Leaf architecture of mindanao cinnamon (Cinnamomum mindanaense Elm.) in Cebu Island, Philippines

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Leaf architecture of mindanao cinnamon (Cinnamomum mindanaense Elm.) in Cebu Island, Philippines

Roi O. Engkong, Rhona Mae G. Manco, Mico D. Rosatace, Glynis Anne C. Vega, Jay P. Picardal, Esperanza Maribel G. Agoo
J. Bio. Env. Sci.14( 1), 240-256, January 2019.
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The use of leaf architecture in defining plant species has been recognized as an important tool in cinnamon taxonomy. However, available literature on the use of leaf architecture for Philippine cinnamon focused only on describing interspecies variability while intraspecies variation of economically-important Philippine cinnamon species is still unreported to date. This study assessed leaf morphological data, developed a general template of the leaves of C. mindanaense based on observable foliar characters and identified intraspecific similarities and differences of C. mindanaense leaves based on leaf architecture. We utilized 22 leaf morphological characters to examine C. mindanaense leaves (30 mature trees) obtained from (a) Nug-as, Alcoy, (b) Cansuje, Argao and (c) San Antonio, Boljoon, Cebu. Leaf architecture comparison was evaluated through quantitative comparison while pattern similarity was analyzed by Image J analysis software. Principal Component Analysis and Cluster Analysis were utilized to identify characters useful for identification and analyze extent of resemblance among morphometric data. Results show that the continuous characters (e.g. leaf length and width) of old vs young leaves were more variable compared to the discrete characters, namely: primary, secondary, tertiary, quaternary and pentary vein categories, areole shape, areole development, veinlets, blade margin, presence of petiole, venation pattern and midrib structures. The study concluded that these discrete characters may define, morphologically, C. mindanaense as a species.


Ackerly DD, Knight CA, Weiss SB, Barton K, Starmer KP. 2002. Leaf size, specific leaf area and microhabitat distribution of chaparral woody plants: contrasting patterns in species level and community level analyses. Oecologia 130, 449–457. https://doi.org/10.1007/s004420100805

Anandaraj M, Devasahayam S, Krishnamoorthy B, Mathew PA, Rema J. 2005. Cinnamon (Extension Pamphlet). Indian Institute of Spices Research, Kozhikode 1-7.

Araar H, Caboni P, Simeone V, Cavoski I. 2009. Cinnamon plant extracts a comprehensive physico-chemical and biological study for its potential use as a biopesticide. Crop protection management in Mediterranean Organic Agriculture. 39.

Barile G. 1992. Cinnamomum merkadoi. Food Crops Research Information Series on Ecosystems 3, 1-7.

Baroga JB, Buot Jr. IE. 2014. Leaf Architecture of Ten Species of Philippine Terminalia Linn. (Combretaceae). International Research Journal of Biological Sciences 3(3), 83–88.

Barthlott W. 1981. Epidermal and seed surface characters of plants: systematic applicability and some evolutionary aspects. Nordic Journal of Botany 1, 345-55. https://doi.org/10.1111/j.1756-1051.1981.tb00704.x

Barthlott W. 1984. Microstructural features of seed surfaces. In V.H. Heywood & D.M. Moore (Eds.). Current Concepts in Plant Taxonomy 95-106.

Blume CL. 1851. Laurineae. Museum botanicum Lugduno-Batavum 1(21), 322–336. Brill, Lugduni-Batavorum.

Bradshaw AD. 1965. Evolutionary significance of phenotypic plasticity in plants.  Advances in Genetics 13, 115–155. https://doi.org/10.1016/S0065-2660(08)60.048-6

Brown WH. 1921. Minor Products of Philippine Forests. Department of Agriculture and Natural Resources, Bureau of Forestry 22(2), p 417.

Bruschi P, Grossoni P, Bussotti F. 2003. Within- and among-tree variation in leaf morphology of Quercus petraea (Matt.) Liebl. natural populations. Trees 17, 164–172. https://doi.org/10.1007/s00468-002-0218-y

Buechler WK. 2004. Alternative Leaf Clearing and Mounting Procedures. 1192 E. Braemere Rd., Boise, Idaho 83702.

Cammerloher H. 1925. Die Cinnamomum-arten von Niederlandisch-Ostindien. Bulletin du Jardin Botanique Buitenzorg 3(7), 446–496.

Capuzzo JP, Rossatto DR, Franco CA. 2012. Differences in morphological and physiological leaf characteristics between T. aurea and T. impetiginosa is related to their typical habitats of occurrence. Acta Botanica Brasilica 26(3), 519-526. http://dx.doi.org/10.1590/S010233062012000300002

Celadiña DA, Buot Jr. IE, Madulid D, Evangelista TT, Tandang DN. 2012. Leaf Architecture of Selected Philippine Cinnamomum Schaeff, (Lauraceae) Species. The Thailand Natural History Museum Journal 6(2), 89-111.

