Microbial load on raw bee pollen and bee bread across mid- and high-elevation areas in Bukidnon, Philippines

Paper Details

Research Paper 17/04/2023
Views (268) Download (39)
current_issue_feature_image
publication_file

Microbial load on raw bee pollen and bee bread across mid- and high-elevation areas in Bukidnon, Philippines

Carolina D. Amper, Myrna G. Ballentes, Elviro A. Garcines Jr.
J. Bio. Env. Sci.22( 4), 63-68, April 2023.
Certificate: JBES 2023 [Generate Certificate]

Abstract

The bacterial population on raw bee pollen and bee bread did not vary significantly across the mid- and high-elevation areas planted with Arabica coffee, with values that range from 5.4 x 106 to 6.4 x 106cfu/g. On the other hand, significant and highly significant differences in the fungal population across the three locations were observed on bee pollen and bee bread, respectively. A low fungal population was recorded on bee pollen collected from the high-elevation area (Site 3-Miarayon, Talakag) with 3.6 x 104cfu/g which was comparable to the population on samples collected from the mid-elevation area (Site 1-Imbayao, Malaybalay City) with 5.4 x 104cfu/g. Similarly, a low fungal count was recorded on bee bread collected from the high-elevation area (Site 3-Miarayon, Talakag) with 3.0 x 104cfu/g. Moreover, seven bacterial isolates were associated with the bee pollen and bee bread samples; three colonies were Gram-positive and four colonies were Gram-negative based on their reaction to Gram stain. On the other hand, yeasts and filamentous fungal species (Aspergillus, Fusarium, and Penicillium) were present in the two honey bee products. This study demonstrates that the fungal population in raw samples of bee pollen and bee bread from Arabica coffee plants grown at high elevation is lower than that in samples from mid-elevation areas.

VIEWS 75

Asama T, Arima TH, Gomi T, Keishi T, Tani H, Kimura Y, Tatefuji T, Hashimoto K. 2015. Lactobacillus kunkeei YB38 from honeybee products enhances IgA production in healthy adults. Journal of Applied Microbiology 119, 818–826. https:// sfamjournals. onlinelibrary.wiley.com /doi/ 10.1111

Corby-Harris V, Maes P, Anderson KE. 2014. The bacterial communities associated with honey bee (Apis mellifera) foragers. PloS ONE 9(4), e95056. https://doi.org/10.1371 /journal.pone.0095056

Cridge AG, Leask MP, Duncan EJ, Dearden PK. 2015. What do studies of insect polyphenisms tell us about nutritionally-triggered epigenomic changes and their consequences? Nutrients 7(3), 1787-1797. https://doi.org/10.3390/nu7031787

Donkersley P, Rhodes G, Pickup R, Jones K, Wilson K. 2018. Bacterial communities associated with honeybee food stores are correlated with land use. Ecology and Evolution 8, 4743–4756. https:// www.ncbi.nlm.nih.gov/pmc/articles/PMC5980251

Fratini F, Turchi B, Gasperini M, Torracca B, Giusti M, Sagona S, Felicioli A, Cerri D. 2014. Bee-gathered pollen loads suspension: preliminary assessment of interaction with microbial growth for a potential employment as a natural food additive. Journal of Microbiology, Biotechnology and Food Sciences 3(6), 467-469. https://arpi.unipi.it /retrieve /handle/11568/454067/236707/2014%20Fratini%20et%20al%20%20pollen%20as%20additive.pdf

Friedle C, D’ Alvise P, Schweikert K, Wallner K, Hasselmann M. 2021. Changes of microorganism composition in fresh and stored bee pollen from Southern Germany. Environmental Science and Pollution Research 28, 47251-47261. https://doi.org/10.1007/s11356-021-13932-4

González G, Hinojo MJ, Mateo R, Medina A, Jiménez M. 2005. Occurrence of mycotoxin producing fungi in bee pollen. International Journal of Food Microbiology 105(1), 1-9. https://pubmed .ncbi.nlm.nih.gov/16009441/

Hani B, Dalila B, Saliha D, Daoud H, Mouloud G, Seddik K. 2012. Microbiological sanitary aspects of pollen. Advance Environmental Biology 6, 1415-1420. https://www.researchgate.net/publication /286758280_Microbiological_sanitary_aspects_of_pollen/link/56dd2fd608aebabdb415abc7/download

Nainu F, Masyita A, Bahar MA, Raihan M, Prova SR, Mitra S, Emran TB, Simal-Gandara J. 2021. Pharmaceutical prospects of bee products: special focus on anticancer, antibacterial, antiviral, and antiparasitic properties. Antibiotics 10, 822. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300842/pdf/antibiotics-10-00822.pdf

Nogueira C, Iglesias A, Feás X, Estevinho LM. 2012. Commercial bee pollen with different geographical origins: a comprehensive approach. International Journal of Molecular Science 13(9), 11173-11187. https://doi.org/10.3390/ ijms130911173

Pełka K, Worobo RW, Walkusz J, Szweda P. 2021. Bee pollen and bee bread as a source of bacteria producing antimicrobials. Antibiotics 10, 713. https://doi.org/ 10.3390/antibiotics10060713