Larval development of black soldier fly (Hermetia illucens Linnaues, 1758) on organophosphate-treated cabbage
Paper Details
Larval development of black soldier fly (Hermetia illucens Linnaues, 1758) on organophosphate-treated cabbage
Abstract
The study conducted in Balili, La Trinidad, Benguet from November 2024 to February 2025 aimed to evaluate the effects of organophosphates (profenofos, malathion, and indoxacarb) on the larval development of Black Soldier Fly (BSF). It assessed growth, development, survival of BSF larvae fed with organophosphate-treated cabbage, organophosphate residue levels, crude protein content of larvae, and the toxicity of organophosphates on BSF larvae. The duration of larvae from 2nd to 6th instar was consistent across treated and untreated groups, ranging from 20 to 23 days with some significant differences in body measurements, excluding 2nd and 3rd instar larvae. Survival rates were significantly different for larvae treated with organophosphates compared to indoxacarb, with malathion-treated larvae showing a survival rate of 30.81%, whereas profenofos and indoxacarb resulted in zero and 0.61% survival rates, respectively, against a control survival rate of 23.61%. Residue levels in cabbage were medium for indoxacarb and low for profenofos and malathion, with no residues found in BSF larvae and frass. Notably, larvae fed malathion treated cabbage had the highest crude protein content at 15.46%, while control larvae had 15.39%. Overall, mortality among BSF larvae varied significantly, with rates from 69.19% to 100%; untreated larvae had a mortality rate of 76.39%, while indoxacarb caused the highest mortality at 100%, followed closely by profenofos at 99.39%.
Aidoo O, Fernando I, Nisa K, Rahayu T, Rasool A, Siddiqui S. 2023. Effects of undesired substances and their bioaccumulation on the black soldier fly larvae, Hermetia illucens (Diptera: Stratiomyidae): A literature review. https://pubmed.ncbi.nlm.nih.gov/37291225/
Alimondo L. 2019. Benguet tops crop production in Cordillera. https://www.sunstar.com.ph/baguio/local-news/benguet-tops-crop-production-in-cordillera
Alsuhaibani A, Brestic M, Choudhury S, Gaber A, Gangulay P, Das A, Hossain A, Mondal T, Patra S, Rashit R, Roy S. 2022. Persistence and exposure assessment of insecticide indoxacarb residues in vegetables. https://pmc.ncbi.nlm.nih.gov/articles/PMC9131938/
Anacin C, Fomeg-as D, Gomez Jr R, Lasangen W, Reyes G. 2017. Detection of organophosphate residues in selected crops in Benguet and Mt. Province, Philippines. 28, 30. https://ovcre.uplb.edu.ph/journalsuplb/index.php/JESAM/article/view/121
Anukun P, Laksanawimol P, Thancharoen A. 2024. Use of different dry materials to control the moisture in a black soldier fly (Hermetia illucens) rearing substrate. https://pmc.ncbi.nlm.nih.gov/articles/PMC10981887/
Arpilia Z, Aristi H, Haridjani N, Mudji E, Soepranianondo K. 2020. Levels of protein and fat produced by black soldier fly (Hermetia illucens) larvae in the bioconversion of organic waste. 1. https://www.researchgate.net/publication/339283520_Levels_of_protein_and_fat_produced_by_black_soldier_fly_hementia_illucens_larvae_in_the_bioconversion_of_organic_waste
Bailey D, Cannella L, Ferrarezi R, Nassef A. 2016. UVI/AES Annual Report 2016: Alternative sources of food for aquaponics in the U.S. Virgin Islands: A case study with black soldier flies. https://www.researchgate.net/publication/311847451_UVIAES_Annual_Report_2016_Alternative_Sources_of_Food_for_Aquaponics_in_the_US_Virgin_Islands_A_Case_Study_with_Black_Soldier_Flies
