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Research Paper | June 1, 2022

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Isolation, molecular identification and phylogenetic analysis of Staphylococcus sciuri isolated from the gut of mulberry silkworm cadaver

Janga Sathish, Gudimalla Prashanthi, Kuntamalla Sujatha

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Int. J. Biosci.20(6), 88-94, June 2022

DOI: http://dx.doi.org/10.12692/ijb/20.6.88-94

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Abstract

Sericulture is a highly viable industry capable of generating substantial, gainful employment for the people. Bacteria Flacherie is the most serious disease of Bombyx mori; identification of bacterial isolates was done using cultural, morphological and biochemical characteristics. But in the present study, identification of the bacterial isolates from the silkworm cadavers was carried out with modern techniques like 16s RNA gene sequence analysis. On the basis of multiple sequence alignment and the phylogenetic tree constructed by Chromoslite (version 1.2) showed that the bacterial strain isolated as clustered from mulberry silkworm cadavers was the closest homology with Staphylococcus sciuri with 100% similarity and length of 1550 bp.

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Isolation, molecular identification and phylogenetic analysis of Staphylococcus sciuri isolated from the gut of mulberry silkworm cadaver

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