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Isolation and Resistance Pattern of Bacteria from Infected Corneal Ulcer Patients in Human

Md. Anamul Haq, Mst. Mahbubay Habibah, Md. Rayhan Chowdhury, Md. Ariful Haque

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Int. J. Biosci.19(3), 83-89, September 2021

DOI: http://dx.doi.org/10.12692/ijb/19.3.83-89


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A corneal ulcer is an infectious or more severely infective disease of the cornea, which affects the epithelial surface of the corneal stroma after trachoma. Corneal ulcers are the world’s second most important cause of blindness. This research was performed to determine the microbiological composition of corneal ulcers and the resistant pattern of bacteria collected from the patients of corneal ulcers from the department of ophthalmology at Rajshahi medical college hospital. The isolated bacteria were gram-negative. In Klebsiella selective agar, it showed a purple-magenta-colored colony, as well as in ESBL selective agar; the isolated bacteria showed a purple or blue-colored colony. Thus the isolate has been identified as Klebsiella pneumoniae by selective agar test. Eight different antibiotics were used to evaluate the isolate for antibiotic sensitivity using the disc diffusion method. The isolated bacteria showed resistance to three of the antibiotics, namely ampicillin, vancomycin, and bacitracin, and showed susceptibility to five such as gentamicin, amikacin, chloramphenicol, imipenem, levofloxacin. The isolated bacteria exhibited 37.5% resistance and 62.5% sensitivity against those eight antibiotics. According to the findings of this study, Klebsiella pneumoniae can be detected in corneal ulcers, and mentioned antibiotics can be used to treat these bacteria.


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Isolation and Resistance Pattern of Bacteria from Infected Corneal Ulcer Patients in Human

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