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Isolation, identification, characterization and antibiotic susceptibility of Vibrio cholera during 1998-99.

Research Paper | September 1, 2017

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Rahim Shah, Ghazala Parveen, Maria Shoukat, Sofia Khalid, Abdul Hameed

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Int. J. Biosci.11( 3), 135-147, September 2017

DOI: http://dx.doi.org/10.12692/ijb/11.3.135-147


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Vibrio Cholera, causative agent of acute gastrointestinal disease or cholera is a natural inhabitant of aquatic environment. Cholera is endemic disease in Latin America, Southern Asia and parts of Africa, where poor sanitation and seasonal outbreaks are particularly associated with seasonal outbreaks. Large number of outbreaks of Vibrio cholera gastroenteritis in Asian countries indicates the need to evaluate the prevalence of that pathogenic species in different regions of Asia. This study was conducted to ascertain the prevalence and antibiotic resistance of Vibrio cholera in the endemic areas of Pakistan. Samples were collected from epidemic cell of National Institute of Health (NIH) during the time period of July 1998 to 1999, on the basis of reported cases of gastroenteritis/ cholera infections. A total of 172 isolates were collected from the 303 stools and vomitus samples of infected patients and their sensitivity to 18 antimicrobial agents were determined by disk diffusion method.  All the isolates of Vibrio cholera showed 100% resistance to streptomycin and trimethoprim/ sulfamethoxazole throughout the study period. The O139 strain isolated from water was resistant to streptomycin and Kanamycin. In contrast Norfloxicin were found to be very effective with only 4% resistance rate during 1998 while Tobramycin showed the best results with only 1% resistance as compared to resistance percentage of Tetracycline 10%, Erythromycin 16%, Chloramphenicol 17%, Cefamendol 40%, Ampicillin 58%, Nalidixic acid 66%, Nitrofurantion 95% during 1999. The comparison of antibiotic sensitivity showed almost similar pattern of antibiotics sensitivity with little variations due to geographical barriers. Furthermore, the trends of increased resistance to antibiotics indicate that indiscriminate use of antimicrobial agents during hospitalization and self-medication contributed to the emergence of drug resistance in the prevalent strain of Vibrio cholera.


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Isolation, identification, characterization and antibiotic susceptibility of Vibrio cholera during 1998-99.

Adagbada AO, Adesida SA, Nwaokorie FO, Niemogha MT, Coker AO. 2012. Cholera epidemiology in Nigeria: an overview. Pan African Medical Journal, 12(1), Available at : www.ncbi.nlm.nih.gov/pmc/articles/PMC3428179/

Albert MJ. 1996. Epidemiology & molecular biology of Vibrio cholerae O139 Bengal. The Indian journal of medical research, (104), 14-27. Available at : www.ncbi.nlm.nih.gov/pubmed/8783504

Ansaruzzaman M. 1996. Differentiation of Vibrio cholerae O1 isolates with biochemical fingerprinting and comparison with ribotyping. Journal of diarrhoeal diseases research: 248-254. www.jstor.org/stable/23498428.

Ansaruzzaman M. 2004. Cholera in Mozambique, variant of Vibrio cholerae. Emerging infectious diseases, 10(11), 2057. http://dx.doi.org/10.3201/eid1011.040682.

Azman AS. 2012. Urban cholera transmission hotspots and their implications for reactive vaccination: evidence from Bissau city, Guinea bissau. PLoS Negl Trop Dis, 6(11), e1901. https://doi.org/10.1371/journal.pntd.0001901.

Baracchini T. 2016. Seasonality in cholera dynamics: A rainfall-driven model explains the wide range of patterns in endemic areas. Advances in Water Resources. https://doi.org/10.1016/j.advwatres.2016.11.012.

Barua D. 1992. History of cholera, Cholera. Springer, pp. 1-36. Available at: https://link.springer.com/content/pdf/10.1007/978-1-4757-9688-9_1.pdf.

Bauer A, Kirby W, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology 45(4), 493. Available at: www.ncbi.nlm.nih.gov/pubmed/5325707.

