Isolation and purification of allelochemicals from Cephalaria syriaca plant

Paper Details

Research Paper 01/07/2012
Views (308) Download (6)
current_issue_feature_image
publication_file

Isolation and purification of allelochemicals from Cephalaria syriaca plant

Kawa Abdulkareem Ali, Faisal Qadir Sakri, Qing X. Li
Int. J. Biosci.2( 7), 90-103, July 2012.
Certificate: IJB 2012 [Generate Certificate]

Abstract

This study was conducted to investigate the existence of allelochemicals in Cephalaria syriaca endemic weed plants, the process of purification and isolation of allelochemicals started from the plants root, shoot, seeds 70% methanolic extracts then it passed series of solvent solvent extractions with indicating the inhibitory effect of each fraction. Finally the 100% active fraction that caused 100% germination inhibition were HPLC system was employed for indicating the allelochemicals in the three studies plant parts and results indicated ten phenolic compounds which were, gallic acid, P-hydroxy benzoic acid, protocatechuic acid, Vanillic acid, Syringic acid, Sinapic acid, phluroglucinol, Chlorogenic acid, Xanthotoxine, and Chlorocatechol. Eight of these compounds were identified for the first time beside 27 unknown compounds. These results indicated the importance of that plant not only as medicinal plant but also as an alternative of herbicides for controlling weed plants.

VIEWS 17

Al saadawi IS, ZwainK H, Shahata HA. 1998.Allelopathic inhibition of growth of rice by wheat residues. Allelopathy Journal 5, 163-169.

Ali KA. 2001. Some Ecological studies on Interference between soft wheat (Triticumaestivum) and Syrian Cephalaria (Cephalariasyriaca). M.Sc. Thesis in Biology.zaqCollege of Education.University of Salahaddin. Iraq.

Ali KA, Aziz FH. 2002. Study the effect of root and shoot extracts of Syrian cephalaria (Cephalariasyriaca) on wheat seeds (Triticum aestivum) germination properties. Zanco. 14(2), 15-24.

Al-Mezori HA, Al-SaadawiI S, Al-HadithiT R. 1999. Allelopathic effects of corn residues on the sub sequent corn crop.Allelopathy Journal 6, 193– 200.

Anaya AL. 2006.Allelopathic organisms and molecules promising bioregulators for the control of plant diseases, weeds, and other pests.Allelochemicals: Biological Control of Plant Pathogens and Diseases. Springer. New York. USA.

BohmA B. 1998. Introduction to Flavonoids.Harwood academic publishers. Amesterdam. Netherland.

Chen H AT.1997. Medicinal plant resources of Dipsacaceae in China.Zhongguozhong Yao ZaZhi. 22(11), 649-652.

ChernushevichI V, Loboda A V, Thomson B A. 2001. An introduction to quadrupole-time- of-flight mass spectrometry.Journal of Mass spectrometry 36, 849- 865.

Chou CH. 1998. Adaptive autointoxication mechanisms in rice.Allelopathy in rice.Edited by M. Olofsdotter.Manila . Philippines.

Cia W. 1997.Comparative studies of allelopathy and allelochemicals of some common weeds in cyperaceae.Plant physiology PhD dissertation. University of Hawaii. USA.

CollJ C, Bruce FB. 1986. The Application of Vacuum Chromatography to the Separation of Terpene Mixtures. Joumal of Natural Products. 49(5), 934-936.

Cooke M, Poole CF. 2000. Encyclopedia of separation science.Academic press. UK.

Dayan EF, RomagniJ G, Stephen OD. 2000.Investigating the mode of action of natural phytotoxins.Journal of Chemical Ecology 26(9), 2079- 2094.

EinhelligF A. 2004.Mode of allelochemical action of phenolic compounds.Allelopathy: chemistry and mode of action of allelochemicals / edited by Francisco A. Macias, Juan C.G. Galindo, José M.G. Molinillo, and Horace G. Cutler. USA.

FifieldF W, Kealey D. 2000. Principles and Practice of Analytical Chemistry.Blackwell Science Ltd. 5th edition. Japan.

