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Micropropagation of tuna (Opuntia ficus – indica ) and effect of medium composition on proliferation and rooting

Akram Ghaffari, Tahereh Hasanloo, Mojtaba Khayam Nekouei

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Int. J. Biosci.3(11), 129-139-, November 2013

DOI: http://dx.doi.org/10.12692/ijb/3.11.129-139

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Abstract

The goal of this study was to determine micropropagation system for a mass production of Tuna (Opuntia ficus – indica). For this reason, explants dissected from strilled young cladodes successfully established on Murashige and Skoog (MS) medium supplemented with 5 mg l-1 Benzyl amino purine (BAP). MS medium containing different combinations of BAP (5 mg l-1) and Indole acetic acid (IAA) (0, 0.25, 0.5, 1, 2 mg l-1) , BAP (5 mg l-1) and Naphtalene acetic acid (NAA) ( 0, 0.25, 0.5, 1, 2 mg l-1) and BAP (0.5 and 1 mg l-1) and Kinetin ( 0.5 and 1 mg l-1) were tested for shoot development . The best results for shoot development and elongation were obtained in media containing 5 mg l-1 and 0.25 mg l-1 NAA. The highest multiplication rate (3.9) was observed in media supplemented with 5 mg l-1 BAP and 2 mg l-1 of NAA. Satisfactory rooting was achieved in MS Basal medium (5-6 cm length) without callus formation. The percentage of rooting was 100% and Plants were successfully established in a mix of pit and perlite (2:1) (100%) and acclimatization accomplished under greenhouse condition. In this study, total concentration of carbohydrates and proteins were measured in in vivo cultured (control) and in vitro propagated tuna after 3 months. As a result, no significant differences were observed between control and micropropagated tuna in protein concentration. Whereas carbohydrate content in micropropagated plants (3.24 mg g-1) was 2- fold that of the control plants (1.52 mg g-1).

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Micropropagation of tuna (Opuntia ficus – indica ) and effect of medium composition on proliferation and rooting

Aliyu B, Mustapha Y. 2007. Effect of different media on the in vitro growth of cactus (Opuntia ficus-indica) explants. African Journal of Biotechnology 6, 1330-1331.

Ault JR, Blackmon WJ. 1987. In vitro propagation of Ferocactus acanthodes (Cactaceae). HortScience 22, 126–127.

Barrientos PF, Brauer O. 1964. Multiplicacio´n vegetativa Del nopal a partir de fracciones mı´nimas. Colegio de Postgraduados, E.N.A. Chapingo, Me´xico.

Bein E. 1996. Useful trees and shrubs in Eritrea. Regional Soil Conservation Unit (RSCU), Nairobi, Kenya.

Clayton PW, Hubstenberger JF, Phillips GC. 1990. Micropropagation of members of the cactaceae subtribe cactinae. Journal of the American Society for Horticultural Science 115(2), 337–343.

Escobar-Araya HA, Villalobos AVM, Villegas MA. 1986. Opuntia micropropagation by axillary proliferation. Plant Cell, Tissue and Organ Culture 7, 269–277. http://dx.doi.org/10.1007/BF00037744

Estrada-Luna AA, Martınez-Hernandez JJ, Torres-Torres ME, Chable´-Moreno F. 2008. In vitro micropropagation of the ornamental prickly pear cactus Opuntia lanigera Salm–Dyck and effects of sprayed GA3 after transplantation to ex vitro conditions. Scientia Horticulturae 117, 378–385. http://dx.doi.org/10.1016/j.scienta.2008.05.042

Estrada-Luna AA. 1988. Produccio´n de brotes e injertacio´n in vitro de seis species de nopal (Opuntia spp.) originarias del Altiplano Potosino-Zacatecano. Tesis de MC. Colegio de Postgraduados, Montecillo, Edo, De Me´ xico, Me´ xico, 160.

Estrada-Luna AA, Davies FT. 2001. Mycorrhizal fungi enhance growth and nutrient uptake of prickly-pear cactus (Opuntia albicarpa Scheinvar ‘‘Reyna’’) plantlets after ex vitro transplantation. Journal of Horticultural Science & Biochemistry 76 (6), 739– 745.

Estrada-Luna AA, Lo´pez-Peralta C, Ca´rdenas-Soriano E. 2002. In vitro micrografting and histology of the graft union formation of selected species of prickly pear cactus (Opuntia spp.). Scientia Horticulturae 92, 317–327. http://dx.doi.org/10.1016/S0304-4238(01)00296-5

Fabbri A, Cicala A, Tamburino A. 1996. Anatomy of adventitious root formation in Opuntia ficus-indica cladodes. Journal of Horticultural Science 71(2), 235–242.

