Microbial load on raw bee pollen and bee bread across mid- and high-elevation areas in Bukidnon, Philippines

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Research Paper 17/04/2023
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Microbial load on raw bee pollen and bee bread across mid- and high-elevation areas in Bukidnon, Philippines

Carolina D. Amper, Myrna G. Ballentes, Elviro A. Garcines Jr.
J. Bio. Env. Sci.22( 4), 63-68, April 2023.
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The bacterial population on raw bee pollen and bee bread did not vary significantly across the mid- and high-elevation areas planted with Arabica coffee, with values that range from 5.4 x 106 to 6.4 x 106cfu/g. On the other hand, significant and highly significant differences in the fungal population across the three locations were observed on bee pollen and bee bread, respectively. A low fungal population was recorded on bee pollen collected from the high-elevation area (Site 3-Miarayon, Talakag) with 3.6 x 104cfu/g which was comparable to the population on samples collected from the mid-elevation area (Site 1-Imbayao, Malaybalay City) with 5.4 x 104cfu/g. Similarly, a low fungal count was recorded on bee bread collected from the high-elevation area (Site 3-Miarayon, Talakag) with 3.0 x 104cfu/g. Moreover, seven bacterial isolates were associated with the bee pollen and bee bread samples; three colonies were Gram-positive and four colonies were Gram-negative based on their reaction to Gram stain. On the other hand, yeasts and filamentous fungal species (Aspergillus, Fusarium, and Penicillium) were present in the two honey bee products. This study demonstrates that the fungal population in raw samples of bee pollen and bee bread from Arabica coffee plants grown at high elevation is lower than that in samples from mid-elevation areas.


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