Larvicidal activity of Gmelina arborea fruit extract against dengue vector Aedes aegypti mosquito
Paper Details
Larvicidal activity of Gmelina arborea fruit extract against dengue vector Aedes aegypti mosquito
Abstract
Aedes aegypti, the principal vector of dengue virus has caused alarming health worldwide. There are available commercial mosquito insecticides, but the vectors develop resistance to these and cause harm and pose negative effects on living organisms and the environment. This study was conducted to evaluate the larvicidal activity of Gmelina arborea fruit extract as a biological control agent against 4th instar A. aegypti mosquito larvae. This study applies the control group and variation of treatment using the different concentration of G. arborea fruit extract (25, 50, 75, and 100 mg/L respectively) exposed to 15 batches of 10 4th instars A. aegypti larvae. The data obtained were analyzed using the mean percentage, One Way-ANOVA at 0.05 level of significance and probit analysis. Based on the results, the 100 mg/L concentration of fruit extract obtained the 100% mortality while the control group has no observed mortality of A. aegypti larvae after 24 hours of treatment. Moreover, there is a significant difference (F=33.88, p<0.05) between the control and the experimental group with varying concentration of G. arborea fruit extract. The lethal concentration (LC50 and LC90) values of G. arborea fruit extractwere 56.23 mg/L and 74.13 mg/L which means it is toxic to the mosquito larvae sample. The results showed great potential for the production of organic insecticides from the G. arborea fruit extract to control the A. aegypti, a vector of the dengue virus.
Abdulkarim A, Sadiq Y, Gabriel OA, Abdulkadir UZ, Ezzeldin AM. 2005. Evaluation of five medicinal plants used in diarrhoeal treatment in Nigeria.Journal of Ethnopharmacology 101(1-3), 27-30. https://doi.org/10.1016/j.jep.2005.03.025
Alagesaboopathi C. 2011. Antimicrobial screening of selected medicinal plants in Tamilnadu, India. African Journal of Microbiology Research 5(6), 617-621.
Ambujakshi HR, Shyamnanda TH. 2009. Anthelmintic activity of Gmelina arborea roxb. Leaves extract. Indian Journal of Pharmaceutical Research Development 9(1), 1-5.
Anjum SI, Hussain S, Attaullah M, Khan HU, Khattak B, Fouad H. 2016.Evaluation of the larvicidal potential of Calotropis procera plant extract against Culex pipiens. International Journal of Mosquito Research 3(6), 01-05.
Arnason J, Philogene B, Morand P. 1989. Insecticides of Plant Origin. American Chemical Society Journal 387, 213. https://pubs.acs.org/doi/pdf/10.1021/bk-1989-0387.pr001
Arora C, Tamrakar V. 2017. Gmelina arborea: Chemical constituents, Pharmacological activities and applications. International Journal of Phytomedicine 9, 528-542. http://dx.doi.org/10.5138/09750185.2149
Attanayake AP, Jayatilaka KAPW, Pathirana C, Mudduwa LKB. 2015. Antioxidant activity of Gmelina arborea Roxb. (Verbenaceae) bark extract: In vivo and in vitro study.Journal of Medical Nutrition and Nutraceuticals 4, 32. http://dx.doi.org/10.4103/2278-019X.146159
Bebelli G, Jeffries C, Walker T. 2016. Biological control of mosquito vectors: past, present, and future. Insects 7(4), 52. https://doi.org/10.3390/insects7040052
Chantal NKC. 2009. On the Composition in Secondary Metabolites and the antioxidant activity of crude extracts from Gmelina arborea roxb. (Verbanaceae) from Côte d’Ivoire, West Africa: Analysis by Thin Layer Chromatography. European Journal of Scientific Research 36, 161.
Chellappan DR, Pemiah B. 2014. Pharmacognostical, Phytochemical and In vivo gastroprotective investigation of Gmelina arborea.International Journal of Pharmacy and Pharmaceutical Sciences 6(4), 153-157.