Fay MF, Gratton J, Atkinson J. 1995. Tissue culture of succulent plants—an annotated bibliography. Bradleya 13, 38–42.

Flores-Valde´z CA. 1995. ‘‘Nopalitos’’ production, processing and marketing. In: Barbera G, Inglese P, Pimienta-Barrios E, ed. Agroecology, Cultivation and Uses of Cactus Pear, FAO, Rome, 92–99.

Garcı´a-Aguilar M, Pimienta-Barrios E. 1996. Cytological evidences of agamospermy in Opuntia (Cactaceae). Haseltonia (Iowa) 4, 39–42.

Garcı´a-Saucedo P, Valdez-Morales M, Valverde ME, Cruz-Herna´ndez A, Paredes-Lo´pez O. 2005. Plant regeneration of three Opuntia genotypes used as human food. Plant Cell, Tissue and Organ Culture 80, 215–219. http://dx.doi.org/10.1007/s11240-004-9158-0

Hartmann HT, Kester DE, Davies Jr FT, Geneve RL. 1997. Plant Propagation- Principles and Practices, 6th ed. Prentice-Hall, Englewood Cliffs, NJ, USA.

Havel L, Kolar Z. 1983. Microexplant isolation from Cactaceae. Plant Cell, Tissue and Organ Culture 2, 349–353. http://dx.doi.org/10.1007/BF00039882

Hegwood DA. 1990. Human health discoveries with Opuntia sp. (prickly pear). HortScience 25 (12), 1515–1516.

Infante R. 1992. In vitro axillary shoot proliferation and somatic embryogenesis of yellow pitaya Mediocactus coccineus (Salm-Dyck). Plant Cell, Tissue and Organ Culture 31, 155-159. http://dx.doi.org/10.1007/BF00037700

Irish M. 2001. The Ornamental Prickly Pear Industry in the Southwestern United States. Florida Entomologists 84 (4), 484–485.

Jacobs WP, Morrow IB. 1958. Quantitative relations between stages of leaf development and differentiation of sieve-tubes. Science, N.Y. 128, 1084-1085.

Johnson JL, Emino ER. 1979. Tissue culture propagation in the cactaceae. Cactus & Succulent Journal (U.S.) 51, 275–277.

Jua´rez MA, Passera CB. 2002. In vitro propagation of Opuntia ellisiana Griff. And acclimatizationto field conditions. Biocell 26(3), 319–324.

Lazcano CA, Davies Jr FT, Estrada-Luna AA, Duray SA, Olalde-Portugal V. 1999. Effect of auxin and wounding on adventitious root formation of pricklypear cactus cladodes. HortTechnology 9 (1), 99–102.

Le Houérou HN. 1994. Drought-tolerant and water-efficient fodder shrubs (DTFS), their role as a “drought insurance” in the agricultural Development of Arid and Semi-arid Zones in Southern Africa. Report to the Water Research Commission of South Africa.

Llamoca-Za´rate RM, Aguiar LF, Landsmann J, Campos FAP. 1999a. Whole plant regeneration from the shoot apical meristem of Opuntia ficus– indica Mill. (Cactaceae). Journal of Applied Botany 73, 83–85.

Llamoca-Za´ rate RM, Aguiar LF, Landsmann J, Campos FAP. 1999b. Establishment of callus and cell suspension cultures of Opuntia ficus-indica. Plant Cell, Tissue and Organ Culture 58, 155–157. http://dx.doi.org/10.1023/A:1006315729266

Mackay WA, Tipton JL, Thompson GA. 1995. Micropropagation of Mexican redbud, Cercis canadensis var. Mexicana. Plant Cell, Tissue and Organ Culture 43, 295-299. http://dx.doi.org/10.1007/BF00039959

Malda G, Suza´n H, Backhaus R. 1999. In vitro culture as potential method for the conservation of endangered plants possessing crassulacean acid metabolism. Scientia Horticulturae 81, 71–87. http://dx.doi.org/10.1016/S0304-4238(98)00250-7

Martinez-V~izquez O, Rubluo A. 1989. In vitro mass propagation of the nearextinct Mammillaria san-angelensis S~nchez-Mejorada. Journal of horticultural science 64, 99-105.

Mauseth JD. 1977. Cactus tissue culture: a potential method of propagation. Cactus & Succulent Journal 49(2), 80–81.

Mauseth JD. 1977. A new method for the propagation of cacti: sterile culture of axillary buds. Cactus & Succulent Journal 51, 186–187.

