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Isolation, identification and ability of endophytic fungi in stimulating cocoa seed germination

Research Paper | January 1, 2017

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Muh Assad, Muhammad Taufik, H. S. Gusnawaty, Asniah

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Int. J. Biosci.10( 1), 270-278, January 2017

DOI: http://dx.doi.org/10.12692/ijb/10.1.270-278


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Cocoa is an important commodity of the world, with demands increasing every year. Indonesia is the third largest cocoa producer in the world and continues to increase its production. One of the factors limiting the production of cocoa is an infectious disease Vascular Streak Dieback (VSD), caused by Ceratobasidium theobromae. VSD disease can cause yield losses up to 50%, and severe infections cause the death of cocoa plants. This research was conducted using a survey method, isolation and identification of endophytic fungi based on morphological characters that were based on fungi identification book, as well as testing the ability of the fungi to stimulate germination of cacao seeds in vitro. The study was able to isolate and identify six isolates of endophytic fungi, namely Paecilomyces sp isolates EP1, EP3, EP6, and EP8, Cladosporium sp. and Nigrospora sp. Paecilomyces sp. isolate EP1 was endophytic fungus which had the ability to stimulate the germination of cacao seeds by 100%, followed by Nigrospora sp. (90%). Presumably, the endophytic fungi produced hormones to stimulate the process of cacao seed germination. One isolate of Aspergillus sp. can not be categorized as an endophytic fungus of cocoa plant. Endophytic fungus Paecilomyces sp isolate EP1 is promising to be used as an alternative environmentally friendly VSD disease control agent.


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Isolation, identification and ability of endophytic fungi in stimulating cocoa seed germination

Amin N, Salam M, Junaidi M, Aswan, Baco MS. 2014. Isolation and identification of endophytic fungi from cocoa plant resistant VSD M.05 and cocoa plant Susceptible VSD M.01in South Sulawesi, Indonesia. International Journal of Current Microbiology and Applied Science 3(2), 459-467. http://www.ijcmas.com/vol-3-2/Nur Amin, et al.pdf

Askindo. 2008. Discussion Report on VSD. Report was presented at a National Meeting ”A Day Discussion: Crass Program Addressing VSD Disease”, 5 June 2008. Clarion Hotel, Makassar.

Azhar I, Lee MT. 2004. Perspective for cocoa cultivation in Malaysia: Re-Look at the economic indicators. Malaysian Cocoa Journal 1, 1-18.

Asniah. 2009. Potential of fungi from grassroots, sedges and bamboo rootings for disease control of “Gada” clubroot disease of Broccoli. Postgraduate Thesis, Bogor Institute of Agriculture, Bogor, Indonesia.

Asniah, Lestari D, Darlian L, Mariadi. 2014. Potential of nonpathogenic endophytic fungi from pepper crops (Capsicum annuum L.) as biofungicides of pathogen Fusarium oxysporum. Jurnal Agroteknos 24(2), 208-213.

Bailey BA, Strem MD, Wood D. 2009. Trichoderma species form endophytic associations within Theobroma cacao trichomes. Mycology Research 113, 1365-1376. http://dx.doi.org/10.1016/j.mycres.2009.09.004

Barnet HL, Hunter BB. 1998. Illustrated Genera of Imperfect Fungi, 4ed. The American Phytopathological  Society St. Paul Minnesota. APS Press. USA.

CABI. 2016. Improved management strategies for cocoa in Papua New Guinea. Accessed on 17 August 2016 http://www.cabi.org/projects/project/33019

Clay K. 1992. Fungal endophytes of tree leaves. In: Andrews JH, Hirano SS (eds). Microbial Ecologyof Leaves, Berlin: Springer Verlag, 341 – 349.

Dai C, Yu B, Li X. 2008. Screening of endophytic fungi that promote the growth of Euphorbia pekinensis. African Journal of Biotechnology 7(19), 3505–3510.

Dinas Perkebunan dan Hortikultura.  2013.  Statistik Perkebunan. Dinas Perkebunan dan Hortikultura Provinsi Sulawesi Tenggara, Kendari.

Dinas Perkebunan dan Hortikultura. 2015. Statistik Perkebunan. Dinas Perkebunan dan Hortikultura Provinsi Sulawesi Tenggara, Kendari

Dirjen Perkebunan. 2014. Statistik Perkebunan Indonesia Komoditas Kakao 2013-2015. Direktorat Jenderal Perkebunan. 61 hal.

Duoanla-Meli C, Langer E, Moufalo FT. 2013. Fungal endophyte diversity and community patterns in healthy and yellowing leaves of Citrus limon. Fungal Ecology 6, 212–222. http://dx.doi.org/10.1016/j.funeco.2013.01.004.

Gao FK, Dai CC, Liu XZ. 2010. Mechanisms of fungal endophytes in plant protection against pathogens. African Journal of Microbiology Research 4(13), 1346-1351. http://www.academicjournals.org/article/article1380280021_Gao et al.pdf

Guest D, Keane PJ. 2007. Vascular Streak Dieback: A new encounter disease of cacao in Papua New Guinea and Southeast Asia caused by the obligate Basidiomycete Oncobasidium theobromae. Phytopathology 97(12), 1654 – 1657. http://dx.doi.org/10.1094/PHYTO-97-12-1654.