GafoorA O. 2002. Allelopathical interaction between some common weed plants and winter wheat Triticumaestivum L. cv. ARAS.Ph D. Dissertation.College of Agriculture .University of Dohuk. Iraq.

Galiskan OA, Huseyin A. 1995. A BidesmosidicTriterpeneSaponin from Cephalariatransslvanica. Phytochemistry 38(6), 1493-1495.

Godjevac D, Vlatka V, Nebojsa M, Vele T, Ped A, Slobodan M. 2004. Flavonoids from flowers of Cephalariapastricensis and their antiradical activity. Journal serbian chemical society. 69(11), 883-886.

Godjevac D, Vlatka V, NebojsaM, Vele T, PedA, SlobodanM. 2006.Triterpenoidsaponins and iridoid glycosides from the aerial parts of Cephalariapastricensis.Biochemical Systematics and Ecology 34, 890-893.

Gorge SA. 1983. Effect of different ratios of Syrian Cephalaria (Cephalariasyriaca) on taste of wheat bread.Institute of practical researches publication.Baghdada. Iraq.

Griffiths WJ, Andreas PJ, Liu S, RaiD K, Wang Y. 2001. Electrospray and tandem mass spectrometry in biochemistry. Biochemical Journal 355, 545- 561.

Harborne  JB.  1980.  Phytochemical  methods. Science, paperback.

Hess D. 1975. Plant physiology.Springer- Verlag Berlin.

Hoagland RE, Williams RD. 2004. Bioassays-useful tools for the study of allelopathy. Allelopathy: chemistry and mode of action of allelochemicals / edited by Francisco A. Macias, Juan C.G. Galindo, José M.G. Molinillo, and Horace G. Cutler. USA.

Inderjit M, DakshiniK MM. 1996. Allelopathic potential of Pluchealanceolata, Comparative studies of cultivated Fields. Weed Science 40, 393-396.

Kirmizigul S, Huseyin A. 2002. New TriterpenicSaponins from Cephalariatransslvanica. Turk J chem. 26, 947-954.

Kirmizigul S, HuseyinA. 1994. An acidic Triterpene Glycoside from Cephalariatransslvanica.Phytochemistry. 36(6), 1555-1556.

Kirmizigul S, HuseyinA, Malcolm ER. 1995.Triterpenoid Glycosides from Cephalariatransslvanica. Phytochemistry 36(5), 1171-1174.

KpoviessiD S, Fernand AG, Joachim DG, Georges CA, Mohamed H, Mansourou M, Joëlle Q. 2006. Allelopathic effects on cowpea (Vignaunguiculata (L.)Walp) plant and cytotoxic activities of sterols and triterpene isolated from Justiciaanselliana (NEES) T. Anders. Electronic Journal of Natural Substances 1, 12-19.

Kupidlowska E, DobrzynskaK, Parys E, Alicja M. 1994. Effect of coumarin and xanthotoxin on mitochondrial structure, oxygen uptake, and succinate dehydrogenase activity in onion root cells.Journal of Chemical Ecology. 20(10), 2471- 2477.

Lambers HF, Stuart C, Thijs LP. 2008. Plant physiological ecology.Springer .2nd edition. USA.

Macias AFC, Jose MG. 2000. Search for a Standard Phytotoxic Bioassay for Allelochemicals. Journal of Agricultural and Food Chemistry 48, 2512-2521.

Macias FA, Galindo JC, Diego C, Velasco RF. 1999. Sesquiterpene lactones with potential use as natural herbicide models (1): trans,trans-Germaacranolides. Journal of Agricultural and Food Chemistry 47, 4407- 4414.

Macias FA, Molinillo JM, Varela RM, Galindo JC. 2007. Allelopathy – a natural alternative for weed control. Pest Management Science 63(4), 327- 348.

Moreiras-sanchez AM, Weiss OA, Reigosa-roger MJ. 2004. Allelopathic evidences in the poaceae. The Botanical review 69(3), 300- 319.

