Laser increased fertilization by enhance the motility of human sperm

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Research Paper 01/09/2018
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Laser increased fertilization by enhance the motility of human sperm

Aedah Z. Alkaisy
Int. J. Biosci.13( 3), 105-108, September 2018.
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To evaluate how the Low Level Laser (LLL) irradiation has influence on human sperm motility, and the velocity of human spermatozoa which was calculated by means of multiple exposure photography. 50 semen samples were chosen from men of infertility; the volunteers were of age of 25-32-year. Semen samples were stored in formalin-saline for evaluation of concentration and sperm morphology, sperm concentration greater than 200 × 106/mL, and ejaculate volume greater than 5.0 ml. Samples  were divided into five groups: a control group without laser irradiation, the other four samples groups were irradiated by an aluminum gallium indium phosphide laser diode with a wavelength of 910 nm, with 10, 12,14,18,20 min respectively, all samples were subjected for analysis using  computer-assisted sperm analyzer. It was noticed that and after each dose of laser irradiation, sperm velocity and mobility were increased. Increasing of sperm velocity was due to Laser light stimulates non-motile live spermatozoa.


Tuli RK, Singh M, Matharoo JS. 1981. Effect of different equilibration times and extenders on deep freezing of buffalo semen. Theriogenology 16, 99–104.

Abvakhitoova AK, Grigorieva LN, Parkhomenko IM. 1982. Effect of laser radiation on Chinese hamster cells cultured in vitro. Radiobiologya 22, 40-43.

Jeyendran RS, Vander-Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJ. 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Journal of reproduction and fertility 70, 219–228.

Friedmann H, Lubart R, Laulicht I, Rochkind S. 1991. A possible explanation of laser-induced stimulation and damage of cell cultures. Journal of Photochemistry and Photobiology B: Biology 11, 87-91.

Rasul Z, Anzar M, Jalali S, Ahmad N. 2000. Effect of buffering systems on post-thaw motion characteristics, plasma membrane integrity, and acrosome morphology of buffalo spermatozoa. Animal Reproduction Science 59, 31–41.

Evenson D, Jost L. 2000. Sperm chromatin structure assay is useful for fertility assessment. Methods in Cell Science  22, 169–189.

Foote RH, Kaprotht MT. 2002. Large batch freezing of bull semen: effect of time of freezing and fructose on fertility. Journal of Dairy Science 85, 453–456.

Aires VA, Hinsch KD, Mueller-Schloesser F, Bogner K, Mueller-Schloesser S, Hinsch E. 2003. In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen. Theriogenology 60, 269–279.

Muino R, Rivera MM, Rigau T, Rodriguez-Gil J, Pena AI. 2008. Effect of different thawing rates on post thaw sperm viability, kinematic parameters and motile sperm subpopulations structure of bull semen. Animal Reproduction Science 109, 50–64.

Kadirvel G, Kumar S, Kumaresan A. 2009. Lipid peroxidation, mitochondrial membrane potential and DNA integrity of spermatozoa in relation to intracellular reactive oxygen species in liquid and frozen-thawed buffalo semen. Animal Reproduction Science 114, 125–134.

Yazdi SR, Bakhshi S. 2010. Effect of 830nm diode laser irradiation human sperm motility. International journal of fertility and sterility 1, 31–32.

Akhter S, Ansari MS, Rakha BA, Ullah N, Andrabi SM, Khalid M. 2011. In vitro evaluation of liquid-stored buffalo semen at 5°C diluted in soya lecithin based extender (Bioxcell®), tris-citric egg yolk, skim milk and egg yolk-citrate extenders. Reproduction in Domestic Animals  46, 45–49.

Garg A, Kumaresan A, Ansari MR. 2015. Effects of hydrogen peroxide (H2O2) on fresh and cryopreserved buffalo sperm functions during incubation at 37 degrees C in vitro. Reprod visible and infrared laser induce stimulation and damage of cell cultures .Laser therapy 3, 39-42.

Firestone R, Esfandiari N, Moskovtse SI. Burstein E. 2012. The effect of low level laser light exposure on sperm motion characteristics and DNA damage. Journal of Andrology 33, 3.

Karu TI. 2012. Photomedicine and Laser Surgery laser in infertility treatment irradiation of oyytes and spermatozoa. Photomed Laser Surg 30, 239–241.

Sansone G, Nastri MJ, Fabbrocini A. 2000. Storage of buffalo (Bubalus bubalis) semen. Animal Reproduction Science 62, 55–76.

Dreyer TR, Siquera TD, Magrini PA, Fiorto ME. 2011. Biochemical and topological analysis of bovine sperm cells induced by low power laser irradiation. In: Medical Laser Applications and Laser Tissue Interactions, European Conferences on Biomedical Optics.