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Micropropagation of tuna (Opuntia ficus – indica ) and effect of medium composition on proliferation and rooting

Akram Ghaffari, Tahereh Hasanloo, Mojtaba Khayam Nekouei

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Int. J. Biosci.3(11), 129-139-, November 2013

DOI: http://dx.doi.org/10.12692/ijb/3.11.129-139


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The goal of this study was to determine micropropagation system for a mass production of Tuna (Opuntia ficus – indica). For this reason, explants dissected from strilled young cladodes successfully established on Murashige and Skoog (MS) medium supplemented with 5 mg l-1 Benzyl amino purine (BAP). MS medium containing different combinations of BAP (5 mg l-1) and Indole acetic acid (IAA) (0, 0.25, 0.5, 1, 2 mg l-1) , BAP (5 mg l-1) and Naphtalene acetic acid (NAA) ( 0, 0.25, 0.5, 1, 2 mg l-1) and BAP (0.5 and 1 mg l-1) and Kinetin ( 0.5 and 1 mg l-1) were tested for shoot development . The best results for shoot development and elongation were obtained in media containing 5 mg l-1 and 0.25 mg l-1 NAA. The highest multiplication rate (3.9) was observed in media supplemented with 5 mg l-1 BAP and 2 mg l-1 of NAA. Satisfactory rooting was achieved in MS Basal medium (5-6 cm length) without callus formation. The percentage of rooting was 100% and Plants were successfully established in a mix of pit and perlite (2:1) (100%) and acclimatization accomplished under greenhouse condition. In this study, total concentration of carbohydrates and proteins were measured in in vivo cultured (control) and in vitro propagated tuna after 3 months. As a result, no significant differences were observed between control and micropropagated tuna in protein concentration. Whereas carbohydrate content in micropropagated plants (3.24 mg g-1) was 2- fold that of the control plants (1.52 mg g-1).


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Micropropagation of tuna (Opuntia ficus – indica ) and effect of medium composition on proliferation and rooting

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