Micropropagation of mint (Mentha spicata)
Paper Details
Micropropagation of mint (Mentha spicata)
Abstract
Mentha spicata is a valuable, medicinally important, economic, essential oil-yielding perennial herb that is grown worldwide both in cultivated and wild forms. The present experiment was conducted at Biotechnology Laboratory in the Department of Biotechnology, Sher-e-Bangla Agricultural University to evaluate the effect of different concentrations of Benzyl adenine (BA) (1.0, 1.5, 2.0 and 2.5 mg/l) and Indole-3-butyric acid (IBA) (0.5, 1.0, 1.5 and 2.0 mg/l) either alone or in combination on micropropagation of mint. The treatment of 2.0 mg/L BA performed best in respect of percent response of explants (80.00%), number of shoots per explant (19.75) and shoot length (12.12 cm). In contrast, the maximum shoot number per explant (20.33) and shoot length (13.0 cm) was found in 2.0 mg/L BAP+1.0 mg/L IBA treatment. The maximum number of roots (3.4 and 5.2) and root length (7.50 and 7.67 cm) was observed in 1.0 mg/L BA and 2.0 mg/L BA in combination with 1.5 mg/L of IBA. Survival rate of regenerated plantlets 80 % in open atmosphere. Finally, feasible micropropagation protocol of mint has been developed that can be used for further improvement programme of breeding.
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