Welcome to International Network for Natural Sciences | INNSpub

Isolation, Characterization of PSB stains from rock phosphate and their potential as Biofertilizer

Research Paper | January 1, 2017

| Download 42

Abdul Latif, Ghulam Jilani, Rifat Hayat, Ahmed Ali Khan, Muhammad Azeem, Muhammad Ehsan, Muhammad Umair Mubarak

Key Words:

Int. J. Biosci.10( 1), 72-80, January 2017

DOI: http://dx.doi.org/10.12692/ijb/10.1.72-80


IJB 2017 [Generate Certificate]


Rock phosphate (RP) is an important alternative source of phosphorus (P) and phosphorus solubilizing bacteria (PSB) are responsible for the efficient use of this valuable source. In vitro study was conducted in the microbiology laboratory of Soil Science PMAS-AAUR to assess the ability of PSB strains to solubilize Phosphorus from Rock Phosphate amended broth medium. For this purpose, different phosphorus solubilizing strains were first isolated from Rock Phosphate and characterized by Gram testing and API 20 E-Kit. The strains were named as Pseudomonas after characterization. These strains made halo zone around its colony, which confirmed them as P-solubilizer. Among the various P-solubilizers, best strains were picked and their efficiency was tested in the broth culture. The drop of pH and P-solubilization were the key factors to be observed. An inverse relation between drop of pH and solubilized P was noticeable. The drop of pH was due to the productions of various organic acids and was measured by pH meter while mineralization of soil P was due to acid phosphatase. The drop of pH and Phosphorus was measured by Spectrophotometer at 880 nm. The individual application of these strains showed great variation in their capability to solubilize RP and same results were seen when applied in soil. However, the co-inoculation of the strains showed significant ability to solubilize Rock Phosphate. The results of the current study confirm that PSB have great potential to be used as a bio fertilizer.


Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Isolation, Characterization of PSB stains from rock phosphate and their potential as Biofertilizer

Akhtar N, Iqbal A, Qureshi MA, Khan KH. 2010. Effect of phosphate solubilizing bacteria on the phosphorus availability and yield of cotton (Gossypium hirsutum). Journal of Scientific Research 40(1), 15-24.

Alam S, Khalil S, Ayub N, Rashid M. 2002. In vitro solubilization of inorganic phosphate by phosphate solubilizing microorganisms (PSM) from Maize rhizosphere. International Journal of Agriculture and Biology4, 454-458.

Chen YP, Rekha PD, Arun AB, Shen FT, Lai WA, Young CC. 2005. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate abilities. Applied Soil Ecology 34, 33-41. http://dx.doi.org/10.1016/j.apsoil.2005.12.002

Edi-Premono M, Moawad A, Vleck PLG. 1996. Effect of phosphate solubilizing Pseudomonas putidaon the growth of maize and its survival in the rhizosphere. Indonasian Journal of Crop Science 11, 13-23.

Fernández LA, Zalba P, Gómez MA, Sagardoy MA. 2007. Phosphate-solubilization activity of bacterial strains in soil and their effect on soybean growth under greenhouse conditions. Biology and Fertility of Soils 43, 805-809. http://dx.doi.org/10.1007/s00374-007-0172-3

Goenadi DH, Siswanto, Sugiarto Y. 2000. Bio activation of poorly soluble phosphate rock with phosphorus solubilizing fungus. Soil Science Society of America Journal 64, 927-932. http://dx.doi.org/10.2136/sssaj2000.643927x

Goldstein AH. 1986. Bacterial solubilization of mineral phosphates: historical perspective and future prospects. American Journal of Alternative Agriculture 1, 51-57.

Goldstein AH. 1994. Involvement of the quinoprotein glucose dehydrogenises in the solubilization of exogenous phosphates by gram-negative bacteria. In: A. Torriani Gorini, E. Yagil and S. Silver (Eds.), Phosphate in Microorganisms: Cellular and Molecular Biology. Washington DC, USA: ASM Press,  197-203. https://doi.org/10.1017/S0889189300000886

Hinsinger P. 2001.Bioavailability of soil inorganic P in the rhizosphere as affected by root induced chemical changes: a review. Plant Soil 237, 173-195. http://dx.doi.org/10.1023/A:1013351617532

Joseph S, Jisha MS. 2009. Buffering reduces phosphate solubilizing ability of selected strains of bacteria. World Journal of Agricultural Sciences5, 135-137.

