Genotoxicity and oxidative stress analysis in the Catla catla treated with ZnO NPs
Paper Details
Genotoxicity and oxidative stress analysis in the Catla catla treated with ZnO NPs
Abstract
In the history of human beings nanotechnology is one of the fastest growing industries and has been referred to as next industrial revolution. Zinc oxide nanoparticle (ZnO NPs) high concentration causes genotoxicity in the aquatic animals which is mainly due to the excess production of the oxidative stress. The treatment of ZnO NPs produces micronuclei comet and oxidative stress in the dose dependant manners. In the present study, the evaluation of genotoxicity was observed by micronuclei test and comet assay. The result showed that the increase concentration of ZnO NPs increase of the frequency of micronuclei and comets significantly. High frequency of comets (22.51±0.62) and micronuclei (0.557±0.081) was recorded at 80 mgL-1 after 28 days of treatment. This might be due to depletion of genetic repair mechanism. The oxidative stress was evaluated by observing the levels of SOD (Superoxide dismutase), CAT (Catalase), GST (Glutathione S-transferase), MDA (Malonetialdohyde content) and GSH (Reduce glutathione) after 7and 28 days of the ZnO NPs treatment. The gills and liver tissues exhibited a decrease in the activity of CAT and GST at every treatment in a dose dependent manner. However, the activity of SOD increased in response to ZnO NPs as MDA and GSH. This study concluded, ZnO NPs are genotoxic in the aquatic organisms and produce the oxidative stress at the elevated level.
Adedeji O, Okerentugba P, Okonko I. 2012. Use of molecular, biochemical and cellular biomarkers in monitoring environmental and aquatic pollution. Nature and Science 10, 83-104.
Aebi H. 1984. Catalase in vitro. Methods in enzymology 105, 121-126.
Ali D, Nagpure N, Kumar S, Kumar R, Kushwaha B. 2008. Genotoxicity assessment of acute exposure of chlorpyrifos to freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis. Chemosphere 71(10), 1823-1831.
Andem A, Agbor R, Ekpo I. 2013. Review on comet assay: a reliable tool for assessing dna damage in animal models. Journal of Current Research in Science 1(6), 405.
Asghar MS, Qureshi NA, Jabeen F, Khan MS, Shakeel M, Noureen A. 2015. Toxicity of zinc nanoparticles in fish: a critical review. Journal of Biodiversity and Environmental Sciences 7(1), 431-439.
Begum A, Harikrishna S, Khan I. 2009. Analysis of heavy metals in water, sediments and fish samples of Madivala lakes of Bangalore, Karnataka. International Journal of ChemTech Research 1(2), 245-249.
Brendler-Schwaab S, Hartmann A, Pfuhler S, Speit G. 2005. The in vivo comet assay: use and status in genotoxicity testing. Mutagenesis 20(4), 245-254.
Buege JA, Aust SD. 1978. [30] Microsomal lipid peroxidation. Methods in enzymology 52, 302-310.
Buerki-Thurnherr T, Xiao L, Diener L, Arslan O, Hirsch C, Maeder-Althaus X, Grieder K, Wampfler B, Mathur S, Wick P. 2013. In vitro mechanistic study towards a better understanding of ZnO nanoparticle toxicity. Nanotoxicology 7(4), 402-416.
Buschini A, Martino A, Gustavino B, Monfrinotti M, Poli P, Rossi C, Santoro M, Dörr A, Rizzoni M. 2004. Comet assay and micronucleus test in circulating erythrocytes of Cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 557(2), 119-129.
Cavalcanti B, Ferreira J, Moura D, Rosa R, Furtado G, Burbano R, Silveira E, Lima M, Camara C, Saffi J. 2010. Structure–mutagenicity relationship of kaurenoic acid from Xylopia sericeae (Annonaceae). Mutation Research/Genetic Toxicology and Environmental Mutagenesis 701(2), 153-163.
Çavaş T, Ergene-Gözükara S. 2005. Induction of micronuclei and nuclear abnormalities in Oreochromis niloticus following exposure to petroleum refinery and chromium processing plant effluents. Aquatic Toxicology 74(3), 264-271.
Habig WH, Pabst MJ, Jakoby WB. 1974. Glutathione S-transferases the first enzymatic step in mercapturic acid formation. Journal of biological Chemistry 249(22), 7130-7139.
Jollow D, Mitchell J, Zampaglione Na, Gillette J. 1974. Bromobenzene-induced liver necrosis. Protective role of glutathione and evidence for 3, 4-bromobenzene oxide as the hepatotoxic metabolite. Pharmacology 11(3), 151-169.
