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Extraction, characterization, and workability of natural biological dye from local berries: A laboratory protocol for rural schools

By: Shella B Cacatian

Key Words: Characterization, Local berries, Natural biological dye, Protocol, Workability

J. Bio. Env. Sci. 18(5), 8-18, May 2021.

Certification: jbes 2021 0327 [Generate Certificate]


The application of natural biological dyes finds its use as an alternative to synthetic stains in temporary and permanent mounts. Extract of natural stains from peels of Lubeg (Syzygium lineatum), Bignay (Antidesma bunius), and Duhat (Syzygium cumini) fruits were made to test their staining potential on plant and animal tissues. The study utilized the Completely Randomized Design to compare the staining property of the peels. It used the Analysis of Variance (ANOVA) and Least Significant Differences (LSD) to ascertain the differences between the treatments. The natural biological dyes, in comparison with the synthetic dye, stained the cross-section of the Wiregrass and Narra stems, longitudinal section of an Autumn crocus, and hepatic tissues of a frog with remarkably deeper color. They are more effective in highlighting structures of a cross-section of a wiregrass stem and longitudinal section of an Autumn crocus, and they have a higher bulk density, which is an essential requirement of container volume. However, the synthetic dye shows higher absorbing capacity and solubility than the natural biological dyes. The success of producing a low-cost biological stain is achieved using Lubeg, Bignay, and Duhat fruits. The natural biological dyes used in this study could serve as a useful alternative to synthetic dyes.

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Extraction, characterization, and workability of natural biological dye from local berries: A laboratory protocol for rural schools

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Shella B Cacatian.
Extraction, characterization, and workability of natural biological dye from local berries: A laboratory protocol for rural schools.
J. Bio. Env. Sci. 18(5), 8-18, May 2021.
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