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Research Paper | August 10, 2022

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Isolation, characterization and antimicrobial screening of bioactive substance-producing actinobacteria isolated from Algerian soil samples

Amina Zergoug, Chaimaa Hammani

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Int. J. Biosci.21(2), 199-211, August 2022

DOI: http://dx.doi.org/10.12692/ijb/21.2.199-211

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Abstract

The emergence of multidrug-resistant bacteria has necessitated the search for novel bioactive compounds in unexplored and natural environments. Actinobacteria contain important bioactive substances. Actinobacteria are Gram-positive, facultatively anaerobic, filamentous, fungus-like bacteria that remain among the leading producers of natural antibiotics. Consequently, in this work, 50 actinobacteria were isolated from 5 soil samples collected from the Algerian provinces of Medea and Blida in searching for untapped producers of antimicrobial compounds. All the isolates were further subjected to antimicrobial screening against pathogenic bacteria.The obtained results indicated that sixteen of the isolates showed antimicrobial activities against most of the tested pathogenic microorganisms. Therefore, these promising isolates, previously identified as Streptomyces by morphological, biochemical and physiological methods, were selected for antibiotics susceptibility, kinetic growth production of antibiotics, evaluation of pH and metabolite production such as biomolecules.These results pointed out that actinobacteria from Algerian provinces of Medea and Blida soils suggest that these selected isolates could be excellent candidates,especially BN01, BN20, BN17, CH2,OUZ 5 and TAM 47,for the discovery of antibiotics and could be potential sources of antimicrobial bioactive compounds. For antibiotics susceptibility,all the isolates showed a sensitivity of 100% to Amoxicillin, Neomycin and Chlortetracycline. After fermentation in ISP2 medium an extraction was done, the results revealed that the maximum zone of inhibition was recorded against all the tested pathogens.This finding may be significant for future research aimed at developing broad-spectrum antibiotics for therapeutic purposes.

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Isolation, characterization and antimicrobial screening of bioactive substance-producing actinobacteria isolated from Algerian soil samples

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