Cornelissen JHC, Lavorel S, Garnier E, Diaz S, Buchmann N, Gurvich DE, Reich PB, Steege H, Morgan HD, van der Heijden MGA, Pausas JG, Poorter H. 2003. A handbook of protocols for standardized and easy measurement of plant functional traits worldwide. Australian Journal of Botany 51, 335–380. https://doi.org/10.1071/BT02124

Correa E, Green W, Jaramillo C, Jaen MCR, Salvador C, Siabatto D, Wright J. Protocol for leaf image acquisition and analysis, version 2. Retrieved as https://docplayer.net/50775252-Protocol-for-leaf-image-acquisition-and-analysis-version-2.html

Dichoso WC, Arcilla RP. 1992.  Mindanao Cinnamon (Cinnamomum mindanaense Elm.). Food Crops Research Information Series on Ecosystems 3, 22-27.

Dilcher DL. 1974.  Approaches to the identification of angiosperm leaf remains. Botanical Review 40(1), 1-157. https://doi.org/10.1007/BF02860067

Elmer ADE. 1908-1910. Lauraceae from Mt. Apo  and Mt. Giting-Giting. Leaflets of Philippine Botany 2(41), 703-728.

Ettingshausen CR. 1861. Die Blattskelete der dicotyledon. Vienna.

Gamble JS. 1912. Lauraceae: Materials for a flora of the Malayan Peninsula. Journal of the Asiatic Society of Bengal 75(2), 35–202.

Garciano DM, Nuñeza OM, Barrion-Dupo AL. 2014.Species richness of Spiders in Mt. Matutum, South Cotabato, Philippines. Journal of Biodiversity and Environmental Sciences 4(6), 214-224.

Guddadarangavvanahally K, Jayaprakasha L, Jaganmohan R Kunnumpurath KS. 2002. Chemical composition of volatile oil from Cinnamomum zeylanicum buds. Verlag der Zeitschriftfür Naturforschung 57, 990-993. https://doi.org/10.1515/znc-2002-11-1206

Hasanah M, Nuryani Y, Djisbar A, Mulyon E, Wikardi E, Asman A. 2004. Indonesian Cassia (Indonesian Cinnamon). In Cinnamon and cassia: The genus Cinnamomum. Ravindran PN, Nirmal Babu K and Shylaja M. (eds). CRC Press LLC, Florida, USA.185-198.

Hickey LJ. 1973. Classification of the Architecture of Dicotyledonous Leaves. American Journal of Botany 60(1), 17–33. https://doi.org/10.1002/j.1537-2197.1973.tb10192.x

Hickey LJ, Taylor DW. 1991. The leaf architecture of Ticodendron and the application of foliar characters in discerning its relationships. Annals of the Missouri Botanical Garden 78, 105-130. https://doi.org/10.2307/2399596

Ho KY, Hung TY. 2011. Cladistic relationships within the genus Cinnamomum (Lauraceae) in Taiwan based on analysis of leaf morphology and inter-simple sequence repeat (ISSR) and internal transcribed spacer (ITS) molecular markers. African Journal of Biotechnology 10(24), 4802–4815.

Hulshof C, Swenson NG. 2010. Variation in leaf functional trait values within and across individuals and species: an example from a Costa Rican dry forest. Functional Ecology. https://doi.org/10.1002/10.1111/j.1365-2435.2009.01614.x

Inamdar JA, Shenoy KN. 1981. Leaf Architecture in some Convolvulaceae. Phyton (Austria) 21(1), 115-125.

Jiang W, Nitin M, Jiang B, Zheng YP, Hong SS, Lu HF. 2012. Flora morphology resolves the taxonomy of Camellia L. (Theaceae) sect. Oleifera and sect. Paracamellia. Bangladesh Journal of Plant Taxonomy 19(2), 155-165.

Kostermans AJGH. 1986. Notes on Asiatic Cassine L. (Celastraceae). Gardens ’Bulletin, Singapore 39, 177-191.

Kpadehyea JT, Buot IE. 2014. Leaf architecture of two species and nine infraspecific taxa of the Philippine Mussaenda Linn. (Rubiaceae): Conservation Concerns. International Research Journal of Biological Sciences 3(10), 13–21.