Berger S. 2017. Infectious Diseases of Pakistan: 2017 edition. GIDEON Informatics Inc.Available at : www.gideononline.com/ebooks/country/infectious-diseases-of-pakistan/

Centers for Disease Control, Prevention, U.S. Department of Health & Human Services, 2010. Available at: www.cdc.gov/cholera/index.html

Cheesbrough M. 1981. Medical laboratory manual for tropical countries, 1. M. Cheesbrough, 14 Bevills Close, Doddington, Cambridgeshire, PE15 OTT. ISBN10 0750615206,ISBN13 9780750615204

Chitnis D, Sharma K, Kamat R. 1982. Role of somatic antigen of Vibrio cholerae in adhesion to intestinal mucosa. Journal of medical microbiology, 15(1), 53-61. https://doi.org/10.1099/00222615-15-1-53

Coppo A. 1995. Vibrio cholerae in the horn of Africa: epidemiology, plasmids, tetracycline resistance gene amplification, and comparison between O1 and non-O1 strains. The American journal of tropical medicine and hygiene 53(4), 351-359.

Crowe S. 2016. Vibriosis, not cholera: toxigenic Vibrio cholerae non-O1, non-O139 infections in the United States, 1984–2014. Epidemiology and Infection: 1-7. https://doi.org/10.1017/S0950268816001783

Dalhat MM. 2014. Descriptive characterization of the 2010 cholera outbreak in Nigeria. BMC public health, 14(1), 1167. https://doi.org/10.1186/1471-2458-14-1167

Deb B, De, S, Pal S. 1979. Study of an extensive outbreak of cholera in Cooch Behar during 1974. Indian Journal of Medical Research, 70, 691-696. Available at: www.ncbi.nlm.nih.gov/pubmed/535968

Dobre P, MATEI F, Nicolae F. 2014. Main factors affecting biogas production-an overview. Romanian Biotechnological Letters, Romenia, 19(3), 9283-9296. Available at: www.cabdirect.org/cabdirect/abstract/20143271460

Faruque SM. 1997. Molecular analysis of toxigenic Vibrio cholerae O139 Bengal strains isolated in Bangladesh between 1993 and 1996: evidence for emergence of a new clone of the Bengal vibrios. Journal of clinical microbiology 35(9), 2299-2306. Available at : www.ncbi.nlm.nih.gov/pubmed/9276406

Faruque SM. 2004. Genetic diversity and virulence potential of environmental Vibrio cholerae population in a cholera-endemic area. Proceedings of the National Academy of Sciences, 101(7), 2123-2128. www.pnas.org/cgi/doi/10.1073/pnas.0308485100.

Fisher-Hoch S, Khan A, Khan MA, Mintz E, 1993. Vibrio cholerae 0139 in Karachi, Pakistan. The Lancet, 342(8884), 1422-1423. http://dx.doi.org/10.1016/0140-6736(93)92780-W

Fuchs TM. 1998. Molecular mechanisms of bacterial pathogenicity. Naturwissenschaften, 85(3), 99-108. https://doi.org/10.1007/s001140050463

Gillepsie M, Skurray R. 1987. Resistance to antibiotics mediated by target alterations. Antimicrob. Agents Chemother 31, 1648.

Guo Y. 2017. Effects of hydraulic retention time (HRT) on denitrification using waste activated sludge thermal hydrolysis liquid and acidogenic liquid as carbon sources. Bioresource technology, 224, 147-156. https://doi.org/10.1016/j.biortech.2016.11.056

Hatti-Kaul R, Mattiasson B. 2016. Anaerobes in Industrial-and Environmental Biotechnology. Springer, Cham. https://doi.org/10.1007/10_2016_10

Islam MS, Drasar BS, Sack RB. 1993. The aquatic environment as a reservoir of Vibrio cholerae: a review. Journal of diarrhoeal diseases research: 197-206. http://dx.doi.org/10.1155/2013/746254

Khan M, Ara F, Yousuf MO, Ghafoor A. 1989. Outbreak of gastroenteritis in different areas of Pakistan. JPMA. The Journal of the Pakistan Medical Association, 39(6), 151-154. Available at: www.ncbi.nlm.nih.gov/pubmed/2504955

Khan MA. 1988. Gastroenteritis due to vibrio cholerae Eltor Ogawa. JPMA, 38(170).

Lozano R. 2013. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010. The Lancet, 380(9859), 2095-2128. https://doi.org/10.1016/S0140-6736(12)61728-0

Mahalanabis D, Faruque A, Albert M, Salam M, Hoque S. 1994. An epidemic of cholera due to Vibrio cholerae O139 in Dhaka, Bangladesh: clinical and epidemiological features. Epidemiology and Infection, 112(03), 463-471.

