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Isolation, characterization and control of a fungus responsible for post-harvest mango spoilage from northern region of Bangladesh

Research Paper | November 1, 2017

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Md. Sifat Rahi, Israt Jerin, Salek Ahmed Sajib, Md. Shihabul Islam, Zannatul Chadni, Kazi Md. Faisal Hoque, Md Abu Reza

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Int. J. Biosci.11( 5), 260-269, November 2017

DOI: http://dx.doi.org/10.12692/ijb/11.5.260-269


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Mango, ‘the king of the fruits’ is one of the most popular, nutritionally rich fruit in Bangladesh. One of the limiting factors that influence the economic value of mango is its short storage life at ambient temperature due to spoilage, caused by microbial invasion. Fungi are the primary spoiling organism as they are rich in cell wall degrading enzymes. Current study was carried out to isolate, characterize and to find out a cost effective control measure for the fungal strains associated with post-harvest spoilage of mango. One fungal strain was isolated from the spoiled ‘‘Totapuri’ mango. The primary characterization of the fungal strain shows that the colony diameter, culture characteristics and sporulation of the fungus is greatly influenced by the type of growth medium used. CDA exhibited higher mycelial growth, whereas the PDA culture medium was responsible for heavy sporulation. Molecular confirmation of the isolate as fungus was done by the extraction of genomic DNA and ITS region amplification by PCR. The fungi grew optimally at 25°C, pH 9 and in dextrose carbohydrate source. The fungus exhibited high sensitivity to citric acid and NaCl.. The results of this investigation combined with further studies like ITS region sequencing, can be used in precise identification of organism causing mango spoilage and it will also be possible to decipher a biological control mechanism for this fungus.


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