Paper Details

Methylation status of CDH1 promoter and E-cadherin expression in colorectal cancer

Jamshid Mehrzad, Mahdieh Mohammaditabr, Zohreh Ebrahimi

DOI: https://dx.doi.org/10.12692/ijb/4.10.13-21

Int. J. Biosci. 4(10), 13-21. May, 2014. (PDF)

Abstract:

The E-cadherin plays a crucial role in epithelial cell cell adhesion and in the maintenance of a tissue. In colorectal cancer (CRC), changes in E-cadherin expression have been correlated with histopathology and differentiation, but results are still inconsistent. Further studies should aid the development of novel strategies for both prevention and treatment of cancer. Aberrant methylation in promoter regions of the genes might lead to reduce gene expression and result in cancer. Thus in the present study for better understanding role of the E- cadherin in CRC, methylation status of the CDH1 promoter region was determined by methylation specific PCR following bisulphite modification, and compared with mRNA E-cadherin expression by qPCR. 43 of 70 cancers (61.4%) were aberrant methylated in the CDH1 promoter region and this correlated with reduced E-cadherin expression (p<0.05). Generally, our study demonstrated aberrant methylation of CDH1 can significantly reduce its expression among tumor tissues in CRC, and contribute to tumor staging, tumor differentiation and progression or prognosis.