Isolation and identification of lipase producing bacteria from coal mines of Darra Adam Khel, Khyber Pakhtoonkhwa, Pakistan
Paper Details
Isolation and identification of lipase producing bacteria from coal mines of Darra Adam Khel, Khyber Pakhtoonkhwa, Pakistan
Abstract
Lipase producing bacteria are widespread in nature and play a key role in our daily lives. These bacteria have the capability to produce lipase enzyme which have many industrial applications. Among the various lipases, bacterial are the most significant as compared to animal and fungal lipases. The aim of current study was to isolate and to identify lipase producing bacteria. Different coal samples were collected from different areas of Darraa Adam Khel Kohat, Khyber Pakhtunkhwa, Pakistan. A total of 14 isolates were recovered from these coal samples. Samples were serially diluted and were cultured for further processing. Identification of lipolytic isolates was carried out on 1 percent tri-butarine added into the Luria Brutenia (LB) medium. Lipase producing was identified having clear zones around their colonies. Identified isolates were subjected to the gram staining and biochemical test like Oxidase Test, Catalase Test, Motility Indol Ureas (MIU) Test, Triple sugar iron (TSI) Test and Simmon Citrate Test.
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