Jasmonic acid influence on the activity of hyp-1 gene in St. John’s wort (Hypericum perforatum)

Paper Details

Research Paper 01/07/2017
Views (251) Download (11)

Jasmonic acid influence on the activity of hyp-1 gene in St. John’s wort (Hypericum perforatum)

Elahe Haqqi-Tilaki, Nader Farsad-Akhtar, Nasser Mahna, Rouhollah Motafakkerazad, Hanieh Mohajjel-Shoja
J. Bio. Env. Sci.11( 1), 76-80, July 2017.
Certificate: JBES 2017 [Generate Certificate]


Considering the importance of hyp-1 gene function in hypericin biosynthesis in Hypericum perforatum, we studied the effect of various concentrations of jasmonic acid on the expression of this gene through qRT-PCR method. In this way, calli from leaf samples of St. John’s wort in vitro grown plants were treated with three concentrations of jasmonic acid (JA) and the activity of hyp-1 gene was evaluated via real-time PCR, 24, 48 and 72 hours after treatment. The results showed that in control samples there was no variation in gene expression. The samples treated with 100 μM JA, a time course increase in gene expression was observed. The same trend was seen with 500 μM JA. Accordingly, the maximum hyp-1 gene expression was witnessed in 72 hours after treatment, while the minimum was in 24 hours. In all evaluated times, increasing the concentration of JA resulted in higher expression of hyp-1 gene. Moreover, the expression of hyp-1 24 hours after increased up to about 1, 1.5 and 3 fold in the 0, 100 and 500 μM JA treatments, respectively. A similar trend was observed after 48 h, and the expression of the gene went higher up to 4 fold in 500 μM JA. After 72 h, a 5.5 fold increase was recorded in the maximum concentration of JA. It can be concluded that with increasing the concentration of JA as well as in time course, a significant increment could be seen in the expression of hyp-1 gene.


Bais HP, Walker TS, Grew JJ, Vivanco JM. 2002. Factors affecting the growth of cell suspension cultures of Hypericum perforatum L. (St. John’s Wort) and production of hypericin. In vitro cellular & Developmental Biology-Plant 38, 58-65.

Deepak S, Kottapalli KR, Rakwal R, Oros G, Rangappa KS, Iwahashi H, Masuo Y, Agrawal GK. 2007. Real time PCR: revolutionizing detection and expression analysis of genes. Current genomics 8, 234- 251.

Dias ACP, Tomas-Barber FA, Fernandes-Ferreira M, Ferreres F. 1998. Unusual flavonoids produced by callus of Hypericum perforatum. Phytochemistry 48, 1165-1168.

Ferrari F, Pasqua G, Monacelli B, Botta B. 2002. Xanthones from calli of Hypericum perforatum subsp. perforatum. Natural product research 19, 171-176.

Filandrinos D, Yentsch TR, Meyers KL. 2006. Herbal Products: Toxicology and Clinical Pharmacology. New York: Humana Press p. 71-95.

Gadzovska S, Maury S, Delaunay A, Spasenoski M, Joseph C, Hagege D. 2007. Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones. Plant Cell, Tissue and Organ Culture 89, 1-13.

Gadzovska S, Maury S, Ounnar S, Righezza M, Kascakova S, Refregiers M, Spasenoski M, Joseph C, Hagege D. 2005. Identification and quantification of hypericin and pseudohypericin in different Hypericum perforatum L. in vitro cultures. Plant Physiology and Biochemistry 43, 591-601.

Gadzovska S, Tusevski O, Antevski S, Atanasova-Pancevska N, Petreska J, Stefova M, Kungulovski D, Spasenoski M. 2012. Secondary metabolite production in Hypericum perforatum L. cell suspensions upon elicitation with fungal mycelia from Aspergillus flavus. Archives of Biological Science Belgrade 64, 113-121.

Gibson D, Sirvent T. 2002. Induction of hypericins and hyperforin in Hypericum perforatum L. in response to biotic and chemical elicitors. Physiological and Molecular Plant Pathology 60, 311-320.

Kartning T, Gobel I. 1996. Production of hypericin, pseudohypericin and flavonoids in cell cultures of various Hypericum species and their chemotypes. Planta Medica 62, 51-53.

Pasqua G, Avato P, Monacelli B, Santamaria AR, Argentieri MP. 2003. Metabolites in cell suspension cultures, calli, and invitro regenerated organs of Hypericum perforatum cv. Topas. Plant Science 165, 977-982.

Santarem ER, Astarita LV. 2003. Multiple shoot formation in Hypericum perforatum L. and hypericin production. Brazil Journal of Plant Physiology 15, 43-47.

Vardapetyan HR, Oganesyan AA, Kabasakalyan EE, Tiratsuyan SG. 2006. The influence of some elicitors on growth and morphogenesis of Hypericum perforatum L. callus cultures. Russian Journal of Developmental Biology 37, 350-353.

Xu MJ, Dong JF, Zhu MY. 2005. Nitric oxide mediates the fungal elicitor induced Hypericin production of Hypericum perforatum cell suspension cultures through a Jasmonic-acid-dependent signal pathway. Journal of Plant Physiology 139, 991-998.

Zhao J, Davis LC, Verpoorte R. 2005. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances 23, 283-333.