Cheng SS, Chang HT, Chang ST, Tsai KH, Chen WJ. 2003. Bioactivity of Selected Plant Essential Oils Against the Yellow Fever Mosquito Aedes aegypti larvae.Bioresource Technol 89(1), 99–102. https://doi.org/10.1016/S0960-8524(03)00008-7
Das NG, Goswami D, Rabha B. 2007. Preliminary Evaluation of Mosquito Larvicidal Efficacy of Plant Extracts.Journal of vector borne diseases 44(2), 145-148.
Dixit AK, Sudurshan M. 2011. Review of flora of anti-Diabetic plants of Puducherry Ut. Internationl Journal of Applied Biology and Pharmaceutical Technology 2, 455.
El-Mahmood AM, Doughari JH, Kiman HS. 2010. In vitro antimicrobial activity of crude leaf and bark extracts of Gmelina arborea (Roxb) against some pathogenic species of Enterobacteriaceae. African Journal of Pharmacy and Pharmacology 4(6), 355 361. http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.401.8375&rep=rep1&type=pdf
Florence AR, Solomon J. 2016. Larvicidal activity of Gmelina asiatica L. leaf extracts against Aedes aegypti and Culex quinquefasciatus. Annals of Biological Research, 7(1), 12-20
Gangwar AK, Ghosh AK, Hoque M, Saxena V. 2013. Analgesic Activity of Gmelina arborea Roxb in colony bred swiss mice and wister rats.International Journal of Pharmacognosy and Phytochemical Research 5(1), 66-67.
Ghosh A, Chowdhruy N, Chandra G. 2012. Plant extracts as potential mosquito larvicides. Indian Journal of Medical Research 135(5), 581-598.
Giri M, Divakar K, Goli D, Dighe SB. 2009. Anti-ulcer activity of leaves of Gmelina arborea plant in experimentally induced ulcer in Wistar rats. Pharmacologyonline 1, 102.
Govindarajani M, Sivakumar R, Amsath A, Niraimath S. 2012. Larvicidal efficacy of botanical extracts against two important vector mosquitoes. European Review for Medical and Pharmacological Sciences 16, 386-392.
Gutierrez PM, Antepuesto AN, Eugenio BAL, Santos MFL. 2014. Larvicidal Activity of selected plant extracts against the dengue vector Aedes aegypti mosquito. International Research Journalof Biological Sciences 3(4), 23-32.
Hedlin PA, Holingworth RM, Masler EP, Miyamoto J, Thopson DG. 1997. Phytochemicals for Pests Control. American Chemical Society 372. https://pubs.acs.org/doi/pdf/10.1021/bk-1997-0658.ch001
Idu M, Ovuakporie- Uvo PO, Ndana RW. 2015. Preliminary Phytochemistry and In Vitro Antimicrobial Properties of the Chloroform and Ethanol Extracts of the Roots Cedrela Ordanata, Chlorophora Excelsa, and Gmelina arborea. International Journal of Analytical Pharmaceutical and Biomedical Science 4(117).
Iswarya S, Sridevi M, Mayavel A. 2017. Comparative Study on Phytochemical and Antioxidant Properties of Gmelina arborea Roxb. From four Different Geographical Regions. International Journal of Pharmaceutical and Clinical Research 9(4), 275-280. http://dx.doi.org/10.25258/ijpcr.v9i04.8533
Lawal AT. 2016. Phytochemical, Proximate and Mineral Composition of Gmelia arborea Fruits (White Teek). Fountain Journal of Natural and Applied Science 5(1), 12-18.
Lumabao JPD. 2016. Effects of Lemongrass (Cymbopogon citratus), San Francisco (Codiaeum variegatum) and Tawa- Tawa (Euphorbia hirta) on the Growth and Development of the Mosquito, Aedes aegypti, a Vector of Dengue Virus. Intel International Science and Engineering Fair (ISEF) 2016. Phoenix, Arizona, USA.
Kawamura F, Ohara S, Nishida A. 2004. Antifungal activity of constituents from the heartwood Gmelina arborea:Part 1. Sensitive antifungal assay against Basidiomycetes. Holtzforschung 58(2), 189-192. https://doi.org/10.1515/HF.2004.028
Kawamura F, Ohara S. 2005. Antifungal activity of iridoid glycosides from the heartwood of Gmelina arborea. Holzforschung 59(2), 153-155. https://doi.org/10.1515/HF.2005.023
Kulkarni YA, Addepalli V. 2009. Antidiabetic activity of aqueous extract of Gmelina arborea bark in rats. Alternative Therapies in Health and Medicine 15, 183.
