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Isolation and Identification of Keratin Degrading (keratinolytic) Bacteria from Poultry Feather Dumping Sites

Sunil Chhimpa, Chandra Shekhar Yadav, P.J. John

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J. Bio. Env. Sci.8(6), 109-119, June 2016

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Abstract

Poultry industries generate a large quantity of feather waste. These feathers contain high quantity of keratin which can be bio-converted into peptides and amino acids using bacteria to make value added products. Therefore, the aim of this study was to isolate and identify keratinolytic bacteria from poultry feather dumping sites at Jaipur city, Rajasthan, India. The samples such as soil and feathers were collected and screened for keratinolytic bacteria. The samples were inoculated on the feather meal agar plates and incubated at 35o C for 24-48 hrs. The culture obtained from the feather meal agar plate was sub cultured on feather meal broth. The bacterium which showed maximum kertainolytic activity was subjected to morphological and biochemical tests. Molecular characterization of the isolated bacteria was done by 16S rRNA sequencing and identified as Bacillus subtilis strain AJ. Culture conditions such as pH, incubation temperature, agitation and duration were optimized for maximum feather degradation. The bacteria B. subtilis showed maximum feather degradation at pH 7 at 300 C with an agitation of 140 rpm for 96 hrs. Isolation, purification and characterization of keratinase need to be done to assess the industrial application of the isolate.

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Isolation and Identification of Keratin Degrading (keratinolytic) Bacteria from Poultry Feather Dumping Sites

Bach E, Lopes CF, Brandelli A. 2015. Biodegradation of keratin by Gram Negative bacteria. International Biodeterioration & Biodegradation 104, 136 – 141.

Balaji S, Kumar MS, Karthikeyan R, Kumar R, Kirubanandan S, Sridhar R, Sehgal PK. 2008. Purification and characterization of an extracellular keratinase from a hornmealdegrading Bacillus subtilis MTCC 9102. World Journal of Microbiology & Biotechnology 24, 2741 – 2745.

Brandelli A, Daroit DJ, Riffel A. 2010. Biochemical features of microbial keratinases and their production and applications. Applied Microbiology and Biotechnology 85, 1735 – 1750.

Cedrola SM, de Melo AC, Mazotto AM, Lins U, Zingali RB, Rosado AS, Peixoto RS, Vermelho AB. 2012. Keratinases and sulfide from Bacillus subtilis SLC to recycle feather waste. World Journal of Microbiology & Biotechnology 28, 1259 – 1269.

Cheng SW, Hu HM, Shen SW, Takagi A, Asano M, Tsai YC. 1995. Production and characterization of keratinase of feather-degrading Bacillus licheniformis PWD-1. Bioscience Biotechnology and Biochemistry 59, 2239 – 2243.

Chitte RR, Nalawade VK, Dey S. 1999. Keratinolytic activity from the broth of a feather‐degrading thermophilic Streptomyces thermoviolaceus strain SD8. Letters in Applied Microbiology 28, 131 – 136.

Cortezi M, Cilli EM, Contiero J. 2008. Bacillus Amyloliquefaciens, A new Keratinolytic Feather-degrading Bacteria. Current Trends in Biotechnology and Pharmacy 2, 170 – 177.

Dorner W. 1926. Un procede simple pour la colouration des spores. Le. Lait. 6, 8 – 12.

Friedrich AB, Antranikian G. 1996. Keratin degradation by Fervidobacterium pennavorans, a novel thermophilic anaerobic species of the order Thermatogales. Applied and Environmental Microbiology 61, 3705 – 3710.

Gessesse A, Hatti-Kaul R, Gashe BA, Mattiasson B. 2003. Novel alkaline proteases from alkaliphilic bacteria grown on chicken feather. Enzyme and Microbial Technology 32, 519 – 524.

Giongo JL, Lucas FS, Casarin F, Heeb P, Brandelli A. 2007. Keratinolytic proteases of Bacillus species isolated from the Amazon basin showing remarkable de-hairing activity. World Journal of Microbiology & Biotechnology 23, 375 – 382.

Gouy M, Guindon S, Gascuel O. 2010. SeaView version 4, a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Molecular Biology and Evolution 27, 221 – 224.

Gradisar H, Friedrich J, Jerala R. 2005. Similarities and specificities of fungal keratinolytic proteases, comparision of keratinase of Paecilomyces marquandii and Doratomycesmicrosporus to some known proteases. Applied and Environmental Microbiology 717, 3420 – 3426.

Gradisar H, Kern S, Friedrich J. 2000. Keratinase of Doratomyces. Applied Microbiology and Biotechnology 53,196 – 200.

Gupta R, Ramnani P. 2006. Microbial keratinases and their prospective applications, an overview. Applied Microbiology and Biotechnology 70, 21 – 33.

Gupta R, Sharma R, Beg QK. 2012. Revisiting microbial keratinases, next generation proteases for sustainable biotechnology. Critical Reviews in Biotechnology 30, 1 – 13.

Gushterova A, Vasileva-Tonkova E, Dimova E, Nedkov P, Haertle T. 2005. Keratinase production by newly isolated Antarctic actinomycete strains. World Journal of Microbiology & Biotechnology 21, 831 – 834.

