Welcome to International Network for Natural Sciences | INNSpub

Isolation and identification of klebsiella pneumonia causal-agent of pneumoniae from urine of childrens in hospitals of Quetta city

Research Paper | October 1, 2016

| Download 8

Deedar Ahmad Khan, Muhammd Kamran Taj, Fazal Ur Rehman, Muhammd Zahid Mustafa, Imran Taj, Ghulam Muhammad, Syed Nasrullah Agha, Zahoor Ahmad, Aziz Ullah, Shabir Ahmed

Key Words:

J. Bio. Env. Sci.9( 4), 207-212, October 2016


JBES 2016 [Generate Certificate]


The pneumoniae is worldwide leading factor which cause mortality in children however, in under developing countries the burden of klebsiella pneumoniae is greater in under 5 year age children. Therefore proper and continuously surveillance and appropriate screening tests is necessary for the detection. The samples were collected from children up to 5 years of age affected with pneumoniae and showing main symptoms of pneumoniae. The colonies with morphology suggestive of klebsiella pneumoniae were further confirmed by mac Conkey agar, eosine methylene blue agar, gram staining, different biochemical tests and PCR. Urine samples were analyzed by different techniques in which about 24% samples were positive with klebsiella pneumoniae and 76% were negative. Where sex wise ratio among positive cases has been 11% cases were from female and 13% samples were positive from males. Where age wise distributed as 4% up to six months of age, 5% from six months upto one year, 7% from one year to two year, 6% in three years, 1% in four year and 1% in five year of age. Mostly all used drugs were affective against klebsiella pneumoniae but Sulfhamethoxazole showed (25mm) and Trimethoprim showed (22mm) zone of inhibition whereas some drugs found to be resistant against klebsiella pneumoniae which were Amoxicillin, Vancomycine, Lincomycin, Penicillin, Bacitaricin, Metronedazole and Erythromycin. While confirmation through PCR samples shown clear bands of 176bp of rcs A gene. The parents should have awareness about pneumoniae to protect children from such infectious disease.


Copyright © 2016
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Isolation and identification of klebsiella pneumonia causal-agent of pneumoniae from urine of childrens in hospitals of Quetta city

Ariffin H, Ariffin W, Peng LH, Parasakthi N. 1997. Septicaemia in pediatric cancer patients: a 5-year surveillance study in University Hospital, Kuala Lumpur, Malaysia. Journal of tropical pediatrics 43(5), 279-281.

Bagley S. 1985. Habitat association of  Klebsiella  species. Infect Control 6(2), 52-8.

Bang AT, Bang RA, Tale O, Sontakke P, Solanki J, Wargantiwar R, Kelzarkar P. 1990. Reduction in pneumonia mortality and total childhood mortality by means of community-based intervention trial in Gadchiroli India. The Lancet  336(8709), 201-206.

Barnett ED, Klein JO. 2001. Bacterial infections of the respiratory tract. Infectious diseases of the fetus and newborn infant 297-317.

Black RE, Morris SS, Bryce J. 2003. Where and why are 10 million children dying every year? The lancet 361(9376), 2226-2234.

Bradford PA, Urban C, Mariano N, Projan SJ, Rahal JJ, Bush K. 1997. Imipenem resistance in Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated Amp C beta-lactamase, and the foss of an outer membrane protein. Antimicrobial agents and chemotherapy  41(3), 563-569.

Brisse S, Francine G, Grimont PAD. 2006. The Genus Klebsiella. Prokaryotes 6,159-196.

Chetoui H, Delhalle E, Melin P, Sabri A, Thonart P, De Mol P. 1999. Epidemiological typing of extended spectrum β-lactamase-producing Klebsiella pneumoniae isolates by pulsed-field gel electrophoresis and antibiotic susceptibility patterns. Research in microbiology 150(4), 265-272.

Foy HM, Cooney MK, Allan I. Kenny GE. 1979. Rates of pneumonia during influenza epidemics in Seattle, 1964 to 1975. Jama 241(3), 253-258.

Hyder AA, Wali SA, McGuckin J. 2003. The burden of disease from neonatal mortality: A review of South Asia and sub-Saharan Africa. BJOG: An International Journal of Obstetrics & Gynecology  110(10), 894-901.

Jamil I, Zafar A, Qamar MU, Ajaz H, Akhtar J, Waheed A. 2014. Multi-drug resistant Klebsiella pneumonia causing urinary tract infections in children in Pakistan. African Journal of Microbiology Research 8(4), 316-319.

Johnson WBR, Abdulkarim AA. 2013. African journal of respiratory medicine 8(2), 4.

Jokinen C, Heiskanen L, Juvonen H, Kallinen S, Karkola K, Korppi M, Kurki S, Rönnberg PR, Seppa A, Soimakallio S, Stén M. 1993. Incidence of community acquired pneumonia in the population of four municipalities in eastern Finland. American Journal of Epidemiology 137(9), 977-988.

Khan E, Ejaz M, Zafar A, Jabeen K, Shakoor S, Inayat R, Hasan R. 2010. Increased isolation of ESBL producing Klebsiella pneumonia with emergence of carbapenem resistant isolates in Pakistan: Report from a tertiary care hospital. Journal of the Pakistan Medical Association 60(3), 186.

Lawn JE. 2004. Why are 4 million newborn babies dying each year Lancet 364, 399-401.

Liu PYF, Tung JC, Ke SC, Chen SL. 1998. Molecular epidemiology of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates in a district hospital in Taiwan. Journal of clinical microbiology 36(9), pp. 2759-2762.

Macrae MB, Shannon KP, Rayner DM, Kaiser AM, Hoffman PN, French GL. 2001. A simultaneous outbreak on a neonatal unit of two strains of multiply antibiotic resistant Klebsiella pneumonia controllable only by ward closure. Journal of Hospital Infection 49(3), 183-192.

Podschun, R. Ullman U. 1998. Klebsiella spp as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin Microbial Rev 11(4), 589-603.

Qazi SA, Stoll BJ. 2009. Neonatal sepsis: a major global public health challenge. The Pediatric infectious disease journal 28(1), S1-S2.

Riley I, Carrad E, Gratten H, Gratten M, Lovuru K, Philipps P, Pratt D, Rose A, Siwi H, Smith D, Barker J. 1983. The Status of Research on Acute Respiratory-Infections in Children in Papua-New-Guinea. Pediatric Research 17(12), 1041-1043.

Selwyn BJ. 1990. The epidemiology of acute respiratory tract infection in young children: comparison of findings from several developing countries. Review of Infectious Diseases, 12 (Supplement 8), 870-888.

Skogberg K, Lyytikainen O, Ruutu P, Ollgren J, Nuorti JP. 2008. Increase in bloodstream infections in Finland, 1995-2002. Epidemiology and infection 136(01), 108-114.

Struve C, Krogfelt KA. 2004. Pathogenic potential of environmental Klebsiella pneumoniae isolates. Environmental microbiology 6(6), 584-590.

Vernet V, Philippon A, Madoulet C, Vistelle R, Jaussaud R, Chippaux C. 1995. Virulence factors (Aerobactin and mucoid phenotype) in Klebsiella pneumoniae and Escherichia coli blood culture isolates. FEMS microbiology letters 130(1), 51-57.