Keneni A, Assefa F, Prabu PC. 2010. Isolation of Phosphate Solubilizing Bacteria from the Rhizosphere of Faba Bean of Ethiopia and Their Abilities on Solubilizing Insoluble Phosphates. Journal of Agricultural Science and Technology 12, 79-89.

Khan AA, Jilani G, Akhtar MS, Naqvi SSM, Rasheed M. 2009. Phosphorus Solubilizing Bacteria: Occurrence, Mechanisms and their Role in Crop Production. Journal of Agriculture and Biological Science 1(1), 48-58, 2009.

Maliha RK, Samina,Najma A, Sadia A, Farooq L. 2004. Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms under in vitro conditions. Pakistan Journal of Biological Sciences 7, 187-196.

McLean EO. 1982. Soil pH and Lime requirement. In: Page, A. L., R. H. Miller and D. R. Keeney (eds.), Methods of Soil Analysis Part 2. Madison, Wisconsin, USA: American Society of Agronomy No. 9, 199-209.

Muhammadi K. 2012. Phosphorus solubilizing Bacteria, occurrence, mechanisms and their role in crop production. Journal of Research and Environment 2(1), 80-85.

Narsian V, Takkar J, Patel HH. 1995. Mineral phosphte solubilization by Asperigillus aculeatus. Indian Journal of Experimental Biology 31, 33-91.

Olsen SR, Sommers LE. 1982. Phosphorus. In: Methods of soil analysis Part 2. Madison Wisconsin, USA:American Society of Agronomy9, 403-427.

Panhwar QA, Radziah O, Sariah M, Razi IM. 2009. Solubilization of phosphate forms by phosphate solubilizing bacteria isolated from aerobic rice. International Journal of Agriculture Biology 11, 667-673.

Patil MG, Sayyed RZ, Chaudhari AB, Chincholkar SB. 2002. Phosphate solubilizing microbes: a potential bioinoculant for efficient use of phosphate fertilizers. Scientific Publisher, Jodhpur, 107-118.

Rajan SSS, Watkinson JH, Sinclair AG. 1996. Phosphate rock of for direct application to soils. Advance Agronomy 57, 77-159. http://dx.doi.org/10.1016/S0065-2113(08)60923-2

Rashid M, Samina K, Najma A, Sadia A, Farooq L. 2004. Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms under in vitro conditions. Pakistan Journal of Biological Sciences 7, 187-196.

Seshadri S, Muthukumarasamy R, Lakshmi-narasimhan C, Ignacimuthu S. 2000. Solubilization of inorganic phosphates by Azospirillum halopraeferans. Current Science 79, 565-567.

Steel RGD, Torrie JH, Dickie DA. 1997. Principles and procedures of statistics- a biometric approach, 3rd ed. Mc Graw Hill Publishing Company, Toronto.

Smibert RM,  Kreig NR. 1994. Phenotypic characterization. In: methods for general and molecular bacteriology. Washington DC: .American Society of Microbiology, No. 12. p. 607-654. http://dx.doi.org/10.1016/S0378-4290(02)00048-5

Sundara B, Natarajan V, Hari K. 2002. Influence of phosphorus solubilizing bacteria on the changes in soil available phosphorus and sugarcane yields. Field Crops Research 77, 43-49. http://dx.doi.org/10.1016/S0378-4290(02)00048-5

Toro M. 2007. Phosphate solubilizing microorganisms in the rhizosphere of native plants from tropical savannas: An adaptive strategy to acid soils? In: Velazquez C, Rodriguez-Barrueco E, ed. Developments in Plant and Soil Sciences. The Netherlands: Springer, 249-252 p.

Wani PA, Khan MS, Zaidi A. 2007. Co-inoculation of nitrogen-fixing and phosphate solubilizing bacteria to promote growth, yield and nutrient uptake in chickpea. Acta Agronomica Hungarica 55, 315-323. http://dx.doi.org/10.1556/AAgr.55.2007.3.7

Whitelaw MA. 2000. Growth promotion of plants inoculated with phosphate solubilizing fungi. Advance Agronomy 69, 99-151. http://dx.doi.org/10.1016/S0065-2113(08)60948-7

Wollum II AG. 1982. Cultural methods for soil microorganisms. In: Page AL, Miller RH, Keeney DR, Ed. Methods of Soil Analysis: Chemical and Microbial Properties. Secondedition. Madison Wisconsin, USA:ASA and SSSA Pub. 718-802 p.