Mammary tumor in mammals and the risk factors: comparative clinical pathological study

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Research Paper 01/05/2019
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Mammary tumor in mammals and the risk factors: comparative clinical pathological study

Huda Hameed, Kadhim Alabbody
J. Bio. Env. Sci.14( 5), 83-92, May 2019.
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The samples of the study included three species of mammals, including: ten female dogs, five women and one Holstein Friesian cow. All of them had a lump on their mammary glands. The study aimed to determine the susceptibility of these species to mammary tumors. Case history and clinical findings were recorded for each individual from whom specimens were collected. The biopsies were subjected to H & E staining for histopathological examination as well as immunohistochemistry technique for BRCA1and Ki67 level determination. On histopathological slides, 8 out of 10 of dogs showed carcinoma, which was classified into adeno, lipo, solid or inflammatory, while the other two cases had benign adenoids. The cases of women revealed different patterns: one had ductal carcinoma in situ, another one with lobular carcinoma in situ, two cases had fibroadenoma and the last case showed a cyst. In the cow, the masses were founded in the teat of the left front quarter of the mammary gland with enlargement of local and sub scapular lymph nodes. In conclusion, female carnivores represented by female dogs were more sensitive to catch a mammary tumor than omnivores represented by women, while herbivores represented by a cow were the most resistant. Therefore, these data recommend performing more studies that focus on the variability among animal species in order to reduce the incidence of mammary tumors in mammals especially humans and dogs.


Alabbody HH, Ban SAN, Kadhim KhH. 2018. Applying food frequency questionnaire to evaluate the dietary pattern and life style on women with breast cancer, Fac Med Baghdad 60(2), 119-125.

Alabbody HH, Lafta IJ. 2018. Clinical Pathology and immuno-histochemistry of mammary tumours from military and pet dogs in Iraq, Online Journal of Veterinary Research, Volume 22(7), 547-560.

Alabbody HHK. 2018. Bovine leukosis and the possibility to cause cancer in humans: A scientific review, The Iraqi Journal of Veterinary Medicine 42(1), 52-60.

Allred DC, Harvy JM, Berardo M, Clark GM. 1998. Prognostic and predictive factors in breast cancer by immunohistochemical analysis. Mod Pathol 11(2), 155-168.

Amjeed AKH. 1994. Serological study for bland IBR viruses in and around Baghdad area by ELISA, Iraq Vet. Med 18, 82-87.

Bastianello SS. 1982a. A survey on neoplasia in domestic species over a 40-year period from 1935 to 1974 in the Republic of South Africa, I. Tumours occurring in cattle. Onderstepoort J. Vet. Res 49(4), 195-204.

Carey LA, Dees EC, Sawyer L, Gatti L, 2007. The triple negative paradox: primary tumor chemosensitivity of breast cancer subtypes. Clinical cancer research: an official journal of the American Association for Cancer Research 13(8), 2329-34.

Chrisp CE, Spangler WL. The malignant canine mammary tumor as a model for the study of human breast cancer. In: Shifrine M, Wilson FD, editors. The Canine as a Biomedical Research Model: Immunological, Hematological and Oncological Aspects. Oak Ridge (TN): Technical Information Center/U.S. Department of Energy (Dist. by National Technical Information Service/U.S. Department of Commerce, Springfield, VA) 1980, 331-49.

Cohen D, Reif JS, Brodey RS, Keiser H. 1974. Epidemiological analysis of the most prevalent sites and types of canine neoplasia observed in a veterinary hospital. Cancer Res 34, 2859-68.

Cotran R, Kumar V, Robbins SL, Schoen FJ. 1994. editors. Pathologic Basis of Disease. 5th ed Philadelphia: WB Saunders.

Dorn CR, Taylor DO, Schneider R. 1968. Survey of animal neoplasms in Alameda and Contra Costa Counties, California: II. Cancer morbidity in dogs and cats from Alameda County. J Natl Cancer Inst. 40, 307-18.

Egenvall A, Bonnett BN, Ohagen P, Olson P, Hedhammar A, von Euler H. 2005 Incidence of and survival after mammary tumors in a population of over 80,000 insured female dogs in Sweden from 1995 to 2002. Prev Vet Med 69, 109-27.

Enger SM, Ross RK, Henderson B, Bernstein L. 1997. Breast feeding history, pregnancy experience and risk of breast cancer. Br. J. Cancer 76(1), 118-123.

Fidler IJ, Brodey RS. 1967. The biological behavior of canine mammary neoplasms. J Am Vet Med Assoc; 151, 1311-18.41.

Ford TS, Ross MW, Acland HM, Madison JB. 1989. Primary teat neoplasia in two yearling heifers. J. Am. Vet. Med. Assoc 195, 238-239.

Frese K. 1985. Comparative pathology of breast tumors in domestic animals, VerhDtsch Ges Pathol 69, 152-70.

Gerdes J, Lemke H, Baisch H. 1984. Cell cycle analysis of a cell proliferation associated human nuclear antigen defined by the monoclonal antibody Ki‐67. J Immunol 133, 1710- 15.

