home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Low cost alternatives for micro propagation of Moringa (Moringa stenopetala L.)

Paper Details

Research Paper 05/08/2023
Views (1096) Download (62)
current_issue_feature_image
publication_file

Low cost alternatives for micro propagation of Moringa (Moringa stenopetala L.)

Endris Said, Temesgen Menamo, Gizachew Haile
Int. J. Biosci.23( 2), 157-163, August 2023.
Certificate: IJB 2023 [Generate Certificate]
Key: Enset starchIn vitro propagationMoringaShoot tip cultureTable sugar

Abstract

Moringa (Moringa stenopetala) is widely cultivated in southern Ethiopia for its nutrition and medicinal value. Micro propagation to preserve the plant becomes costly due to the tissue culture media cost. The aim of the study was to evaluate low cost alternative media components for micro propagation of Moringa (Moringa stenopetala). Enset starch as gelling agent and table sugar as a carbon source were used as a low cost alternative media components for moringa culture initiation and multiplication using shoot tip. The highest mean numbers of shoots per explants (6.94±0.26), shoot length (4.88±0.16cm) and leaf number (17.07±0.86) were obtained in media containing 80g/l enset starch as an alternative gelling agent. The response of the explant for 30g/l sucrose and table sugar were not significantly different. Utilization of enset starch as gelling agent reduced the gelling agent cost by 70% while table sugar reduced sucrose cost by 95%. The micro propagation rates under the experimental conditions using the low cost media alternative were equal to or higher than the conventional media components. Therefore, the obtained result can be used in commercial micropropagation of moringa. However, studies on transparency of enset starch gel at higher concentration could be explored for wider application in tissue culture laboratories.

VIEWS 190
Cover PDF

Abebaw YM, Tobiaw DC, Abate BA, Eshete BK, Seymour SK, Tesfaye K. 2021. Plant tissue culture research and development in Ethiopia: A case study on current status, opportunities, and challenges. Advances in Agriculture 1-12. https://doi.org /10.1155/2021/9979549.

Amare K, Dugassa G. 2022. Plant tissue culture challenges in Ethiopia and alternative options for low-cost media. F1000Research 11, 828.

Amlesom WS, Mehari T, Saleh BK. 2021. Evaluation of different starches as gelling agents for micropropagation of potato. The Journal of Agricultural Science 13(5), 144.

Ayelign M, Biruk A, Elias G, Tewodros T. 2012. Micro-propagation of Vanilla planifolia Using enset (Ensete ventricosum (Welw, cheesman)) starch as a gelling agent. Current Research Journal of Biological Science 4, 519-525.

Ayenew B, Mengesha A, Tadesse T, Gebre Mariam E. 2012. Ensete ventricosum (welw) Cheesman: A cheap and alternative gelling agent for pineapple (Ananas comosus var. Smooth cayenne) in vitro micropropagation. Journal of Microbiology, Biotechnology and Food Sciences 2, 640-652

Cardoso C. 2019. Silver nitrate enhances in vitro development and quality of shoots of Anthurium andraeanum Jean. Scientia Horticulture 253, 358-363. https://doi.org/10.1016/j.scienta.2019.04.054.

Chen C. 2016. Cost analysis of plant micropropagation of Phalaenopsis. Plant Cell, Tissue and Organ Culture 126(1), 167-175. https://doi.org /10.1007/s11240-016-0987-4

Daud N, Mat Tha R, Noor N, Alimon H. 2011. Potential of alternative gelling agents in media for in vitro micro-propagation of Celosia sp. International Journal of Botony 7, 183-188. http://doi.org /10.3923/ijb.2011.183.188

Demo P, Kuria P, Nyende AB, Kahangi EM. 2008. Table sugar as an alternative low cost medium component for in vitro micro-propagation of potato (Solanum tuberosum (L.)). African Journal of Biotechnology 7, 2578-2584.

Dhawale RN, Patwari LV, Sharma KM, Bharose AA. 2021. Admixture of Isubgol husk together with agar as gelling agent for sugarcane callus induction. Pharma Innovation 10(8), 675-680.

Fahey JW. 2005. Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part1. Trees for life Journal 1, 1-15.