Kulkarni Y, Veeranjaneyula A. 2010. Toxicological Studies of Aqueous Extract of Gmelina arborea in Rodents.Pharmaceutical biology 48(12), 1413-1420. https://doi.org/10.3109/13880209.2010.489228
Kulkarni YA, Addepalli V. 2011. Effect of Gmelina arborea extracts in STZ induced type I diabetic rats. The FASEB Journal 25(1), 805-809. https://www.fasebj.org/doi/abs/10.1096/fasebj.25.1_supplement.805.9
Kulkarni YA, Veeranjaneyulu A. 2013. Effects of Gmelina arborea extract on experimentally induced diabetes.Asian Pacific Journal of Tropical Medicine 6(8), 602-608. https://doi.org/10.1016/S1995-7645(13)60104-2
Mathivanan T, Govindarajan K, Elumalai K, Ananthan A. 2000. Mosquito Larvicidal and Phytochemical Properties of Ervantaniacoronaria Stap f. (Family Apocynaceae), Journal of Vector Borne Diseases 44, 178-180
Nayak BS, Dinda SC, Ellaiah P. 2013. Evaluation of diuretic activity of Gmelina arborea Roxb. fruit extracts. Asian Journal of Pharmaceutical and Clinical Research 6(1), 111-113.
Nayak JB. 2014. Efficacy of Crude extracts of Annona reticulata and Pongamia pinnata as larvicidal for the Management of filarial vector Culex quinquefasciatus Say Diptera: Culicidae.Universal Research Publication 4(1), 1-5.
Niang EHA, Bassene H, Fenoller F, Mediannikov O. 2018. Biological Control of Mosquito-Borne Diseases: The Potential of Wolbachia-Based Interventions in a IVM Framework. Journal of Tropical Medicine 2018, 1-15. https://doi.org/10.1155/2018/1470459
N’gaman KCC, Kabran GRM, Kadja BA, Mamyrbékova-Békro JA, Pirat JL, Lecouvey, M, Sainte-Cathérine O,Sommerer N, Verbaere A, Meudec E, Békro YA. 2014. ULPC-MS/MS phenolic quantification and in vitro anticancer potential of Gmelina arborea Roxb. (Verbenaceae). Pelagia Research Library Der Chemica Sinica 55(6), 3-17.
Offor CE. 2014. Phytochemical and proximate analyses of dry Gmelina arborea leaves. International Journal of Current Research and Academic Review, 2(12), 101-105
Okweche SI, Osai E., Umoetok A, Bassey S.2015.Maize borer damage in Nigeria’s Guinea Savanna: Timing of planting overrides effects of insecticides treatments. Revista Colombiana de Entomologia 41(2), 170-175.
Oparaeke AM. 2006. Studies on insecticidal potential of extracts of Gmelina arborea L. products for insectpests control on cowpea. 1. The legume flower bud thrips, Megalurothrips sjostedti Trybom Archives of Phytopathology and Plant Protection 39(3), 209-214. https://doi.org/10.1080/03235400500094084
Panda SK, Das D, Tripthathy NK. 2015. Evaluation of laxative and antipyretic activity of various root extracts of Gmelina arborea roxb. World Journal of Pharmacy and Pharmaceutical Sciences 4 (2), 1105-1112.
Pandey AM, Kulkarni Y. 2010. In vitro Antioxidant Activity of Different Extracts of Gmelina arborea, Free Radical Biology and Medicine 49. http://dx.doi.org/10.1016/j.freeradbiomed.2010.10.555
Patil SM, Kadam VJ, Ghosh R. 2009. In Vitro Antioxidant Activity of Methanolic Extract of Stem Bark of Gmelina arborea roxb. (Verbenaceae).International Journal of PharmTech Research 1(4), 480-1484.
Pattanayak P, Parhi PK, Mishra SK, Khandei PK. 2011. Screening of anti-diabetic activity of bark extracts of Gmelina arborea in streptozotocin-induced diabetic rats.International Journal of Pharmaceutical Sciences Review & Research 8(2), 130-132.