Kirsch-Volders M, Sofuni T, Aardema M, Albertini S, Eastmond D, Fenech M, Ishidate M, Kirchner S, Lorge E, Morita T. 2003. Report from the in vitro micronucleus assay working group. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 540(2), 153-163.
Kumaravel T, Jha AN. 2006. Reliable Comet assay measurements for detecting DNA damage induced by ionising radiation and chemicals. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 605(1), 7-16.
Kumari M, Khan SS, Pakrashi S, Mukherjee A, Chandrasekaran N. 2011. Cytogenetic and genotoxic effects of zinc oxide nanoparticles on root cells of Allium cepa. Journal of hazardous materials 190(1), 613-621.
Livingstone MBE, Black AE. 2003. Markers of the validity of reported energy intake. The Journal of nutrition 133(3), 895S-920S.
Maluf SW. 2004. Monitoring DNA damage following radiation exposure using cytokinesis–block micronucleus method and alkaline single-cell gel electrophoresis. Clinica Chimica Acta 347(1), 15-24.
Martin P, Leibovich SJ. 2005. Inflammatory cells during wound repair: the good, the bad and the ugly. Trends in cell biology 15(11), 599-607.
Memısogullari R, Taysi S, Bakan E, Capoglu I. 2003. Antioxidant status and lipid peroxidation in type II diabetes mellitus. Cell Biochem. Funct 21, 291-296.
Nel A, Xia T, Mädler L, Li N. 2006. Toxic potential of materials at the nanolevel. Science 311(5761), 622-627.
Olive PL, Banáth JP. 2006. The comet assay: a method to measure DNA damage in individual cells. NATURE PROTOCOLS-ELECTRONIC EDITION-1(1), 23.
Pati R, Das I, Mehta RK, Sahu R, Sonawane A. 2016. Zinc-oxide nanoparticles exhibit genotoxic, clastogenic, cytotoxic and actin depolymerization effects by inducing oxidative stress responses in macrophages and adult mice. Toxicological Sciences, kfw010.
Payá M, Halliwell B, Hoult JRS. 1992. Interactions of a series of coumarins with reactive oxygen species: Scavenging of superoxide, hypochlorous acid and hydroxyl radicals. Biochemical Pharmacology 44(2), 205-214. http://dx.doi.org/10.1016/0006-2952(92)90002-Z
Peixoto AL, Pereira-Moura MVL. 2008. A new genus of Monimiaceae from the Atlantic Coastal Forest in south-eastern Brazil. Kew Bulletin 63(1), 137-141.
Sancar A, Lindsey-Boltz LA, Ünsal-Kaçmaz K, Linn S. 2004. Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints. Annual review of biochemistry 73(1), 39-85.
Singh NP, McCoy MT,Tice RR, Schneider EL. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research 175(1), 184-191. http://dx.doi.org/10.1016/0014-4827(88)90265-0
Tang W,Shan B, Zhang H, Zhang W, Zhao Y, Ding Y, Rong N, Zhu X. 2014. Heavy metal contamination in the surface sediments of representative limnetic ecosystems in eastern China. Scientific reports 4.
Tice R, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu J, Sasaki Y. 2000. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environmental and molecular mutagenesis 35(3), 206-221.
Van der Oost R, Beyer J, Vermeulen NP. 2003. Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environmental toxicology and pharmacology 13(2), 57-149.
Van Goethem F, Lison D, Kirsch-Volders M. 1997. Comparative evaluation of the in vitro micronucleus test and the alkaline single cell gel electrophoresis assay for the detection of DNA damaging agents: genotoxic effects of cobalt powder, tungsten carbide and cobalt–tungsten carbide. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 392(1), 31-43.
Wojewoda M, Duszyński J, Szczepanowska J. 2010. Antioxidant defence systems and generation of reactive oxygen species in osteosarcoma cells with defective mitochondria: effect of selenium. Biochimica et Biophysica Acta (BBA)-Bioenergetics 1797(6), 890-896.
Zhou L. 2015. Toxicity evaluation and medical application of multi-walled carbon nanotubes.
Zhu X, Wang J, Zhang X, Chang Y, Chen Y. 2009b. The impact of ZnO nanoparticle aggregates on the embryonic development of zebrafish (Danio rerio). Nanotechnology 20(19), 195103.
Muhammad Saleem Asghar, Naureen Aziz Quershi, Farhat Jabeen, Muhammad Shakeel, Muhammad Saleem Khan (2016), Genotoxicity and oxidative stress analysis in the Catla catla treated with ZnO NPs; JBES, V8, N4, April, P91-101
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