Kucharczyk H, Kucharczyk M, Stanisławek K, Fedor P. 2012. Application of PCA in Taxonomy Research – Thrips (Insecta, Thysanoptera) as a Model Group, Principal Component Analysis – Multidisciplinary Applications, Dr. Parinya Sanguansat (Ed.), ISBN: 978-953-51-0129-1, InTech, Available from: www.intechopen.com/books/principal-component-analysis-multidisciplinary-applications/application-ofpca-in-taxonomy-research-thrips-insecta-thysanoptera-as-a-model-group

Kulkarni AH, Rai HM, Jahagirdar KA, Upparamani PS. 2013. A Leaf recognition technique for plant classification using RBPNN and Zernike Moments. International Journal of Advanced Research in Computer and Communication Engineering 2(1), 984-988.

KusI J. 2013. Variations in phenotypic plasticity and fluctuating asymmetry of leaf morphology of three Quercus (Oak) species in response to environmental factors. Electronic Theses and Dissertations. Paper 1160. http://dc.etsu.edu/etd/1160

Lach DF, Van Kley EJ.  1998. Asia in the Making of Europe, Volume III: A Century of Advance book 3: Southeast Asia. 1027 E 57th St, Chicago, IL 60637, United States: University of Chicago Press. 578 p.

Lambrecht SC, Dawson TE. 2007. Correlated variation of floral and leaf traits along a moisture availability gradient. Oecologia 151, 574–583. https://doi.org/10.1007/s00442-006-0617-7

Laraño AAP, Buot Jr IE. 2010. Leaf Architecture of Selected Species of Malvaceae sensu APG and Its Taxonomic Significance. Philippine Journal of Systematic Biology IV: 21–54.

Leaf Architecture Working Group. 1999. Manual of Leaf Architecture – morphological description and categorization of dicotyledonous and net-veined monocotyledonous angiosperms 65 p.

Lorea-Hernandez FG. 1996. A Systematic Revision of the Neotropical Species of Cinnamomum Schaeffer (Lauraceae). Unpublished PhD Dissertation, University of Missouri- St. Louise, USA., p 269.

Lu H, Jiang W, Ghiassi M, Lee S, Nitin M. 2012. Classification of Camellia (Theaceae) Species Using Leaf Architecture Variations and Pattern Recognition Techniques. PLoS ONE 7(1), e29704. https://doi.org/10.1371/journal.pone.0029704

Mabberley DJ. 2008. Mabberley’s plant-book: A portable dictionary of plants, their classifications and uses, third edition. Cambridge University Press

Meisner CF. 1864. Lauraceae. In: De Candolle A (ed), Prodromus systematis naturalis regni vegetabilis 15(1), 1–260. Sumptibus Victoris Masson et Filii, Paris.

Miquel FAW. 1858. Flora van Nederlandsch Indie 1(2). Van der Post, Amsterdam.

Miquel FAW. 1864. Cinnamomum. Annales Musei Botanici Lugduno-batavi. 1:257–270. Van der Post, Amsterdam.

Moore BR, Narkkong NA, Moore T, Lutat P. 2008. Epicuticular leaf architecture confirms a new Smilax species (Smilacaeae) from Northeast Thailand. Science Asia 34, 103-106.

Navas ML, Garnier E. 2002. Plasticity of whole plant and leaf traits in Rubiaperegrina in response to light, nutrient and water availability. ActaOecologica 23, 375–383. https://doi.org/10.1016/S1146-609X(02)01168-2

Nirmal Babu K, Ravindran PN, Shylaja M. 2004. Camphor Tree. In Cinnamon and cassia: The genus Cinnamomum. Ravindran PN, Nirmal Babu K and Shylaja M. (eds). CRC Press LLC, Florida, USA. pp. 211-238.

Nurdjannah N. 1992. Processing of the genus Cinnamomum. Proceedings of the Conference on Research and Development of Biological Resources Center for Biology, 321-330.

Premoli AC. 2008. Leaf architecture of South American Nothofagus (Nothofagaceae) using traditional and new methods in morphometrics. Botanical Journal of the Linnean Society 21, 25–40. https://doi.org/10.1111/j.1095-8339.1996.tb00743.x

Pacheco-Trejo J, Terrazas T, Ochoterena H. 2009. Leaf architecture of the genus Didymaea Hook. f. (Rubiaceae). Plant systematics and Evolution 281 (1), 137-149. https://doi.org/10.1007/s00606-009-0195-3

Peppe DJ, Royer DL, Cariglino B, Oliver SY,  Newman S, Leight E, Enikolopov G, Fernandez-Burgos M, Herrera F, Adams JM, Correa E, Currano ED, Erickson JM, Hinojosa LF, Iglesias A, Jaramillo CA, Johnson KR, Jordan GJ, Kraft N, Lovelock EC, Lusk CH, Niinemets, Peuelas J, Rapson G, Wing SL, Wright IJ. 2011. Sensitivity of leaf size and shape to climate: global patterns and paleoclimatic applications. New Phytologist 190, 724-739. https://doi.org/10.1111/j.1469-8137.2010.03615.x

Picardal J. 2017. Systematics, ecology and ethnobotany of cinnamon (Cinnamomum Schaeffer) in the Philippines. Unpublished PhD Dissertation in Biology, De La Salle University Manila. 264 p.