Getnet B, Bantte K, Diro M. 2016. Table sugar as low cost option and its concentration effect on shoot multiplication of sugarcane (Saccharum officinarum L.) genotypes. Advances in Crop Science and Technology 04(248), 2.

Jabeen T, Kausar R, Shah AH. 2017. Development of low cost protocol for micropropagation of Solanum tuberosum (L.). Bangladesh Journal of Botany 46, 1341-1345.

Khadiga G, Rasheid S, Mutasim M. 2009. Effect of cultivar and growth regulator on in vitro micropropagation of potato (Solanum tuberosum L). American-Eurasian Journal of Sustainable Agriculture 3, 487-492

Kumar M, Pandey S, Kumar V, Yadav L, Kumar J, Singh BP. 2021.Studies on the effect of different gelling agents for establishment of Callus strawberry (Fragaria ananassa Duch.) culture under in vitro condition. International Journal of Chemical studies 9, 253-255

Lebedev V, Arkaev M, Dremova M, Pozdniakov I, Shestibratov K. 2019. Effects of growth regulators and gelling agents on ex vitro rooting of raspberry. Plants 8, 3. http://doi.org/ 10.3390/plants8010003.

Mahalakshmi R, Vineetha MV, Warrier RR. 2018. Optimising gelling agents, light source and after-care to commercialise teak tissue culture. Plant Tissue Culture & Biotechnology 28, 13-24. http://doi.org/10.3329/ptcb.v28i1.37194.

Mengesha A, Ayenew B, Gebremariam E, Tadesse T. 2012. Micro-propagation of Vanilla planifolia using Enset (Ensete ventricosum Welw, cheesman) starch as a gelling agent. Current Research Journal of Biological Sciences 4(4), 519-525.

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15, 473-497.

Nery LA, Batista DS, Rocha DI, Felipe SHS, Queiroz MCQ, Silva PO, Ventrella MC, Otoni WC. 2021. Leaf development and anatomy of in vitro grown Polygala paniculata L. are affected by light quality, gelling agents, and sucrose. Vegetos 34, 19-28. https://doi.org/10.1007/s42535-021-00192-3.

Nouman W, Siddiqui M, Basra S, Khan R, Gull T, Olson M, Munir H. 2012. Response of Moringa oleiferato saline conditions. International Journal of Agriculture Biology 14, 757-762.

Ogero KO, Gitonga NM, Mwangi M, Ombori O, Ngugi M. 2011. A low-cost medium for sweet potato micropropagation. African Crop Science Conference Proceeds 10, 57-63.

Pant M. 2016. A minimal cost micropropagation protocol for Dianthus caryophyllus (L). a commercially significant venture. Indian Journal Experimental Biology 54, 203-211.

Saini R, Shetty N, Giridhar P, Ravishankar G. 2012. Rapid in vitro regeneration method for Moringa oleifera and performance evaluation of field grown nutritionally enriched tissue cultured plants. 3 Biotechnology 2, 187–192. https://doi.org/10.1007 /s13205-012-0045-9.

SAS Inistitute. 2008. SAS/STAT 9.1 User’s Guide the Reg Procedure:(Book Excerpt), SAS Institute.

Sharifi A, Moshtaghi N, Bagheri A. 2016. Agar alternatives for micropropagation of African violet (Saintpaulia ionantha). African Journal of Biotechnology 9, 9199-9203.

Silvosa-Millado CSC, Dejan ACM, Asumbrado MJLC, Delima AGD, Novero AU. 2020. Alternative materials for plumule initiation and callus induction of coconut (Cocos nucifera (L).) cv. Laguna Tall. Banwa B 15, art042

Twaij BM, Jazar ZH, Hasan M. 2020. Trends in the use of tissue culture, applications and future aspects. International Journal of Plant Biology 11(1), 8385. https://doi.org/10.4081/pb.2020.8385.

Umbreen S, Muhammad J, Saeed A, Faisal S. 2014. Optimization of the micro-cloning system of threatened Moringa oleifera Lam. Pakistan Journal of Agriculture Science 51, 449-457.

Vettorazzi RG, Carvalho VS, Sudré CP, Rodrigues R. 2017. Developing an in vitro optimized protocol to sweet potato landraces conservation. Acta Scientiarum. Agronomy 39, 359-367. https://doi.org/10.4025/actasciagron.v39i3.

Submit Manuscript