Isolation and physiological characterization of effective antagonistic rhizobacteria from plant rhizospheric soil of Helianthus annuus

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Research Paper 01/08/2017
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Isolation and physiological characterization of effective antagonistic rhizobacteria from plant rhizospheric soil of Helianthus annuus

Nasir Abbas, Anjum Munir, Tariq Sultan, Shahzad Asad
Int. J. Biosci.11( 2), 82-90, August 2017.
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Diverse chemical pesticides/fungicides are used in particular doses for plant development purpose in agriculture. But for numerous side effects of chemical pesticides there is a better concern on the mutual activities between plants and the rhizospheric microorganisms. So, recently the Plant Growth Promoting Rhizobacteria (PGPR) application opening a new opportunity to resolve these issues. The rhizobacteria not only can decrease the disease incidence, but also increase the plant growth. In this article we are going to focus on collection of soil samples, isolation of bacteria, colony morphology, cell morphology and Gram’s reaction of bacterial isolates, isolated from the rhizosphere, rhizoplane and endorhizoplane of sunflower. Mainly isolates of sunflower were flat elevation, colony margins were observed from entire to erose. Most of the isolates showed opaque to translucent opacity, but one isolate NASF18 was transparent opacity. The colony color of isolates varied from yellow to milky white. From isolated PGPR strains twenty six were gram -ve  bacteria and showed pink color during gram staining reaction, whereas isolates NASF2, NASF6, NASF8, NASF9, NASF17, NASF20, NASF22, NASF24, NASF26, NASF26, NASF35, NASF38 were gram +ve and showed purple color under microscope. All isolates were examined microscopically and different cell shapes were observed from Bacilli to Coccus and cell grouping of the bacterial isolates varied from streptococcus to bacillus.


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