Bajet C, Cruz E, Galao A, Sarmiento J. 2023. Role of farmers’ training on improving pesticide management and food safety in conventional cabbage and eggplant production. https://www.researchgate.net/publication/374346382_Role_of_Farmers%27_Training_on_Improving_Pesticide_Management_and_Food_Safety_in_Conventional_Cabbage_and_Eggplant_Production
Chatterjee S, Kushwaaha M, Verma S. 2021. Profenofos, an acetylcholinesterase-inhibiting organophosphorus pesticide: A short review of its usage, toxicity, and biodegradation. https://www.researchgate.net/figure/Toxicity-of-profenofos-in-different-living-organisms_fig4_348296891
Cheseto X, Dicke M, Ekesi S, Van Loon JJA, Osuga IM, Tanga CM. 2020. Nutritional compositions of black soldier fly larvae feeding on agro-industrial by-products. https://onlinelibrary.wiley.com/doi/full/10.1111/eea.12940
Lievens S, Poma G, De Smet J, Van Campenhout L, Covaci A, Van Der Borght M. 2021. Chemical safety of black soldier fly larvae (Hermetia illucens), knowledge gaps and recommendations for future research: a critical review. Journal of Insects as Food and Feed 7(4), 383-396. https://doi.org/10.3920/JIFF2020.0081
De Rijk TC, Van Der Fels-Klerx HJ, Van Loon JJA, Meijer N. 2021. Effects of insecticides on mortality, growth and bioaccumulation in black soldier fly (Hermetia illucens) larvae. PLOS ONE 16(4), e0249362. https://doi.org/10.1371/journal.pone.0249362
Demeter Ghana Ltd. 2025. Understanding the post-harvest interval (PHI) in crop production. https://www.demeterghana.com/blog-articles/understanding-the-post-harvest-interval-phi-in-crop-production-f378
Diener S, Lalander C, Vinnerås B, Zurbrügg C. 2019. Effects of feedstock on larval development and process efficiency in waste treatment with black soldier fly (Hermetia illucens). Journal of Cleaner Production 208, 211–219. https://doi.org/10.1016/j.jclepro.2018.10.017
Delima PC. 2012. Detection of Carbamates and Organophosphorus Pesticides and Residues in Selected Vegetables in Northern Philippines. International Journal of Ecology and Conservation 1, 1-12. https://doi.org/10.47125/jesam/2017_2/04
Kumar P, Ramarajan S, Radha M, Murugesan AG. 2015. Effect of inorganic fertilizers on mortality and ovicidal action of dengue vector, Aedes aegypti L. (Diptera: Culicidae). International Journal of Mosquito Research 2(4), 36–42.
Liu Y, Wang Z, Chen H. 2021. Enzymatic mechanisms involved in the biodegradation of organophosphate pesticides by black soldier fly larvae (Hermetia illucens). Environmental Science & Technology 55(4), 2456–2465.
Lu J. 2010. Analysis of trends of the types of pesticide used, residues and related factors among farmers in the largest vegetable-producing area in the Philippines. Journal of Rural Medicine 5(2), 184–189.
Newhart KL. 2006. Environmental fate of malathion. California Environmental Protection Agency, Department of Pesticide Regulation, Environmental Monitoring Branch, Sacramento, CA.
Pacleb J. 2021. Biology of black soldier fly (Hermetia illucens Linn.) on different substrates at La Trinidad, Benguet. Bachelor’s Thesis, Benguet State University.
Spranghers T, Schillewaert S, Wouters F. 2018. Nutritional requirements of BSF larvae [Conference presentation]. VIVES University of Applied Sciences, Belgium. DOI: 10.13140/RG.2.2.27483.00804
Tomberlin JK, Sheppard DC, Joyce JA. 2002. Susceptibility of black soldier fly (Diptera: Stratiomyidae) larvae and adults to four insecticides. Journal of Economic Entomology 95, 598–602.
Septer C. Sucdad*, 2026. Larval development of black soldier fly (Hermetia illucens Linnaues, 1758) on organophosphate-treated cabbage. Int. J. Agron. Agric. Res., 28(6), 9-18.
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