Mauseth JD, Halperin W. 1975. Hormonal control of organogenesis in Opuntia polyacantha (Cactaceae). American Journal of Botany 62(8), 869–877.

Mohamed-Yassen Y, Barringer SA, Splittstoesser WE, Schnell R. 1995. Rapid propagation of tuna (Opuntia ficusindica) and plant establishment in soil. Plant Cell, Tissue and Organ Culture 42, 117–119. http://dx.doi.org/10.1007/BF00037690

Mondrago´n, JC. 2001. Verification of the apomictic origin of cactus pear (Opuntia spp. Cactaceae) seedling of open pollinated and cross from Central Mexico. Journal of the Professional Association for Cactus Development 4, 49–56.

Mulas M, Spano S, Pellizaro G, D´ jallewin G. 1992. Rooting of Opuntia ficus-indica Mill. Young cladodes. Advanced Horticultural Science 6, 44–46. http://dx.doi.org/10.1400/14155

Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco cultures. Physiology Plantarum 15, 473–497. http://dx.doi.org/10.1111/j.13993054.1962.tb08052.x

Nishimura T, Hitoshi K, Furumai T, Igarashi Y, Sato Y, Shimizu M. 2003. Plant disease control agent. US Patent 6(544), 511B2.

Nobel PS. 1995. Environmental Biology. In: Agro-ecology, Cultivation and Uses of Cactus Pear. FAO Plant Production and Protection Paper, FAO, Rome, 36-48.

Nobel  PS.  1994.  Remarkable  Agaves  and  Cacti. Oxford University Press, New York, 86-87.

Pe´rez-Molphe-Balch E, Da´vila-Figueroa CA. 2002. In vitro propagation of Pelecyphora aselliformis Ehrenberg and P. strobiliformis Werdermann (Cactaceae). In Vitro Cellular and Developmental Biology Plant 38, 73–78. http://dx.doi.org/10.1079/IVP2001248

Pe´rez-Molphe-Balch E, Pérez-Reyes ME, Villalobos-Amador E, Meza-Rangel E, Morones-Ruiz LR, Lizalde-Viramontes H. 1998. Micropropagation of 21 species of Mexican Cacti by axillary proliferation. In Vitro Cellular & Developmental Biology – Plant 34, 131–135. http://dx.doi.org/10.1007/BF02822777

Rojas-Are´ chiga M, Va´squez-Yanes C. 2000. Cactus seed germination: a review. Journal of Arid Environments 44, 85–104. http://dx.doi.org/10.1006/jare.1999.0582

Sa´enz-Herna´ndez C. 1995. Food manufacture and by-products. In: Barbera G, Inglese P, Pimienta-Barrios E, ed. Agroecology, Cultivation and Uses of Cactus Pear. FAO, Rome, 137–143.

Sachar RC, Iyer RD. 1959. Effect of auxin, kinetin and gibberellin on the placental tissue of Opuntia dellenii Haw. Cultured in vitro. Phytomorphology 9, 1–3.

Saeidi M. 2008. Physiological and biochemical study of some aspects of the source and sink strength in both sensitive and tolerant cultivars under drought stress. PhD Thesis, Tehran University, Tehran, Iran, 67-70.

Silos-Espino H, Valde´z-Ortı´z A, Rasco´n-Cruz Q, Rodrı´guez-Salazar E, Paredes-Lo´pez O. 2006. Genetic transformation of prickly-pear cactus (Opuntia ficus indica) by Agrobacterium tumefaciens. Plant Cell, Tissue and Organ Culture 86, 397–403. http://dx.doi.org/10.1007/s11240-006-9123-1

Taiz L, Zieger E. 1998. Plant Physiology, 2nd ed. Sinauer Associates Inc., Massachusetts.

Torrey JG. 1963. Cellular patterns in developing roots. Symposia of the Society for Experimental Biology 17, 285-314.

Vanegas-Espinoza PE, Cruz–Herna´ndez A, Valverde ME, Paredes-Lo´pez O. 2002. Plant regeneration via organogenesis in marigold. Plant Cell, Tissue and Organ Culture 3, 279–283. http://dx.doi.org/10.1007/BF00039959

Ve´lez GC, Rodrı´guez GB. 1996. Microscopic analysis of poliembriony in Opuntia ficus-indica. Journal  of  the  Professional  Association  for  Cactus Development 1, 39–48.

Wetmore RH, Demaggio AE, Rier JP. 1964. Contemporary  outlook  on  the  differentiation  of vascular tissues. Phytomorphology 14, 203—217.

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