home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Micropropagation of mint (Mentha spicata)

Paper Details

Research Paper 05/08/2024
Views (107) Download (18)
current_issue_feature_image
publication_file

Micropropagation of mint (Mentha spicata)

Sayed Shaqur Ahmed, Homayra Huq, Farhana Afrin Vabna, Fahima Khatun
Int. J. Biosci.25( 2), 97-102, August 2024.
Certificate: IJB 2024 [Generate Certificate]
Key: BAIBAIn vitroMentha spicataRegeneration

Abstract

Mentha spicata is a valuable, medicinally important, economic, essential oil-yielding perennial herb that is grown worldwide both in cultivated and wild forms. The present experiment was conducted at Biotechnology Laboratory in the Department of Biotechnology, Sher-e-Bangla Agricultural University to evaluate the effect of different concentrations of Benzyl adenine (BA) (1.0, 1.5, 2.0 and 2.5 mg/l) and Indole-3-butyric acid (IBA) (0.5, 1.0, 1.5 and 2.0 mg/l) either alone or in combination on micropropagation of mint. The treatment of 2.0 mg/L BA performed best in respect of percent response of explants (80.00%), number of shoots per explant (19.75) and shoot length (12.12 cm).  In contrast, the maximum shoot number per explant (20.33) and shoot length (13.0 cm) was found in 2.0 mg/L BAP+1.0 mg/L IBA treatment. The maximum number of roots (3.4 and 5.2) and root length (7.50 and 7.67 cm) was observed in 1.0 mg/L BA and 2.0 mg/L BA in combination with 1.5 mg/L of IBA. Survival rate of regenerated plantlets 80 % in open atmosphere. Finally, feasible micropropagation protocol of mint has been developed that can be used for further improvement programme of breeding.

VIEWS 52
Cover PDF

Akter KT, Hoque MA. 2018. In Vitro Shoot Regeneration of Mint (Mentha Sp. L.) Using Different Types of Explants and Levels of Benzyl aminopurine. Bangladesh Journal of Agricultural Research 43, 703–716.

Bariya RR, Pandya HA. 2014. Evaluation and establishment of promising largescale in vitro production of corn mint. International Journal of Recent Scientific Research 5(2), 509-512.

Chaturvedi HC, Jain M, Kidwai R. 2007. Cloning of medicinal plants through tissue culture- A review. Indian Journal of Experimental Biology 45, 937-948.

Kane M. 2014. Plant Micropropagation. Environmental Horticulture Department: University of Florida. USA.

Liu BL, Fan ZB, Liu ZQ, Qiu XH, Jiang YH. (2018). Comparison of phytochemical and antioxidant activities in micropropagated and seed-derived Salvia miltiorrhiza plants. Hort Science 53(7), 1038-1044.

Lyczko J, Piotrowski K, Kolasa K, Galek R, Szumny A. 2020. Mentha piperita L. Micropropagation and the Potential Influence of Plant Growth Regulators on Volatile Organic Compound Composition. Molecules 25, 2652.

Mehta J, Naruka R, Sain M, Dwivedi A, Sharma D. 2012. An efficient protocol for clonal micropropagation of Mentha piperita L. (Peppermint). Asian Journal of Plant Science and Research 2(4), 518–523.

Najafianashrafi E. 2021. Metabolic engineering of essential oil composition in Lavendula latifolia by altering expression of borneol diphosphate synthase (Doctoral dissertation, University of British Columbia) 1-136.

Pati PK, Rath SP, Sharma M, Sood A, Ahuja PS. 2006. In vitro propagation of rose-a review. Biotechnology Advances 24(1), 94–114.

Safaeikhorram MS, Jafarneha S, Khosroshahi S. 2008. The world’s most important medicinal plants. Compilation Eric von Ben Vick, Michael Vynk. Iran Releases Green Farm Training Complex: 422.

Sharma MM, M Dhingra, A Dave, A Batra. 2012. Plant regeneration and stimulation of in vitro flowering in Eruca sativa Mill. African Journal of Biotechnology 11(31), 7906-7911.

Trevisan SCC, Menezes APP, Barbalho SM, Guiguer EL. 2017. Properties of Mentha piperita: a brief review. World Journal of Pharmaceutical and Medical Research 3(1), 309–313.

Zayova E, Kirova E, Geneva M. 2021. Optimized cultural conditions for rapid in vitro propagation and conservation of Mentha piperita L. Comptes rendus de l’Académiebulgare des Sciences 74, 945-54.

Zhao H, Shan R, Han Y, Shaun T, Chenyang G,  Maolun L. 2022. Peppermint Essential oil; its phytochemistry, biological activity, pharmacological effect and application. Biomedicine & Pharmacotherapy 154.

Submit Manuscript