Isolation, screening and adaptation of bacteria isolated from sewage waste water and their ability to degrade pyrene through co-metabolism

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Isolation, screening and adaptation of bacteria isolated from sewage waste water and their ability to degrade pyrene through co-metabolism


Eleven bacterial wild strains were isolated from sewage waste water of PCSIR Laboratory Colony Peshawar, through serial dilution and were adapted for degradation of polycyclic aromatic hydrocarbons (PAHs). These isolates were screened for low molecular weight (LMW) and high molecular weight (HMW) PAHs. Isolated strains were screened on solid nutrient agar and then on mineral media. These bacterial isolates can bring about complete mineralization of (HMW) like pyrene. Isolates W1, W2, W8 and W11 were best on pyrene and phenanthrene respectively. PAHs compounds produced yellow coloration on mineral media plates. This yellow coloration during degradation of PAHs is the characteristic property of bacterial conversion of polyaromatic hydrocarbons to 2-hydroxylmuconic semi-aldehyde through meta-cleavage of catechol. Among these isolates, only W2 was able to grow on pyrene individually and efficiently degraded PAHs mixture. Naphthalene and acenaphthene were completely degraded in 24 hours as revealed from HPLC analysis. Catechol (64.05%) and phthalic acids (31.44%) were detected after 72 hours are the degradation products of phenanthrene and anthracene. 1-hydroxy2-naphthoic acid (63.37%) is the oxidation products of phenanthrene through naphthalene degradation pathway. Increased production of benzocaumarin (32.89-93.13%) between incubation periods of 120-168 hours can be the possible explanation that selected PAHs present in the mixture were degraded through naphthalene pathway. Experiment was performed in triplicate and data was analysed by 2-way ANOVA and level of significance < 0.002.


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