicine (13), 2-4.

Hoadely AW. 1975. Preliminary studies of fluorescent Pseudomonas capable of growth at 41 C in Swimming pool Waters. Appl. Microbiol (29), 527-531.

Holt JG, kreig NR, Sneash PH, Staley JT, Williams ST. 1994. G-ve aerobic microphillic rods and cocci. In: Bergey’s Manual of Determinative bacteriology 9th (ed.). Williams and Wilkins, USA., P 168-171.

Levinson W, Jawetz E. 1996. G (-ve) rods related to the enteric tract. In.: Examination and broad review medical microbiology and immunology, Prentice Hall International, London, P 94-112.

Timoney JF, Gillespie JH, Scott FW,  Barlough JE. 1988. (8th Ed.). Hagen and Burner`s Microbiology and infectious disease of domestic animals. By Cornell university Press, London, P 35-38.

Hawkey PM, lewis DA. 1989. Medical Bacteriology a practical apporch. IRL. Press. Oyford.

Ayliff GA. 1978. The application of typing methods to nosocomial infection. Methods in Microbiology 10, 39-59.

Swaddi WW, Taugk Tchol T, Silarug M. 1995. An. Out breaks of P.A post-operative endphthalmitis caused by contaminated intra, cular irrigating solution. Trans, Royal Society of Tropical Medicine and Hygiene (89), 28-32.

Rastegar LA, Bahrami HH, Alaghaeh BR. 1998. Pseudomonas infection Tohid Burn center. Iran – Burns (24), 637-641.

Ergin C, Mutlu G. 1999. Clinical distribution and antibiotic resistance of Pseudomonas species. Eastern Journal of Medicine (4), 65-69.

Savas L, Duran N, Savas N, Onlen Y, Ocak S. (2005). The prevalence and resistance patterns of Pseudomonas aeruginosa in intensive care units in a university hospital. Turkish Journal of Medical Science (35), 317-322.

Al-Roubaeay DA. 2002. Bacteriological study on Pseudomonas aeruginosa isolated from postoperative wound infection and their susceptibility to antimicrobial agents. A thesis of M.D. submitted to College of Veterinary medicine, University of Baghdad.

Zaragoza R, Artero A, Camarena JJ, Sancho S, Gonzalez R. 2002. No inadequate empirical antimicrobial treatment on patients with blood stream infection. Clinical Microbiology Infection (9), 412-418.

Clark NM, Patterson J, Lynch JP. 2003. Antimicrobial resistance among gram- negative organisms in the intensive care unit current opinion in critical care (9), 413- 423.

Revathi G, Shannon KP, Stapleton PD, Jain BK, French GL. 1998. Spectrum, betal-lactamase-producing Salmonella senftenberg in burns ward. J. Hospital. [Pub Med].

Holder IA, Schwab M, Jackson L. 1993. Eighteen months of routine topi testing of isolates from burn patients: results and conclusions. J. Antimicrob. Chem.

Drost AC, Burleson DG, Cioffi WG, Mason AD, Pruitt BA. 1993. Plasma cytokines after their relationship to infection. Ann. Surg 218, 74-78.

Mason AD, McManus AT, Pruitt BA. 1986. Association of burn mortality and bacterimia. A25 years review. Arch. Surg. (121), 1027-1031.

Hostacha A, Mita V. 1997. Serotyping and virulence factors of Pseudomonas aeruginosa clinical isolates. Acta. Microbiol. Immunol. Hang. 44.

Myrviik QN, Weiser RS. 1988. Fundamental of medical bacteriology and mycology. 2nd Ed. Lea and Febiger, Philadelphia, P 363-372.