Isolation and identification of Trichoderma species, followed by the optimization of L-glutaminase enzyme production
Paper Details
Isolation and identification of Trichoderma species, followed by the optimization of L-glutaminase enzyme production
Abstract
Advanced biotechnological techniques offer practical solutions to challenges related to food safety. To maintain quality control in food production and processing, the use of enzymes, antibodies, and microbes is indispensable. Isolation and identification of Trichoderma sp. from sugarcane soil is used for production of L-glutaminase enzyme in this study. The Trichoderma sp. was grown in PDA agar and then the species were isolated for huge production of L-glutaminase enzyme. The optimization of enzyme production was high in particular pH 7, temperature 30°C, Nacl concentration at 2g, 5g of substrate and ammonium nitrate shows higher enzyme production at 1g. Based on the analysis the enzymes have an average molecular weight around 14 to 20 kDa. By leveraging biotechnology, these manufacturing systems are being optimized leading to the faster, more efficient and flexible production of new or improved products.
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