Kaul S, Sambali G. 1999. Production of extracellular keratinases by keratinophilic fungal species inhabiting feathers of living poultry birds Gallus domesticus, a comparison. Mycopathologia 146, 19 – 24.

Kim JM, Lim WJ, Suh HJ. 2001. Feather-degrading Bacillus species from poultry waste. Process Biochemistry 37, 287 – 291.

Kornillowicz-Kowalska T, Bohacz J. 2011. Biodegradation of keratin waste, theory and practical aspects. Waste Management 31, 1689 – 1701.

Laba W, Choinska A, Rodziewicz A. 2013. The release of sulfur compounds during degradation of feather keratin by two Bacillus strains. Acta Scientiarum Polonorum Biotechnologia 12, 29 – 40.

Lasekan A, Bakar FA, Hashim D. 2013. Potential of chicken by-products as sources of biological resources. Waste Management 33, 552 – 565.

Mabrouk MEM. 2008. Feather degradation by a new keratinolytic Streptomyces sp. MS-2. World Journal of Microbiology & Biotechnology 24, 2331 – 2338.

Mitsuiki S, Ichikawa M, Oka T, Sakai M, Moriyama Y, Sameshima Y, Goto M, Furukawa K. 2004. Molecular characterization of a keratinolytic enzyme from an alkaliphilic Nocardiopsis sp. TOA-1. Enzyme and Microbial Technology 34, 482 – 489.

Nam GW, Lee DW, Lee HS, Lee NJ, Kim BJ, Choe EA. 2002. Native feather degradation by Fervidobacterium islandicum AW-1, a newly isolating keratinase-producing thermophilic anaerobe. Archives of Microbiology 178, 538 – 547.

Nashy EHA, Ahmady AM. 2012. Keratinolytic activity of Aspergillus nodulans on dehairing of ovine hide. Egyptian Journal of Biomedical Sciences 23, 03.

Pandian S, Sundaram J, Panchatcharam P. 2012. Isolation, identification and characterization of feather degrading bacteria. European Journal of Experimental Biology 2, 274 – 282.

Parry DAD, North ACT. 1998. Hard α-keratin intermediate filament chains, substructure of the N-and C-terminal domains and the predicted structure and function of the C-terminal domains of type I and type II chains. Journal of Structural Biology 122, 67 – 75.

Paul T, Das A, Mandal A, Jana A, Maity C, Adak A, Halder SK, DasMohapatra PK, Pati BR, Mondal KC. 2014. Effective dehairing properties of keratinase from Paenibacillus woosongensis TKB2 obtained under solid state fermentation. Waste and Biomass Valorization 5, 97 – 107.

Ramnani P, Gupta R. 2004. Optimization of medium composition for keratinase production by Bacillus licheniformis RG1 using statistical methods involving response surface methodology. Biotechnology and Applied Biochemistry 40, 191-196.

Riessen S, Antranikian G. 2001. Isolation of Thermoanaerobacter keratinophilus sp. nov. a novel thermophilic, anaerobic bacterium with keratinolytic activity. Extremophiles 5, 399 – 408.

Riffel A, Daroit DJ, Brandelli A. 2011. Nutritional regulation of protease production by the feather-degrading bacterium Chryseobacterium sp. kr6. New Biotechnology 28, 153 – 157.

Riffel A, Lucas F, Heeb P, Brandelli A. 2003. Characterization of a new keratinolytic bacterium that completely degrades native feather keratin. Archives of Microbiology 179, 258 – 265.

Saibabu V, Niyongabo NF, More SS. 2013. Isolation partial purification and characterization of keratinase from Bacillus megaterium. International Research Journal of Biological Sciences 2, 13 – 20.

Shivakumar N, Raveendran S. 2015. Keratin degradation by bacteria and fungi isolated from a poultry farm and plumage. British Poultry Science 56, 210 -217.

Tapia DMT, Simoes MLG. 2008. Production and partial characterization of keratinase produced by a microorganism isolated from poultry processing plant wastewater. African Journal of Biotechnology 7, 296 – 300.

Tork S, Aly MM, Nawar L. 2008. Molecular Characterization of a New Keratinase Producing Pseudomonas sp.MS21. Journal of Genetic Engineering and Biotechnology 6, 37 – 46.

Wang JJ, Greenhut WB, Shih JC. 2005. Development of an asporogenic Bacillus licheni-formis for the production of keratinase. Journal of Applied Microbiology 98, 761 – 7.

Wawrzkiewicz K,  Lobarzewski  J,  Wolski  T. 1987. Intracellular keratinase of Trichophyton gallinae . Journal of Medical and Veterinary Mycology 25, 261- 268.

Werlang PO, Brandelli A. 2005. Characterization of a novel feather-degrading Bacillus sp. strain. Applied Biochemistry and Biotechnology 120, 71-80.

Williams CM, Richter CS, Mac, Kenzie JR, Shih JCH. 1990. Isolation, identification and characterization of a feather-degrading bacterium. Applied and Environmental Microbiology 56, 1509 – 1515.

Yamamura S, Morita Y, Hasan Q, Rao SR, Murakami Y, Yokoyama K, Tamiya E. 2002. Characterization of a new keratin-degrading bacterifum isolated from deer fur. Journal of Bioscience and Bioengineering 93, 595 – 600.

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