Karyotype analysis and meiosis in Coryphosima stenoptera producta (Walker) and Chirista compta (Walker) (Orthoptera: Acrididae: Acridinae) from Cameroon

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Karyotype analysis and meiosis in Coryphosima stenoptera producta (Walker) and Chirista compta (Walker) (Orthoptera: Acrididae: Acridinae) from Cameroon

Richard Akwanjoh Seino, Sévilor Kekeunou, Tonleu Ingrid Dongmo, Yacouba Manjeli
Int. J. Biosci.2( 12), 168-176, December 2012.
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Abstract

This article describes the hitherto unknown karyotypes of the short-horned grasshoppers Coryphosima stenoptera producta (Walker) and Chirista compta (Walker) that belong to the family Acrididae and subfamily Acridinae of the order Orthoptera. Chromosomes smears prepared by the lactic-propionic-orcein squash technique and chromosome analysis performed in the two species revealed a conserved karyotype of 2N= 23, XO in males composed exclusively of acrocentric chromosomes. The chromosomes occurred in size groups of long (L), medium (M) and short (S). In C. stenoptera producta were 2LL + 6MM + 3SS chromosomes whereas in C. compta were 4LL + 4MM + 3SS chromosomes. The X chromosome was medium in size in both species with mean lengths of 5.60μm ± 0.56 in C. s. producta and 7.3 μm ± 0.52 in C. compta. The mean chiasma frequency was found to be 12.20 ± 0.77 and 16.20 ± 0.72 in C. stenoptera producta and C. compta respectively. The significantly lower chiasma frequency found in C. stenoptera producta could have been due to the consistent absence of bivalents with 3 or more chiasmata in this population. Bivalents with 1 chiasma contributed most to cell chiasma frequency in C. stenoptera producta, while bivalents with 2 chiasmata contributed most to cell chiasma frequency in C. compta. This article aims to offer some basal data for the cytotaxonomy of the Orthoptera.

VIEWS 3

Bridle JR, De la Torre J, Bella JL, Butlin RK, Gosalvez J. 2002. Low levels of chromosomal differentiation between the grasshoppers Chorthippus brunneus and Chrothippus jacobsi (Orthoptera – Acrididae). Genetica, 114, 121 – 127.

Bugrov AG. 1996. Karyotypes of the short-horned Orthopteran insects (Orthoptera, Caelifera). From Russia, Kazakhstan, Central Asia and the Caucasus. Folia Biologica (Krakow) 44, 15-25.

Bugrov AG, Warchalowska-Sliwa E, Vysotskaya L. 1999.  Karyotype  features of Eyprepocnemidinae grasshoppers from Russia and central Asia with reference to the B-chromosome in Eyprepocnemis plorans (Charp.) Folia Biologica (Krakow) 47(3-4), 97-104.

Bugrov AG, Warchalowska- Sliwa E, Tatsuta H, Akimoto S. 2002. Chromosome polymorphism and C – banding variation of the brachypterus grasshopper Podisma sapporensis Shir. (Orthoptera: Acrididae) in Hokkiado, Northern Japan. Folia Biologica (Krakow), 50 (1 –2), 102

Chadha P, Mehta A. 2011. Chromosome study in few species of Acridids (Acrididae: Tryxalinae): Karyotypic analysis and distribution patterns of constitutive heterochromatin. Journal of Entomology and Nematology, 3 (1), 14 – 19

Clewer AG, Scarisbrick DH. 2001. Practical Statistics and Experimental Design for Plant and Crop Science. John Wiley & Sons.

Cody RP, Jeffrey KS. 2006. Applied Statistics and the SAS Programming Language (5th edn). Prentice Hall: New Jersey.

Dirsh VM.1975. The African genera of AcridAcridoidea. Cambridge.171pp.

Hewitt GM. 1979. Grasshoppers and crickets. Animal cytogenetics. Vol;3: Insecta. I. Orthoptera. Gebruger. Borntraeger ED. Berlin Stuttgartnn.

Mesa A, Ferreira A, Carbonell CSC. 1982. Cariologia de los Acridoideos Neotropicales: Estado actual de su conocimiento y nuevas contribuciones. Ann Soc Ent Fr, 18, 507-526.

Mestre & Chiffaud. 2006. Catalogue et atlas des acridiens d’Afrique de l’Ouest 265 Orthoptera species file (OSF). 2011.

Paris conference. 1971. Standardization in Human cytogenetics. Birth Defects: Original Article Series 8: 7, 1972 New York: The National Foundation (Reprinted in Cytogenetics 11, 1313 – 362 (1972)

Ren Bing-Zhong Na Li, Wen – Juan Wei, Li-ming Wang. 2008. C-band karyotypes of two species of Primnoa (Orthoptera – Catantopidae) from Northeast China. Zootaxa, 1679, 63 – 68.

Rocha MF, Souza MJ, Moura PC. 2004. Karyotype analysis, constitutive heterochromatin and NOR distribution in five grasshopper species of the subfamily Leptysminae (Acrididae). Caryologia, 57, 107 – 116.

Seetharama  M,  Kanale  SS,  Mundkur  JH. 2004. Non banded and C- banded karyotypes of ten species of short horned grasshoppers (Acrididae) from South India. Cytologia, 69 (2), 167 – 174.

Seino RA. 1989. Cytogenetic characterization of seven species of Acridomorphoid grasshoppers. M.Phil. dissertation, University of Lagos, Nigeria, 60 – 95.

Seino RA. Akongnui T. 2010. Meiotic study of Acrida turrita (Linnaeus 1758), Paracinema luculenta Karsch 1896 and Morphacris fasciata (Thunberg 1815) (Orthoptera: Acrididae). International  Journal of Biological and Chemical Sciences. 4 (6), 1914 -1921.

Seino RA, Dongmo IT, Manjeli. Y. 2012. Cytogenetic characterisation of Taphronota thaelephora Stal. 1873 (Orthoptera: Pyrgomorphidae) from Cameroon. II. Description of mitotic chromosomes. International Journal for Biological and Chemical Sciences Vol. 6 (4), 1624 – 1632.

Sharma T, Gautam DC. 2002. Karyotypic studies of eleven species of grasshoppers from North – Western Himalayas. Nucleus, 45(1 – 2), 27 – 35

Souza MJ, De Melo NF. 2007. Chromosome study in Schistocerca (Orthoptera – Acrididae – Cyrtacanthacridinae) : Karyotypes and distribution pattern of constitutive heterochromatin and nucleolus organizer regions (NORs). Genetics and Molecular Biology 30 (1).

Turkoglu, S, Koca, S. 2002. Chromosomes of Oedipoda schochi schochi and Acrotylus insbricus (Orthoptera: Acrdidae: Oedipodinae). Karyotypes and C-band and G-band patterns. Turkey Journal of Zoology, 26, 327-332.

Verma PS, Agarwal VK. 2005. Cell Biology, Molecular Biology, Evolution and Ecology. S Chand & Company Ltd. Ram Nagar, New Delhi 110 055.

White MJD. 1973. Animal cytology and evolution. 3rd edition. The Cambridge University Press. p. 961.

William GO, Ogunbiyi, BI. 1995. Chromosome morphology and meiosis in Zonocerus variegatus L. (Orthoptera, Pyrgomorphidae). Cytologia, 60, 111-116.

Warchalowska-Sliwa E, Kostia D, Sliwa L. 2002. Cytological and morphological differences between two species of the genus Tettigonia (Orthoptera, Tettigonidea) from Korea. Folia Biologica (Krakow), Vol. 50 (1-2), 23-28.