Halimah, Sri-Sukamto. 2007. Intensity of VSD disease on a number of cocoa clones, collections of Center for Coffea and Cocoa Research, Indonesia. Pelita Perkebunan 23, 118–128.

Hanada RE, Pomella AWV, Costa HS, Bezerra JL, Loguercio LL, Pereira JO. 2010. Endophytic fungal diversity in Theobroma cacao (cocoa) and T. grandiflorum (cupuacu) trees and their potential for growth promotion and biocontrol of black-pod disease. Journal Biology 114, 901-910.  https://dx.doi.org/10.1016/j.funbio.2010.08.006

Ibiremo OS, Akanbi OSO, Oloyede AA, Adewale BD. 2014. Comparative evaluation of NPK fertilizer sources on the growth and yield of cocoa in Ibadan, Nigeria. World Journal of Biological Science 2(3), 062-066.

ICCO. 2013. Quarterly bulletin on Cocoa Statistics, 38(1), Cocoa year 2011/2012. (Diunduh, 28 Januari 2016). http://www.icco.org/statistics/quarterlybulletincocoa-statistics.html.

Kalyanasundaram IJ, Nagamuthu, Muthukumaraswamy S. 2015. Antimicrobial activity of endophytic fungis isolated and identified from salt marsh plant in Vellar Estuary. Journal of Microbiology and Antimicrobials 7(2), 13-20. http://dx.doi.org/10.5897/JMA2014.0334

Lu H, Zou WX, Meng JC, Hu J, Tan RX. 2000. New bioactive metabolites produced by Colletotrichum sp., an endophytic fungus in Artemisia annua. Plant Science 151, 67-73. http://dx.doi.org/10.1016/S0168-9452(99)00199-5

McMahon P, Purwantara A, Susilo AW, SriSukamto, Wahab A, Purung H, Hidayat M, Darna I, Taproni Smilja L, Guest D, Keane PJ. 2010. On-farm selection for quality and resistance to pest/diseases of cocoa in Sulawesi: (ii) quality and performance of selections against Phytophthora pod rot and Vascular-streak dieback. International Journal of Pest Management 56(4), 351-361. http://dx.doi.org/10.1080/09670874.2010.503284

Purung H. 2008. The distribution of VSD in Sulawesi. Mars Cocoa Clinic. Palopo.

Rosmana A, Sylvia S, Syamsul B. 2010. Spraying applications on soil and leaves of fungicide Flutriafol to control VSD disease on cocoa plants. Seminar Proceedings and Annual Meeting XX of PEI and PFI, South Sulawesi.

Rosmana A, Papalangi IP, Kannapadan S, Rahim MD, Asman Nasaruddin. 2014. Cultural and pathologenic characterization of Fusarium fungi isolated from dieback branches of cacao. International Journal of Current Research and Academic Review 2(10), 1-6. http://www.ijcrar.com/vol-210/AdeRosmana,etal.pdf

Rubini MR, Ribeiro RTS, Pomella AWV, Maki CS, Araujo WL, dos Santos DR, Azevedo JL. 2005. Diversity of endophyte fungal community of cacao (Theobromae cacao L.) and biological control of Crinipelis pernicosa, causal agent of Witches Broom disease. International Journal of Biological Sciences 1, 24-33. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1140355/

Samuel SGJ, Ismaiel A, Rosmana A, Junaid M, Guest D, McMahon P, Keane P, Purwantara A, Lambert FS, Rodriguez-Carresg M, Cubetag MA. 2012. Vascular Streak Dieback of cacao in Southeast Asia and Melanesia: in planta detection of the patogen and a new taxonomy. Journal of Fungal Biology 116(1), 11-23. http://dx.doi.org/10.1016/j.funbio.2011.07.009

Shekhawat KK, Rai DV, Batra A. 2010. Morphological study of endophytic fungi inhabiting leaves of Melia azedarach L. International Journal of Pharmaceutical Sciences Review and Research 5(3), 177-180.

Simarmata, Rumilla. 2007. Isolation of endophytic microbes from medicinal plant Gynura Procumbens and its potential analysis as antimicrobial agent. Journal Penelitian Hayati 13, 85-90.

Srivastava LM. 2002. Plant Growth and Development, Hormones and Environment. Academic Press, Orlando.

Vega FE, Posada F, Aime MC, Pava-Ripoll M, Infante F, Rehner SA. 2008.  Entomopathogenic fungal endophytes. Biological Control 46, 72–82. http://dx.doi.org/10.1016/j.biocontrol.2008.01.008

Wahab A, Wijayanto T, Taufik M, Bande LS, Gusnawaty, Asaad M, Rahim MD,  Firmansyah AP. 2016. Role of biological agents and cocoa clones to control vascular streak dieback disease (Ceratobasidium theobromae Tallbot and Keane) of cocoa plants. International Journal of Biosciences 9(3), 1-11. http://dx.doi.org/10.12692/ijb/9.3.1-11