Isolation, characterization and antimicrobial screening of bioactive substance-producing actinobacteria isolated from Algerian soil samples

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Research Paper 10/08/2022
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Isolation, characterization and antimicrobial screening of bioactive substance-producing actinobacteria isolated from Algerian soil samples

Amina Zergoug, Chaimaa Hammani
Int. J. Biosci.21( 2), 199-211, August 2022.
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Abstract

The emergence of multidrug-resistant bacteria has necessitated the search for novel bioactive compounds in unexplored and natural environments. Actinobacteria contain important bioactive substances. Actinobacteria are Gram-positive, facultatively anaerobic, filamentous, fungus-like bacteria that remain among the leading producers of natural antibiotics. Consequently, in this work, 50 actinobacteria were isolated from 5 soil samples collected from the Algerian provinces of Medea and Blida in searching for untapped producers of antimicrobial compounds. All the isolates were further subjected to antimicrobial screening against pathogenic bacteria.The obtained results indicated that sixteen of the isolates showed antimicrobial activities against most of the tested pathogenic microorganisms. Therefore, these promising isolates, previously identified as Streptomyces by morphological, biochemical and physiological methods, were selected for antibiotics susceptibility, kinetic growth production of antibiotics, evaluation of pH and metabolite production such as biomolecules.These results pointed out that actinobacteria from Algerian provinces of Medea and Blida soils suggest that these selected isolates could be excellent candidates,especially BN01, BN20, BN17, CH2,OUZ 5 and TAM 47,for the discovery of antibiotics and could be potential sources of antimicrobial bioactive compounds. For antibiotics susceptibility,all the isolates showed a sensitivity of 100% to Amoxicillin, Neomycin and Chlortetracycline. After fermentation in ISP2 medium an extraction was done, the results revealed that the maximum zone of inhibition was recorded against all the tested pathogens.This finding may be significant for future research aimed at developing broad-spectrum antibiotics for therapeutic purposes.

VIEWS 164

Alharbi SA, Arunachalam C, Murugan AM, Wainwright M. 2012. Antibacterial activity of actinomycetes isolated from terrestrial soil of Saudia Arabia. Journal of food, agriculture and environment. 10(2), 1093-1097.

Audrey W.  2007. Disk Diffusion Test and Gradient Methodologies. In: Schwalbe R, Steele-Moore L, Goodwin AC, editors. Antimicrobial Susceptibility Testing Protocols: Boca Raton: CRC Press Taylor and Francis Group, p 53-72.

Balouiri M, Sadiki M, Koraichi Ibnsouda S. 2016. Methods for in vitro evaluating antimicrobial activity: A review, Journal of Pharmaceutical Analysis 6(2), 71–79. https://doi.org/10.1016/j.jpha.2015.11.005

Baskaran R, Vijayakumar R, Mohan P. 2011. Enrichment method for the isolation of bioactive actinomycetes from mangrove sediments of Andaman Islands, India. Malaysian Journal of Microbiology. 7(1), 26–32.  http://dx.doi.org/10.21161/mjm.24410

Berdy J. 2005. Bioactive microbial metabolites. The Journal of Antibiotics 58, 1-26. https://doi.org/10.1038/ja.2005.1

Boudemagh A. 2007. Isolation, from Saharan soils, of actinomycetal bacteria producing antifungal molecules, molecular identification of active strains. Doctoral thesis, specialty: Applied Microbiology. Mentouri University, Constantine, p 62.

Breton A, Theilleux J, Sanglier JJ, Viobis G. 1989. Producing organizations: Biology, taxonomy and ecology. In “Biotechnology of Antibiotics”. Larpent J.P. and Boar J.J., Masson: Paris, p 33-70.

Djinni I, Defant A, Djoudi W, Chaabane Chaouch F, Souagui S, Kecha M, Mancini I. 2019. Modeling improved production of the chemotherapeutic polypeptide actinomycin D by a novel Streptomyces sp. Strain from a Saharan soil. Heliyon volume 5(5), e01695. https://doi.org/10.1016/j.heliyon.2019.e01695

El-Nakeeb MA, Lechvalier HA. 1963.  Selective isolation of aerobic actinomycetes. Applied Microbiology 11(2), 75-77. https://doi.org/10.1128/am.11.2.75-77.1963

Gousterova A, Paskaleva D, Vasileva-Tonkova E. 2014. Characterization of culturable thermophilic actinobacteria from Livingston Island, Antarctica. International Research Journal of Biological Sciences 3(3), 30-36

Hadj Rabia-Boukhalfa Y, Eveno Y, Karama S.  2017. Isolation, purification and chemical characterization of a new angucyclinone compound produced by a new halotolerant Nocardiopsis sp. HR-4 strain. World Journal of Microbiology and Biotechnology 33(6), 126. https://doi.org/10.1007/s11274-017-2292-8

Jihani S,  Brodolin K,  Iraqui M,  Ibnsouda S,  Haggoud A. 2012.  Isolation and molecular identification of antibiotic- producing actinomycetes from an old house in the medina of Fez, Morocco. African Journal of Microbiology research 6(47), 7370- 7376. https://doi.org/10.5897/AJMR12.1711

Kitouni M. 2007. Isolation of producing actinomycetal bacteriaof antibiotics from extreme ecosystems molecular identification ofactive strains and preliminary characterization of the substances produced. Thesis of PhD in Microbiology: Mentouri-Constantine University.

