Isolation, molecular identification and phylogenetic analysis of Staphylococcus sciuri isolated from the gut of mulberry silkworm cadaver
Paper Details
Isolation, molecular identification and phylogenetic analysis of Staphylococcus sciuri isolated from the gut of mulberry silkworm cadaver
Abstract
Sericulture is a highly viable industry capable of generating substantial, gainful employment for the people. Bacteria Flacherie is the most serious disease of Bombyx mori; identification of bacterial isolates was done using cultural, morphological and biochemical characteristics. But in the present study, identification of the bacterial isolates from the silkworm cadavers was carried out with modern techniques like 16s RNA gene sequence analysis. On the basis of multiple sequence alignment and the phylogenetic tree constructed by Chromoslite (version 1.2) showed that the bacterial strain isolated as clustered from mulberry silkworm cadavers was the closest homology with Staphylococcus sciuri with 100% similarity and length of 1550 bp.
Bebitha B, Mohan raj P, Mani megalai S, Mohalingam CA. 2016. Silkworm diseasedaignosis through molecular approach and their management, International journal of plant protection 9(1), p 343-357.
Darby AC, Chandler SM, Welburn SC, Douglas 2005. Aphid-symbiotic bacteria cultured in insect cell lines.Applied Environental Microbiology. 71(8), 4833-4839.
Hott JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. 1994. Bergey’s Manual of determinative bacteriology, ninth edition, Williams and wilkins, Baltimore group 17, gram negative cocci, 527-558.
Guo-Ping K, Xi-jie G. 2011. Over view of silkworm pathology in China. African journal of Biotechnology. 10(79), p18046-18056.
Kloos WE, Schleifer KH, Smith RF. 1976. Characterization of Staphylococcus scuiri species nov and its subspecies. International journal of systematic bacteriology 26, p22-37.
Kloos WE, Ballard DN, Webster JA, Hubner RJ, Tomasz A, Couto I, Sloan GL, Dehart HP, Fiedler F, Schubert K, de Lencastre H, Sanches IS, Heath HE, Leblanc PA, Ljungh A. 1997. Ribo type delineation and description of 497 Staphylococcus sciuri subspecies and their potential as reservoirs of 498 methicillin resistance and staphylolytic enzyme genes. International Journal of Systematic Bacteriology 499(47), 313-323.
Paul Vos D, Garrity G, Jones D, Kreig NR, Ludwig W, Rainey FA, Schleifer KH, Whitmann WB. 2009. Bergey’s Manual of systematic bacteriology 3, the firmicules, springler, 392-426, second edition, Springler.
Poinar GO, Thomas GM. 1984. Laboratory guide to insect pathogen and parasites. Plentum press, New York, London.
Rahul K, Moamongba Saikia K, Rabha M, Siva
Prasad V. 2009. Identification and characterization of bacteria causing flacherie in mulberry silkworm Bombyx mori, L. Journal of crop and weed 15(3), p 178-181.
Samson MV, Baig M, Sharma SD, BalaVenkatasubbaiah M, Sasidharan TO, Jolly MS. 1990. Survey on the relative incidence of silkworm diseases in Karnataka. Indian Journal of Sericolgy 29, 248-254.
Schefler WC. 1980. Analysis of variance – Part I & II. In:Statistics for the Biological Sciences, Second Edition, Addison – Wesley Publishing Company, Inc., Philippines, p 121-160.
Selvakumar T, Datta RK. 2013. Role of bacteria streptococcus faecalis & staphylococcus aureus, viruses and their combined infection in causation of flacherie under different environmental conditions. Sericologia 5(1), 54-62, 2013.
Janga Sathish, Gudimalla Prashanthi, Kuntamalla Sujatha, 2022. Isolation, molecular identification and phylogenetic analysis of Staphylococcus sciuri isolated from the gut of mulberry silkworm cadaver. Int. J. Biosci., 20(6), 88-94.
Copyright © 2022 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.