Gilbertson SR, Kurzman ID, Zachrau RE, Hurvitz AI, Black MM. 1983. Canine mammary epithelial neoplasms: biologic implications of morphologic characteristics assessed in 232 dogs. Vet Pathol 20, 127-42.

Guoxin R, Xuhui M, Yunteng W 2017. Ki67 Proliferation Index as a Histopathological Predictive and Prognostic Parameter of Oral Mucosal Melanoma in Patients without Distant Metastases, Journal of Cancer 8(18), 3828-3837. DOI: 10.7150/jca.20935 Research Paper Ki67.

Hasso SA, Zenad MM, Abdulla M. 1994. Preliminary diagnosis of bovine viral leucosis in Iraq, Bovine Pract 28, 164.

Khudhair YI, Hasso SA, Yaseen NY, Al-Shammari AM. 2016. Serological and molecular detection of bovine leukemia virus in cattle in Iraq, Emerging Microbes Infect 56(5), 1-6.

Lindblad-TK, Wade CM, Mikkelsen TS, 2005. Genome sequence, comparative analysis and haplotype structure of the domestic dog. Nature 438, 803-19.

McPherson CM, Steel JM, Dixon K. 2000. ABC of Breast Diseases. Breast cancer -epidemiology, risk factors, and genetics. Clinical review. BMJ 321(9), 624-628.

Megalhaes GM, Silveira ACT, Maunari DP, Alessi AC. 2012. Behavior of CD44 receptors in mammary tumors of dogs. Open Journal of Veterinary Medicine 2, 48-51.

Moulton JE, Taylor DO, Dorn CR, Andersen AC. Canine mammary tumors 1970 Vet Pathol 7, 289-320.

Munson L, Moresco A. 2007. Comparative pathology of mammary gland cancers in domestic and wild animals. Breast Disease 28, 7-21.

Murphy P. 1992. Mammary carcinoma with peritoneal metastasis in cow. Vet. Pathol 129, 552-553.

Ohfuji S. 2012. Case report. Secretory carcinoma of the mammary gland in an 8-year-old Holstein-Friesan dairy cow. Veterinary Quarterly 32( 2), 113-115.

Pandey GS, Sharma RN, Chizyuka HGB. 1984. Study of bovine neoplasms in Zambia, Bull Anim Hlth Prod Afr 32, 289-291.

Patricio R, Malin M, Tara B, 2009. Mammary Tumor Development in Dogs is associated with BRCA1 and BRCA2, Cancer Res 69(22), 8770-4.

Pattanayak S. 2004. Autohemotherapy in Bovine Papillomatosis. Intas Polivet 5(1), 16-17.

Perez AMD, Pena L, Castillo N, Nieto AI. 2000. Factors influencing the incidence and prognosis of canine mammary tumors, J. Small Anima Pract 41, 287-291.

Povey RC, Osborne AD. 1969. Mammary gland neoplasia in the cow. Path. Vet 6, 502-512.

Queiroga FL, Raposo T, Carvalho MI, Prada J, Pires I. 2011. Canine mammary tumours as a model to study human breast cancer: most recent findings. In Vivo 25, 455-65.

Raposo TP, Arias-Pulido H, Chaher N, 2017. Comparative aspects of canine and human inflammatory breast cancer. Seminars in Oncology 44(4), 288-300.

Rismanchi S, Yadegar O, Muhammadnejad S, Amanpour. 2014. Expression of vimentin filaments in canine malignant mammary gland tumours: A simulation of clinicopathological features of human breast cancer. Biomed Rep 2, 725- 28.

Schneider R, Dorn CR, Taylor DO, Factors. 1969. Influencing canine mammary cancer development and postsurgical survival. J Natl Cancer Inst 43, 1249-61.

Sonja PM, Peter D. 2013. Mammary tumors in ruminants Acta argiculturae Slovenica 102(2), 83-86.

Sorenmo KU, Kristiansen VM, Cofone MA, 2009. Canine Mammary gland tumours; a histological continuum from benign to malignant; clinical and histopathological evidence. Vet Comp Oncol 7, 162-172.

Sutter NB, Eberle MA, Parker HG, 2004. Extensive and breed-specific linkage disequilibrium in Canis familiaris. Genome Res 14, 2388-96.

Sweet WW, Matthews CA, Graves RR. 1940. Extreme rarity of cancer in cow’s udder. J. Dairy Sci 23, 437-446.

Voduc KD, Cheang MC, Tyldesley S, Gelmon K. 2010. Breast cancer subtypes and the risk of local and regional relapse. Journal of clinical oncology: official journal of the American Society of Clinical Oncology 28(10), 1684-91.

Walsh, King MC. 2007. Ten genes for inherited breast cancer. Cancer Cell 11, 103-5.

World Cancer Report. 2014. International Agency for Research on Cancer, World Health Organization; ISBN 978-92-832-0432-9.

Yang Q, Sakurai T, Mori I, Yoshimura G. 2001. Prognostic significance of brca 1 expression in Japanese sporadic breast carcinoma. American cancer society 92(1), 54-60.

Yousif AA. 1997. Serological study of bovine leukosis in Iraq local and imported cow. Iraqi J. Vet. Med 21(1), 137-142.