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Research Paper | April 1, 2021

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Isolation, identification and characterization of Rhizobium from lentil root nodule

Most. Farhana Begom, Md. Giush Uddin Ahmed, Rebeka Sultana, Md. Zahurul Islam

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Int. J. Biosci.18(4), 22-28, April 2021

DOI: http://dx.doi.org/10.12692/ijb/18.4.22-28

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Abstract

The use of some microorganisms are capable of fixing atmospheric nitrogen can reduce chemical (nitrogen) contamination in the soil. The main purpose of this research paper is to isolate Rhizobium as a nitrogen fixing bacteria from root nodules of lentil (Lens culinaris) and its identification, characterization and hence the production of biofertilizer. In culture initiation ssurvival rates were higher using 2-3% chlorox with 70% ethanol as a disinfection medium and the contamination rate was also lower. The primary inoculation of Rhizobium was effected by cutting and smashed nodules at 30-40 days after sowing of lentil plant. Gram staining, biological microscope and scanned electron microscope observations showed that the bacteria were gram negative, rod shaped, the length to width ratio is 3:1 ‍and even smooth edges. Rhizobium was also positive in all the biochemical tests. In vitro culture initiation, microscopic reviews and biochemical testing for specific microbial isolates are essential for microbial fertilizer production.

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Isolation, identification and characterization of Rhizobium from lentil root nodule

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