Macroscopic and microscopic study of the embryonic development of the desert locust Schistocerca gregaria (Forskal, 1775) (Orthoptera: Acrididae) in laboratory

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Macroscopic and microscopic study of the embryonic development of the desert locust Schistocerca gregaria (Forskal, 1775) (Orthoptera: Acrididae) in laboratory

Ouali-N’goran San-Whouly Mauricette, D’Almeida Marie-Anne, Kouassi Kouassi Philippe, Tano Yao, Fouabi Kouahou
Int. J. Biosci.3( 12), 97-104, December 2013.
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Schistocerca gregaria (Forskal, 1775) is a serious pest of crops. The knowledge of its biology can contribute to the integrated pest management against locusts. In laboratory conditions (32 ± 2.9 °C and 70 ± 5.3% RH), the duration of the embryonic development of Schistocerca gregaria is 12.63 ± 0.55 days. The macroscopic study showed that the bright yellow egg at laying, changes to orange 2-3 hours after laying; then to Brown 1-2 days later and eventually becomes clear at the end of development. These color changes are accompanied by weight gain of 8.3-20 mg at the end of development. Observation by transparency of rudiments of organs was made from the 5th day. These transformations correspond to the stages of segmentation, gastrulation and organogenesis. These three stages interfere and overlap.Histological investigations reveal partial and superficial segmentation, characterized by an early Karyiokinesis followed by cytokinesis. After one hour and thirty minutes the periblastula is formed. Gastrulation is initiated immediately, with movements of cell proliferation, deployment, embolism and delamination leading to the formation of the embryonic layers within 24 hours. Clusters of cells from which will derive rudiments of organs are observed on sections of eggs of two days. Morphogenesis begins with cephalization with the formation of the optic lobes, then the thorax and abdomen. The whole morphogenesis lasts about ten days. Knowledge of the duration and timing of embryonic development will allow to provide the best times and the means of intervention to contribute to the integrated pest management (IPM) against locusts.


Ball EE, HO RK, Goodman CS. 1985. Muscle development in the grasshopper embryology I. Muscles, nerves and apodemes in the metathoracic leg. Developmental Biology 111(2), 383-98.

Bentley D, Keshishian H, Shankland M, Toroian-Raymond A. 1979. Quantitative staging of embryonic development of the grasshopper, Schistocerca nitens. Journal of Embryology and Experimental Morphology 54, 47-74.

Shepherd D, Laurent G. 2004. Embryonic development of a population of spiking local interneurones in the locust (Schistocerca gregaria). Journal of Comparative Neurology 319 (3), 438-453.

Borror DJ, De Long DM, Tripplehorn CA. 1981. An introduction to the study of Insects. Saunders College publishing, Philadelphia, New York 5, 72-153.

Campos-Ortega JA, Hartensein V, 1997. The embryonic development of Drosophila melanogaster. 2, 56-345 p. Springer, Berlin.

Dearden PK, Akam M. 2001. Early embryo patterning in the grasshopper, Schistocerca gregaria: wingless, decapentaplegic and caudal expression. Development 128(18), 3435-3444.

Ould El Hadj MD, Tankari Dan-Badjo A, Halouane F. 2004. Etude du cycle biologique de Schistocerca gregaria (Forskål, 1775) sur chou (Brassica oleracea) en laboratoire. Courrier du Savoir 5, 17-21.

Duraton JF, Lecoq M. 1990. Le criquet pèlerin au Sahel. Collection Acridologie Opérationnelle CIRAD/ PRIFAS, France 6, 11-153.

FAO. 2012. La crise du Sahel / la crise alimentaire et nutritionnelle du sahel : l’urgence d’appuyer la résilience des populations vulnérables. 4-11.

Franquinet R, Foucrier J. 2003. Embryologie descriptive [Edition Dunod] 2, 43-52.

Harrat A, Petit D. 2009. Chronologie du développement embryonnaire de la souche « Espiguette » avec ou sans diapause de Locusta migratoria Linnaeus (Orthoptera : Acrididae). Comptes Rendus Biologies 332(7), 613-622.

Ho K, Dunin-Borkowski OM, Akam M. 1997. Cellularization in locust embryos occurs before blastoderm formation. Development 124, 2761-2768. Great Britain © The Company of Biologists.

Hountondji HY-C. 2008. Dynamique environnementale en zones sahélienne et soudanienne de l’Afrique de l’Ouest : Analyse des modifications et évaluation de la dégradation du couvert végétal. Thesis of Doctorat, University of Liège, Belgique, 74-131.

Hunter-Jones C. 1964. Egg development in the desert locust (Schistocerca gregaria Forskäl) in relation to the availability of water. Proceeding Research Entomology Society London 39, 25-33.

Johannsen OA, Butt FH. 1950. Embyology of Insects and Myriapods. Mc Graww-Hill, New-York. In: The Insects Structures and function. The english Universities Press LTD. St. Paul’s House Warwick Lane London, 345-368.

Lamotte M, L’héritier P. 1969. Les étapes du développement embryonnaire des Métazoaires In: Biologie générale III. Développement et morphogenèse [Edition Doin Deren et Cie] 15-80.

Lecoq M, Mestre J. 1988. La surveillance des sauteriaux du Sahel. Collection Acridologie Opérationnelle CILSS-DFPV, Niamey, 2, 6-52.

Martoja R, Martoja-Pierson M. 1967. Initiation aux techniques de l’histologie animale [Edition Masson et Cie, Paris] 140-345.

Raccaud- Schoeller J. 1980. Les Insectes, Physiologie, Développement. [Edition Masson, Paris] 125-200.

Simpson SJ, Mc Caffery AR, Hägele BF. 1999. A behavioural analysis of phase change in the desert locust. Biological Review of the Cambridge Philosophical Society. 74, 461-480.

Thompson KJ, Siegler MS. 1993. Development of segment specificity in identified lineages of the grasshopper CNS. The Journal of Neuroscience, 13 (8), 3309-3318.