Isolation and physiological characterization of effective antagonistic rhizobacteria from plant rhizospheric soil of Helianthus annuus
Paper Details
Isolation and physiological characterization of effective antagonistic rhizobacteria from plant rhizospheric soil of Helianthus annuus
Abstract
Diverse chemical pesticides/fungicides are used in particular doses for plant development purpose in agriculture. But for numerous side effects of chemical pesticides there is a better concern on the mutual activities between plants and the rhizospheric microorganisms. So, recently the Plant Growth Promoting Rhizobacteria (PGPR) application opening a new opportunity to resolve these issues. The rhizobacteria not only can decrease the disease incidence, but also increase the plant growth. In this article we are going to focus on collection of soil samples, isolation of bacteria, colony morphology, cell morphology and Gram’s reaction of bacterial isolates, isolated from the rhizosphere, rhizoplane and endorhizoplane of sunflower. Mainly isolates of sunflower were flat elevation, colony margins were observed from entire to erose. Most of the isolates showed opaque to translucent opacity, but one isolate NASF18 was transparent opacity. The colony color of isolates varied from yellow to milky white. From isolated PGPR strains twenty six were gram -ve bacteria and showed pink color during gram staining reaction, whereas isolates NASF2, NASF6, NASF8, NASF9, NASF17, NASF20, NASF22, NASF24, NASF26, NASF26, NASF35, NASF38 were gram +ve and showed purple color under microscope. All isolates were examined microscopically and different cell shapes were observed from Bacilli to Coccus and cell grouping of the bacterial isolates varied from streptococcus to bacillus.
Adesemoye AO, Torbert HA, Kloepper JW. 2009. Plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers. Microbial Ecology 58, 921–9.
An Y, Kang S, Kim KD, Hwang BK, Jeun Y. 2010. Enhanced defense responses of tomato plants against late blight pathogen Phytophthora infestans by pre-inoculation with rhizobacteria. Crop Protection 29, 1406–12.
Aneja KR. 2002. Experiments in Microbiology, Plant Pathology, Tissue culture and role in biocontrol by pseudomonas bacteria. New Phytologist 157, 503-523.
Arshad M, Frankenberger WT. 1998. Plant growth regulating substances in the rhizosphere: microbial production and functions. Advances in Agronomy 62, 46-151.
Berendsen RL, Pieterse CM, Bakker PAHM. 2012. The rhizosphere microbiome and plant health. Trends in Plant Science 17, 478-486.
Bloemberg GV, Lugtenberg BJJ. 2001. Molecular basis of plant growth promotion and biocontrol by rhizobacteria. Current Opinion in Plant Biology 4, 343-350.
Bochner BR. 2009. Global phenotypic characterization of bacteria. FEMS Microbiology Reviews 33, 191–205.
Cazorla FM, Duckett SB, Bergstrom ET, Noreen S, Odijk R, Lugtenberg BJJ, Thomas-Oates J, Bloemberg GV. 2006. Biocontrol of avocado dematophora root rot by antagonistic Pseudomonas fluorescens PCL1606 correlates with the production of 2-hexyl 5-propyl resorcinol. Molecular Plant-Microbe Interactions 19, 418–428.
Diby P, Sarma YR. 2006. Plant growth promoting rhizhobacteria (PGPR)-mediated root proliferation in black pepper (Piper nigrum L.) as evidenced through GS Root software. Archives of Phytopathology and Plant Protection 39, 311–4.
Dinesh R, Anandaraj M, Kumar A, Srinivasan V, Bini YK, Subila KP. 2013. Effects of plant growth promoting rhizobacteria and NPK fertilizers on biochemical and microbial properties of soils under ginger (Zingiber officinale Rosc.) cultivation. Agricultural Research 2, 346–53.
Hayat R, Ali S, Amara U, Khalid R, Ahmed I. 2010. Soil beneficial bacteria and their role in plant growth promotion: a review. Annals of Microbiology 60, 579-598.
Kavino M, Harish S, Kumar N, Saravanakumar D, Samiyappan R. 2010. Effect of chitinolytic PGPR on growth, yield and physiological attributes of banana (Musa spp.) under field conditions. Applied Soil Ecology 45, 71–7.
Kurabachew H, Wydra K. 2013. Characterization of plant growth promoting rhizobacteria and their potential as bioprotectant against tomato bacterial wilt caused by Ralstonia solanacearum. Biological Control 67, 75–83.
Majeed A, Abbasi MK, Hameed S, Imran A, Rahim N. 2015. Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion. Frontiers in Microbiology 6,198.
Manivannan M, Ganesh P, Kumar RS, Tharmaraj K, Ramya BS. 2012. Isolation, screening, characterization and antagonism assay of PGPR isolates from rhizosphere of rice plants in Cuddalore district. International Journal of Pharmaceutical and Biological Archive 3,179–185.
Mishra RK, Prakash O, Alam M, Dikshit A. 2010. Influence of Plant Growth Promoting Rhizobacteria (PGPR) on the productivity of Pelargonium graveolens L. Recent Research in Science and Technology 2, 53-57.
Rangarajan S, Saleena LM, Vasudevan P, Nair S. 2003. Biological suppression of rice disease by Pseudomonas spp. under saline soil conditions. Plant and Soil 251, 73-82.
Shaharoona B, Arshad M, Zahir ZA, Khalid A. 2006. Performance of Pseudomonas spp. containing ACC-deaminase for improving growth and yield of maize (Zea mays L.) in the presence of nitrogenous fertilizer. Soil Biology and Biochemistory 38, 2971–5.
Sturz AV, Nowak J. 2000. Endophytic communities of rhizobacteria and the strategies required to create yield enhancing associations with crops. Applied Soil Ecology 15, 183-190.
Tank N, Saraf M. 2010. Salinity-resistant plant growth promoting rhizobacteria ameliorates sodium chloride stress on tomato plants. Journal of Plant Interactions 5, 51–58.
Tien T, Gaskins M, Hubbell D. 1979. Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Pennisetum americanum L.). Applied and Environmental Microbiology 37, 1016–1024.
Vincent JM. 1970. A manual for the practical study of root nodule bacteria. Blackwell Scientific Publications. Oxford. USA. 164.
Weller DM, Raaijmakers JM, McSpadden BB, Gardener B, Thomashow LS. 2002. Microbial populations responsible for specific soil suppressiveness to plant pathogens. Annual Review of Phytopathology 40, 309–348.
Nasir Abbas, Anjum Munir, Tariq Sultan, Shahzad Asad, 2017. Isolation and physiological characterization of effective antagonistic rhizobacteria from plant rhizospheric soil of Helianthus annuus. Int. J. Biosci., 11(2), 82-90.
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