Narwall SS. 2006. Allelopathy in ecological sustainable agriculture.Allelopathy a physiological process with ecological implications.Springer. New York. USA.

Pasi S, AligiannisN, Alexios-Leandros S, Ioanna BC. 2002. A new lignan glycoside and other constituents from Cephalariaambrosioides.Natural Product Research. 16(6), 365-370.

Pelletier SW, Hitesh PG, Haridutt KD. 1986. Separation of Diterpenoid Alkaloid Mixtures Using Vacuum Liquid Chromatography.Joumal of Natural Products 49(5), 892-900.

Piechowski K, Mathees DP, Resse RN, Majerle RSK. 2006.Identification of potential allelopathicisobutylamides in Echinceaangustifolia D.C. roots.Journal of Biological Science 6(6), 978-984.

Ramanathan K, Clifford HK, Krishna. NR. 2006. Alleopathy for weed control in aquatic and wetland systems.Allelochemicals: Biological Control of Plant Pathogens and Diseases. University of Delhi, Delhi, India.

Rice EL. 1984. Allelopathy 2nd Edition.Academic Press.NewYork.USA.

Rimando  AM, Olofsdotter M,  Dayan  FE, DukeS O. 2001. Searching for Rice Allelochemicals: An Example of Bioassay-Guided Isolation.Agronomy Journal 93, 16-20.

Rizvi SJH, Rizvi V. 1992. Allelopathy, basic and applied aspects.Chapman and Hall. UK.

Robson TO, Americanos PJ, Abu-irmaileh BE. 1991. Major weeds of the near east. FAO plant protection and production paper.

Sajjadi SE, Noroozi P. 2007. Isolation and identification of xanthotoxin (8-methoxypsoralen) from the fruits of HeracleumpersicumDesf.Ex Fischer.Research in Pharmaceutical Science 2, 13-16.

Sakakibara H, Honda Y, Nakagawa S, AshidaH, Kanazawa K. 2003. Simultaneous determination of all polyphenols in vegetables, fruits, and teas.Journal of Agricultural food chemistry 51, 571- 581.

Skulman BW, Matticej D, Cain MD, Gbur EE. 2004. Evidence for allelopathic interference of Japanese honeysuckle (Lonicera japonica) to loblolly and shortleaf pine regeneration. Weed Since 52(3), 433- 439.

Tabatadze N, Elias R, Faure R, DePauw-Gillet M, Kemertelidze E, Chea A, Ollivier E. 2007. Cytotoxic TriterpenoidSaponins from the Roots of Cephalaria gigantean. Chemical and pharmaceutical bulletin 55(1), 102-105.

Taiz L, Zeiger E. 2006. Plant physiology.Sinauer. Massachusetts. USA.

Vokou D, Chalkos D, Karamanoli K. 2006. Microorganisms and allelopathy: a one-sided approach. Allelopathy a physiological process with ecological implications.Springer. New York. USA.

Weidenhamer JD. 2006. Distinguishing allelopathy from resource competition: the role of allelopathy.Allelopathy a physiological process with ecological implications.Springer. New York. USA.

Wickenden S. 2001.The isolation and purification of Putative Allelochemicals from Chrysanthemum morifolium tissue.Thesis.University of Guelph. Canada.

Wu CM, Koehler PE, Ayres JC. 1972. Isolation and identification of xanthotoxin (8- methoxypsoralen) and bergapten (5-methoxypsoralen) from celery infected with Sclerotiniasclerotiorum. Applied Microbiology. May: 852- 856.

Zahida I, Golisz A, Furubayashi A, Habib N, FujiiYoshiharu. 2005. Allelopathic potential of buckwheat. Fourth World Congress in Allelopathy. Charles Sturt University (CSU), WaggaWagga, NSW Australia from 21 – 26 August 2005.

Zeng RS. 2008. Allelopathy in Chinese Ancient and Modern Agriculture.Allelopathy in Sustainable Agriculture and Forestry.Springer. USA.

Zhou YH, Yu QJ. 2006. Allelochemicals and photosynthesis.Allelopathy: a physiological process with ecological implications. Springer. Netherlands: 127-139.