Kumala T D, Agustiani D and Antonius S. 2017. Secondary Metabolites Production by Actinomycetes and their Antifungal Activity. ICBS Conference Proceedings International Conference on Biological Science, KnE Life Sciences, pages 256–264.

Lamari L, Zitouni A, Dob T, Sabaou N, Germain P, Lefebvre G, Seguin E, Tillequin F. 2002. New dithiolopyrrolone antibiotics from Saccharothrix sp. SA233. I. Taxonomy, production, isolation, and biological activities. The Journal of Antibiotics 55, 696–701. https://doi.org/10.7164/antibiotics.55.696

Lee LH, Zainal N, Azman AS. 2014. Diversity and antimicrobial activities of actinobacteria isolated from tropical mangrove sediments in Malaysia. Scientific World Journal, Article ID 698178, 14 pages. https://doi.org/10.1155/2014/698178

Madigan MT, Martinko JM. 2007. Biologie des microorganismes. Pearson Education France, 11ème edition, p331-423, 686-718.

Martin JF, Mcdaniel LE. 1975. Kinetics of biosynthesis of polyene macrolide antibiotics in batch cultures: Cell maturation time, Biotechnology and Bioengineering 17, 925-938. https://doi.org/10.1002/bit.260170611

Palaniyandi SA, Yang SH, Zhang L, Suh, JW. 2013. Effects of actinobacteria on plant disease suppression and growth promotion. Applied Microbiology and Biotechnology 97, 9621–9636. https://doi.org/10.1007/s00253-013-5206-1

Passari AK, Mishra VK, Singh G, Singh P, Kumar B, Gupta VK, Sarma RK, Saikia R, Donovan AO, Singh BP. 2017. Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production. Scientific Reports 7, 11809. https://doi.org/10.1038/s41598-017-12235-4

Pfefferle C, Theobald   U, Gürtler H, Fiedler HP. 2000. Improved secondary metabolite production in the genus Streptosporangium by optimization of the fermentation conditions.  Journal of Biotechnology 80(2), 135- 142. https://doi.org/10.1016/S0168-1656(00)00249-2

Praveen V, Tripathi D, Tripathi CKM, Bihari V. 2008. Nutritional regulation of actinomycin-D production by a new isolate of Streptomyces sindenensis using statistical methods. Indian Journal of Experimental Biology 46(2), 138-144.

Rai M, Bhattrai N, Dhungel N, Mandal PK. 2016. Isolation of antibiotic producing actinomycetes from soil of Kathmndu valley and assessment of their antimicrobial activities. International Journal of Microbiology and Allied Sciences 2(4), p 22–26.

Reghioua S, Boughachiche F, Oulmi L Zerizer H, Kitouni M, Boudemagh A, Boulahfrouf A. 2008. Separation and preliminary characterization of antibiotics produced by a representative strain of actinomycetes arid soil isolates in the Biskra region. Sciences & Technologie C – N° 28, p 59-64

Sateesh V, Naikpatil Rathod JL. 2011 a. Antimicrobial and Cytotoxic activity of actinomycetes from Karwar Coast, West Coast of India. World Journal of Sciences and Technology 1(1), 7-10.

Silini S. 2012. Contribution to the study of the biodegradation of methyl ethyl ketone in a bath reactor by actinomycetes isolated from activated sludge from the El Atmania wastewater treatment plant. doctorat’s thesis in Ecology. Mentouri-Constantine University, Algeria. P 2334-39.

Singh V, Tripathi CKM. 2011. Olivanic acid production in fed batch cultivation by Streptomyces olivaceus MTCC 6820. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2(4), 726-731.

Strub C. 2008. Modeling and optimization of thiolutin production in Saccharothrix algeriensis. Doctoral thesis. University of Toulouse INPENSAT. France

Taechowisan T, Lu C, Shen Y. 2005. Secondary metabolites from endophytic Streptomyces aureofaciens CMUAc130 and their antifungal activity. Microbiology 151(5), 1691-5. https://doi.org/10.1099/mic.0.27758-0

Williams ST, Sharpe ME, Holt JG. 1989. Bergey’s manual of systematic bacteriology. Baltimore: Williams and Wilkins 4.

Zothanpuia A, Passari AK, Gupta VK, Singh BP. 2016.Detection of antibiotic-resistant bacteria endowed with antimicrobial activity from a fresh water lake and their phylogenetic affiliation. Peer J 4– e2103. https://doi.org/10.7717/peerj.2103