Isolation and Identification of Hibiscus Chlorotic Ringspot Virus (HCRSV) infecting Gumamela (Hibiscus rosasinensis) in the Philippines

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Research Paper 01/02/2016
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Isolation and Identification of Hibiscus Chlorotic Ringspot Virus (HCRSV) infecting Gumamela (Hibiscus rosasinensis) in the Philippines

Lolita M. Dolores, Maricel C. Gonzales, Yron M. Retuta, Pablito M. Magdalita
Int. J. Biosci.8( 2), 149-158, February 2016.
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Abstract

Gumamela (Hibiscus rosa-sinensis L.) is a malvaceous ornamental plant commonly grown in the Philippines. Virus-like symptoms such as chlorotic spots, mottling, vein banding and clearing, vein enation, chlorosis, rosetting, leaf distortion, flower abortion and distortion and stunting were observed on gumamela plants growing in the hibiscus breeding blocks at the Institute of Plant Breeding (IPB).. A new virus, Hibiscus chlorotic ringspot virus (HCRSV), a member of the genus Carmovirus was isolated and identified to be infecting gumamela plants by mechanical inoculation using the sap from symptomatic leaves. The virus isolate caused chlorotic lesions to Chenopodium quinoa, C. amaranticolor, C. murale and Abelmoschus esculentus. However, it did not induce any symptoms in Gomphrena globosa, Nicotiana glutinosa, Physalis floridana, Datura stramonium, Lycopersicon esculentum and Capsicum annuum. Gumamela plants (IPB Accessions No. 95 and 97 and the variety “Superstar”) showing chlorotic ringspots and the symptomatic indicator plants tested positive for HCRSV by Enzyme-linked immunosorbent assay (ELISA). The Reverse Transcriptase Polymerase Chain Reaction (RT-PCR ) using primers that amplify a conserved region in the coat protein (CP) gene of HCRSV giving an amplification with a size of 557bp further confirmed the results. Nucleotide sequence analysis of the CP gene of the HCRSV Philippines showed 97.5 to 97.9% similarity to the HCRSV isolates of Iran, New Zealand and Singapore. It is most related to the Israel isolate with 98.1% identity and less similar with HCRSV-Taiwan with only 93% sequence identity. To our knowledge, this is the first report of HCRSV in gumamela in the Philippines.

VIEWS 90

Brunt A, Crabtree K, Gibbs A. 2000. Viruses of tropical plants. CAB International. Redwood Press Ltd. Melksham, Wiltshire. p. 707.

Brunt A, Spence NJ. 2000. The natural occurrence of Hibiscus chlorotic ringspot virus (Carmovirus; Tombusviridae) in aibika or bele (Abelmoschus manihot) in some South Pacific Island countries. New Disease Reports 11. http://dx.doi.org/10.1046/j.1365-3059.2000.00501.x

De Stradis A, Parella G, Vovlas C, Ragozzino, A. 2008. Vein yellowing of Hibiscus rosa-sinensis caused by Eggplant mottled dwarf virus in Southern Italy. Journal of Plant Pathology 90(2), 359-361.

Dolores LM, Pateña LF. 2009. Isolation and identification of a potyvirus infecting garlic (Allium sativa L.) and shallot (Allium cepa L.). Journal of Tropical Plant Patholology 44, 66-69.

Dolores LM, Gonzales MC, Maghirang RM. 2013. Incidence and distribution of Squash leaf curl Begomovirus in the Philippines. Journal of Tropical Plant Pathology 49(1), 62-70.

Huang M, Koh DCY, Weng LJ, Chang ML, Yap YK, Zhang L, Wong SM. 2000. Complete nucleotide sequence and genome organization of Hibiscus chlorotic ringspot virus, a new member of the genus Carmovirus: evidence for the presence and expression of two novel open reading frames. Journal of Virology 74, 3149–3155. http://dx.doi.org/10.1128/JVI.74.7.3149-3155.2000

Lana AO. 1974. A new ringspot virus disease in Hibiscus rosa-sinensis. Plant Disease Report 58, 1040-1042.

Li SC, Chang YC. 2002. First report of Hibiscus chlorotic ringspot virus in Taiwan. Plant Pathology 51: 803. http://dx.doi.org/10.1046/j.1365-3059.2002.00775.x

Liu FL, Chen TC, Yeh SD, Hsu HT, Chen CC, Bau HJ, Chen YK. 2004. Serological and molecular characterizations of a Hibiscus-infecting Tobamovirus in Taiwan. Plant Pathology Bulletin 13, 283-290.

Luria N, Reingold V, Lachman O, Dombrovsky A. 2013. Full genome sequence of Hibiscus chlorotic ringspot virus from Israel. Genome Announcements 1, 6. http://dx.doi.org/10.1128/genomeA.01050-13

Magdalita PM, Pimentel RB. 2013. Development of Hibiscus hybrids “Women in Public Services Series II” and propagation studies on Hibiscus rosa-sinensis “Cynthia A. Villar”. Philippine Science Letters 6(1), 39-56.

Pourrahim R, Ghobakhlo A, Farzadfar S. 2013. Biological and molecular detection of Hibiscus chlorotic ringspot virus infecting Hibiscus rosa-sinensis in Iran. Phytopathologia Mediterranea 52(3), 528-531. http://dx.doi.org/10.14601/Phytopathol_Mediterr-11554

Rajeshwari R, Reddy RVC, Maruthi MN, Colvin J, Seal SE, Muniyappa V. 2005. Host range, vector relationships and sequence comparison of a Begomovirus infecting hibiscus in India. Annals of Applied Biology 147, 15-25. http://dx.doi.org/10.1111/j.1744-7348.2005.00005.x

Tang    J, Elliott DR, Quinn BD, Clover GRG, Alexander  BJR.  2008.  Occurrence  of  Hibiscus chlorotic  ringspot  virus  in  Hibiscus  spp.  in  New Zealand. Plant Disease 92(9), 1367. http://dx.doi.org/10.1094/PDIS-92-9-1367A

Waterworth HER, Lawson RH, Monroe RL. 1976. Purification and properties of Hibiscus chlorotic  ringspot  virus.  Phytopathology  66, 570-575. http://dx.doi.org/10.1094/Phyto-66-570

Wong SM, Ching CG. 1992. Occurrence of Hibiscus chlorotic ringspot virus in Singapore. Phytopathology 82(6), 722.