Mandal J, Dinoop K, Parija SC. 2012. Increasing antimicrobial resistance of Vibrio cholerae O1 biotype El Tor strains isolated in a tertiary-care centre in India. Journal of Health, Population and Nutrition: 12-16. Available at: www.jstor.org/stable/23500099

Mhalu F, Mmari P, Ijumba J. 1979. Rapid emergence of El Tor vibrio cholera resistant to antimicrobial agents during first six months of fourth cholera epidemic in Tanzania. The Lancet, 313(8112), 345-347.  http://dx.doi.org/10.1016/S0140-6736(79)92889-7

Mintz ED, Tauxe RV. 2013. Cholera in Africa: a closer look and a time for action. Journal of Infectious Diseases, 208(suppl 1), S4-S7. https://doi.org/10.1093/infdis/jit205

Mukhopadhyay A. 1995. Distribution and virulence of Vibrio cholerae belonging to serogroups other than O1 and O139: a nationwide survey. Epidemiology and Infection, 114(01), 65-70. Available at: www.ncbi.nlm.nih.gov/pmc/articles/PMC2271350/

Nitsure S, Dravid M, Jaffari L, Anvikar A. 1997. Gastroenteritis due to Vibrio cholerae El-Tor Ogawa in Dhule. Indian journal of medical sciences, 51(11), 417-419.

Noguerola I, Blanch A. 2008. Identification of Vibrio spp. with a set of dichotomous keys. Journal of Applied Microbiology, 105(1), 175-185. http://dx.doi.org/10.1111/j.13652672.2008.03730.x

Okeke IN. 2005. Antimicrobial resistance in developing countries. Part I: recent trends and current status. The Lancet infectious diseases, 5(8), 481-493. http://dx.doi.org/10.1016/S1473-3099(05)70189-4

Ramamurthy T. 1993. Emergence of novel strain of Vibrio cholerae with epidemic potential in southern and eastern India. The Lancet, 341(8846), 703-704. http://dx.doi.org/10.1016/0140-6736(93)90480-5

Reidl J, Klose KE. 2002. Vibrio cholerae and cholera: out of the water and into the host. FEMS microbiology reviews, 26(2), 125-139. https://doi.org/10.1111/j.1574-6976.2002.tb00605.x

Sack DA, Bradley R, Sack MDS. 2006. Getting serious about cholera. The New England journal of medicine 355(7), 649. https://doi.org/10.1056/NEJMp068144

Sheikh A, Khan A, Malik T, Fisher-Hoch S. 1997. Cholera in a developing megacity; Karachi, Pakistan. Epidemiology and Infection, 119(03): 287-292.Available at: www.ncbi.nlm.nih.gov/pmc/articles/PMC2808999/

Sperandio V, Giron JA, Silveira WD, Kaper JB. 1995. The OmpU outer membrane protein, a potential adherence factor of Vibrio cholerae. Infection and immunity, 63(11), 4433-4438.

Srinivasan P, Gopalakrishnamurthy T, Mohan B, Saravanan S. 2011. Occurrence of Sub Acute Fowl Cholera In a Broiler Flock. Tamilnadu J. Veterinary & Animal Sciences, 7, 45-47. Available at : www.tanuvas.tn.nic.in/tnjvas/tnjvas/vol7(1)/45-47.pdf

Sundaram S, Murthy K. 1984. Occurrence of transferable multi-drug resistance in Vibrio cholerae-01 in an endemic area. Indian Journal of Medical Research, 79,722-727. Availableat: http://imsear.hellis.org/handle/123456789/21926

Tabtieng R. 1989. An epidemic of Vibrio cholerae El Tor Inaba resistant to several antibiotics with a conjugative group C plasmid coding for type II dihydrofolate reductase in Thailand. The American journal of tropical medicine and hygiene, 41(6), 680-686. https://doi.org/10.4269/ajtmh.1989.41.680

Ved’mina E, Givental N, Sobolev V, Ogneva N, Voronin I. 1984. Resistance to antibiotics of Vibrio cholerae and its possible prognostic significance. Antibiotiki, 29(4), 260-263. www.ncbi.nlm.nih.gov/labs/articles/6742803

Weber J. 1994. Epidemic cholera in Ecuador: multidrug–resistance and transmission by water and seafood. Epidemiology and Infection, 112(01), 1-11. www.ncbi.nlm.nih.gov/pubmed/8119348

Wikipedia, The Free Encyclopedia, 2017, Health in Pakistan-Diarrhea. https://en.wikipedia.org/w/index.php?title=Health_in_Pakistan&oldid=772644533

Wilcox M, Cook A, Eley A, Spencer R. 1992. Aeromonas spp as a potential cause of diarrhoea in children. Journal of clinical pathology, 45(11), 959-963. www.ncbi.nlm.nih.gov/pmc/articles/PMC495024