Pravat KP, Priyabrata P, Paresh M, Manoj KP. 2011. An In-vivo study on analgesic and antipyretic activity of bark extracts of Gmelina arborea, International Journal of Pharmaceutical Sciences Review and Research 10(2), 78-81.
Punitha D, Thandavamoorthy A, Arumugasamy K, Suresh SN, Danya U, Udhayasankar MR. 2012. Anti-Hyperlipidemic effect of ethanolic leaf extract of Gmelina arborea in streptozotocin-induced male wistar albino rats. Pharmacology and Toxicology 2(3), 46-51.
Rueda LM. 2004. Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with dengue virus transmission. Walter Reed Army Inst OF Research Washington Dc Department Of Entomology 589, 1-62. http://dx.doi.org/10.11646/zootaxa.589.1.1
Warikoo R, Wahab N, Kumar S. 2011. Larvicidal potential of commercially available pine(Pinus longifolia) and cinnamon (Cinnamomum zeylanicum) oils against an Indian strain of dengue fever mosquito, Aedes aegypti L. (Diptera: Culicidae). Acta Entomol. Sinica, 54, 793-798.
World Health Organization. 2005. Guidelines for Laboratory and Field Testing of Mosquito Larvicides.1-39.
World Health Organization. 2014. Epidemiology and burden of dengue. Focus on Dengue. 1-2
World Health Organization. 2017. Dengue Control. Retrieved from. http://www.who.int/denguecontrol/disease/en/
World Health Organization. 2019. Update on the Dengue situation in the Western Pacific Region, 2-3
Sahu R, Divakar G, Divakar K, Sharma P, Gupta SK, Rang HP, Hosny M. 2004. Evaluation of cytotoxic potential of latex of Calotropis procera and Podophyllotoxin in Allium cepa root model. Research Journal of Medicinal Plants 9(4), 501.
Saleem M, Kim HJ, Ali MS, Lee YS. 2005. An update on bioactive plant lignans. Natural product reports 22(6), 696-716. http://dx.doi.org/10.1039/B514045P
Shukla SH, Saluja AK, Pandya SS. 2010. Modulating effect of Gmelina arborea Linn. on immunosuppressed albino rats. Pharmacognosy research 2(6), 359-363. http://dx.doi.org/10.4103/0974-8490.75455
Sinha S, Dixit P, Bhargava S, Devasagayam TPA, Ghaskadbi S. 2006. Bark and fruit extracts of Gmelina arborea. Protect liver cells from oxidative stress. Pharmaceutical biology 44(4), 237-243. https://doi.org/10.1080/13880200600.713667
Sukhthankar JH, Kumar HMH, Godinho S, Kumar A. 2014. Larvicidal activity of methanolic leaf extracts of the plant, Chromolaena odorata L. (Asteraceae) against vector mosquitoes. International Journal of Mosquito Research 1(3), 33-38
Falah S, Katayama T, Suzuki T. 2008. Chemical constituents from Gmelina arborea bark and their antioxidant activity. Journal of wood science 54(6), 483-489.
Tiwary M, Naik SN, Tewary DK, Mittal PK, Yadav S. 2007. Chemical Composition and Larvicidal Activities of the Essential Oil of Zanthoxylumarmatum DC (Rutaceae) Against three Mosquito Vectors, Journal of Vector Borne Diseases 44(3), 198-204.
Valsaraja R, Pushpangadana P, Smitt UW, Adsersenb A, Nymanb U. 1997. Antimicrobial screening of selected medicinal plants in India. Ethnopharmacol 58, 75.
Vijay T, Rajan MD, Sarumathy K, Palani S, Sakthivel K. 2011. Cardioprotective, antioxidant activities and Phytochemical analysis by GC-MS of Gmelina arborea (GA) in Doxorubicin-induced myocardial necrosis in Albino rats. Journal of Applied Pharmaceutical Science 1(05), 198-204.
Jomel C. Montero (2019), Larvicidal activity of Gmelina arborea fruit extract against dengue vector Aedes aegypti mosquito; IJB, V15, N5, November, P577-586
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