Ponton S, Dupouey J, Dreyer E. 2004. Leaf morphology as species indicator in seedling of Quercus robur L. and Q. petreae (Matt.) Liebl.: modulation by irradiance and growth flush. Annals of Forestry Science 61, 73-80.

Priya J, Maridass M. 2008 Inter-species relationship of Cinnamomum species using RAPD marker analysis. Ethnobotany Leaflets 12, 476- 480.

Pulan DE, Buot Jr. IE. 2014. Leaf architecture of Philippine Shorea species (Dipterocarpaceae). International Research Journal of Biological Sciences 3(5), 19–26.

Pyakurel A, Wang J. 2013. Leaf morphological variation among paper birch (Betula papyrifera Marsh.) genotypes across Canada. Open Journal of Ecology 3, 284-295.

Radford AE, Dickinson WC, Massey JR, Bell CL. 1974. Vascular plant systematics. Harper & Row Publishers, New York.

Ranatunga J, Senanayake U, Wijesekera R. 2004. Cultivation and Management of Cinnamon. In Cinnamon and Cassia: The genus Cinnamomum. N.W. Corporate Blvd., Boca Raton, Florida. CRC  Press. 121–129.

Ravindran PN, Nirmal Babu K. 2004. Cinnamon and Cassia: The genus Cinnamomum. N.W. Corporate Blvd., Boca Raton, Florida. CRC Press. 379.

Ravindran PN, NirmalBabu K, Shylaja M. 2004. Cinnamon and Cassia. N.W. Corporate Blvd., Boca Raton, Florida: CRC Press, p 379.

Ridley HN. 1924. The Flora of the Malay Peninsula 3. Reeve, London.

Rohwer JG. 1993. Lauraceae.  In Kubitzki, K., J. G. Rohwer, and V. Bittrich. The Families and Genera of Vascular Plants, Springer Verlag, Berlin 2, p 366–391.

Roth-Nebelsick A, Uhl D, Mosbrugger V, Kerp H. 2001. Evolution and function of leaf venation architecture: a review. Annals of Botany 87, 553-566. https://doi.org/10.1006/anbo.2001.1391

Russell P, Hertz P, McMillan B. 2013. Biology: The Dynamic Science. Cengage Learning:, p 1456.

Santos PK. 1930. Leaf and bark structure of some cinnamon trees with special reference to the Philippine species. Philippine Journal of Science 43, 305-365.

Scheiner SM. 1993. Genetics and evolution of phenotypic plasticity. Annual Review of Ecological Systematics 24, 35–68. https://doi.org/10.1146/annurev.es.24.110193. 000343

Sivarajan VV. 1991. Introduction to the Principles of Plant Taxonomy. Cambridge University Press 2,  292 pages.

Stace CA. 1970. Anatomy and taxonomy in Juncus subgenus Genuini. Botanical Journal of the Linnaean Society 63(11), 75-81.

Varghese TM. 1997. Introduction to the anatomy of angiosperm. Allied Publishers Limited. New Delhi, India 92 p.

Vasco A, Thadeo M, Conover M, Daly DC. 2014. Preparation of samples for leaf architecture studies: A method for mounting cleared leaves. Applications in Plant Sciences 2(4), 1400038. https://doi.org/10.3732/apps.1400038

Vijayan K, Ajithan Thampuran R. 2004. Pharmacology and toxicology of cinnamon and cassia. In Cinnamon and Cassia: The genus Cinnamomum. N.W. Corporate Blvd., Boca Raton, Florida. CRC Press. 259–284.

Walter KS, Gillet HJ. 1998. 1997 IUCN Red List of Threatened Plants. Gland, Switzerland: IUCN- The World Conservation Union. 936 p.

Wells CL, Pigliucci M. 2000.  Adaptive phenotypic plasticity: the case of heterophylly in aquatic plants. Perspectives in Plant Ecology, Evolution and Systematics 3(1), 1–18.

Wuu-Kuang S. 2011. Taxonomic revision of Cinnamomum (Lauraceae) in Borneo. Blumea 56, 241– 264.

Xu F, Guo W, Xu W, Wang R. 2008. Habitat effects on leaf morphological plasticity in Quercusacutissima. Acta Biologica Cracoviensia Series Botanica 50(2), 19-26.