Marker-assisted selection and pyramiding of I1 and Ph3 genes for multiple disease resistance in tomato through PCR analysis

Paper Details

Research Paper 01/09/2016
Views (377) Download (16)

Marker-assisted selection and pyramiding of I1 and Ph3 genes for multiple disease resistance in tomato through PCR analysis

Kehkashan Akbar, Fida Muhammad Abbasi, Muhammad Sajid, Mehboob Ahmad, Zaheer Ullah Khan, Aziz-Ud-Din, Hamid Ali
Int. J. Biosci.9( 3), 108-113, September 2016.
Certificate: IJB 2016 [Generate Certificate]


Fusarium wilt and late blight are the most devastating diseases of tomato that causes significant yield loses all over the world. Genetic host resistance is the most effective way to control these problems. Marker assisted selection (MAS) was carried out to screen 46 genotypes of tomato for the presence of Fusarium wilt resistance gene (I1) and late blight resistance gene (Ph3). For this purpose allele specific SSR marker Tom-144 and SCAR marker SCU602 were used that are tightly linked to I1 and Ph3 genes, respectively. In the present study 17 genotypes showed the presence of I1 gene and 13 genotypes showed the presence of Ph3 gene while five genotypes were found to possess both I1 and Ph3 genes. Thus we have successfully pyramided I1 and Ph3 genes into five tomato lines that are accessions 1008, 017878, 017868, 0101 and 1002.  These genotypes are highly resistant to both Fusarium wilt and late blight of tomato and thus should be released as resistant inbred lines for general cultivation by farmers.


Abdel-Monaim MF. 2012. Induced systemic resistance in tomato plants against Fusarium wilt disease. International Research Journal of Microbiology 3(1), 14-23.

Ali H, Abbasi FM, Ahmad H, Aziz-Ud-Din, Abzar, Khan A, Khan MA, Irfanullah, Zeb A, Sarwar A. 2016. Identification of fragrance gene in some elite advance lines of rice cultivated in foothills of the Himalayas. International Journal of Biosciences 8(1), 47-54.

Foolad MR, Panthee DR. 2012. Marker-assisted selection in tomato breeding. Critical Reviews in Plant Sciences 31, 93-123.

Liggitt J, Jenkinson P, Parry DW. 1997. The role of saprophytic microflora in the development of Fusarium ear blight of winter wheat caused by Fusarium culmorum. Crop Protection 16, 679-685.

Lumsden RD, Locke JC. 1989. Biological control of damping-off caused by Pythium ultimum and Rhizoctonia solani with Gliocladium virens in soilless mix. Phytopathology 79(3), 361-366.

Parmar P, Sudhir A, Preethi R, Dave B, Panchal K, Subramanian RB, Patel A, Kathiria KB. 2013. Identification of a SSR marker (TOM-144) linked to Fusarium wilt resistance in Solanum lycopersicum. American Journal of Molecular Biology 3(4), 241.

Singh RS. 2005. Plant Diseases. New Delhi: Oxford and IBH 547 Publication Co. Pvt. Ltd.

Song F, Goodman RM. 2001. Molecular biology of disease resistance in rice. Physiological and Molecular Plant Pathology 59(1), 1-11.

Stevens MA, Rick CM. 1986. Genetics and breeding. In The tomato crop. Springer Netherlands. 35-109 p.

Truong HTH, Tran HN, Choi HS, Park PH, Lee HE. 2013. Development of a co-dominant SCAR marker linked to the Ph-3 gene for Phytophthora infestans resistance in tomato (Solanum lycopersicum). European Journal of Plant Pathology 136(2), 237–245.