Int. J. Agron. Agri. Res.5(2), 55-63, August 2014
Microsatellite markers were used to characterize the millennium olive cultivars localized in nine different archeological sites in Tunisia. Thirty genotypes were considered for genetic fingerprinting using 10 pairs of microsatellite primers. The number of alleles per locus ranged from 3 to 5, with a mean of 3.7 alleles per primer pair (a total of 37 alleles). The observed heterozygosity ranged from 0.4 to 1, while the expected heterozygosity varied between 0.37 and 0.74. The polymorphism information content values ranged also from 0.37 to 0.74. The mean polymorphism information content value of 0.61 for the SSR loci provided sufficient discriminating ability to evaluate the genetic diversity among the millennium cultivars. The UPGMA cluster analyses using Jaccard’s index permitted a segregation of the thirty millennium cultivars in three main groups and revealed that most of the millennium cultivars grouped according morphological parameters of the fruit and the endocarp and no clear clustering trends were observed according to their geographic origin. As a sequel to the present work, new surveys should be made in the archeological sites localized in North and the Center of Tunisia to sample more cultivars and to draw a clearer picture of the diversity of the Tunisian millennium olive germplasm.
Abdelhamid S, Kamoun G, Francesco M, Tiziano C. 2012. Genetic similarity among Tunisian cultivated olive estimated through SSR markers. Scientia Agricola.70 (1), 33-38.
Angiolillo A, Mencuccini M, Baldoni L. 1999. Olive genetic diversity assessed using amplified fragment length polymorphism. Theoretical and Applied Genetics 98, 411–421.
Baldoni L, Cultrera NG, Mariotti R, Ricciolini C. 2009. A consensus list of microsatellite markers for olive genotyping. Molecular Breeding. 24, 213-231.
Barranco D and Rallo L. 2000. Olive cultivars in Spain. Hortechnology 10,107-110.
Belaj A, Trujillo I, De La Rosa R, Rallo L. 2001. Polymorphism and discriminating capacity of randomly amplified polymorphic markers in an olive germplasm bank. of the American Society for Horticultural Science. 126, 64-71.
Belaj A, Muñoz-Diez C, Baldoni L and Satovic Z. 2010. Genetic diversity and relationships of wild and cultivated olives at regional level in Spain. Scientia Horticulturae.124, 323-330.
Besnard G, Baradat P, Bervillé A. 2001. Genetic relationships in the olive (Olea europaea L.) reflect multilocal selection of cultivars. Theoretical and Applied Genetics.102, 251-258.
Botstein D, White RL, Skolnick M and Davis RW.1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal in Human Genetics 32, 314-331.
Bracci T, Busconi M, Fogher C, Sebastiani L.2011. Molecular studies in olive (Olea europaea L.): overview on DNA markers applications and recent advances in genome analysis. Plant Cell Reports. 30, 449-462.
Brown JP. 2004. Wine and oil in the antique Mediterranean. Publishing «Saint- Etienne » house, France.
Camps-Fabrer H. 1997. The olive cultivation of olive in the North Africa, Evolution and history, in: International Olive Oil Council (Eds), World Encyclopaedia of Olive. Madrid, Espagne.
Carriero F, Fontanazza G, Cellini F, Giorio G. 2002. Identification of simple sequence repeats (SSRs) in olive (Olea europaea L.). Theoretical and Applied Genetics 104, 301–307.
Chevalier A. 1948. Origine of olive cultivars. Revue de botanique appliquée et d’agriculture tropicale 28, 1–25.
Cifferi R. 1950. Primary elements for the study of the origin and the evolution of the cultivated olive. Acte du XIII congres International d’oléiculture 1, 189–194.
Cipriani G, Marrazzo M T, Marconi R, Cimato A, Testolin R. 2002. Microsatellite markers isolated in olive (Olea europaea L.) are suitable for individual fingerprinting and reveal polymorphism within ancient cultivars. Theoretical and Applied Genetics 104, 223–228.
De La Rosa R, James C, Tobutt KR. 2002. Isolation and characterization of polymorphic microsatellite in olive (Olea europaea L.) and their transferability to other genera in the Oleacea. Molecular Ecology.2, 265-267.
Delgado-Martinez FJ, Amaya I, Sánchez-Sevilla JF, Gomez-Jimenez MC. 2012. Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain. Genetic Molecular. Research. 11 (2) 918-932.
Diaz A, De La Rosa R, Martin A, Rallo P.2006. Development, characterization and inheritance of new microsatellites in olive (Olea europaea L.) and evaluation of their usefulness in cultivar identification and genetic relationship studies. Tree Genet. Genomes. 2, 165-175.
Dice LR. 1945. Measures of the amount of ecologic association between species. Ecology 26, 297-302.
Doveri S, Sabino Gil F, Diaz A, Reale S. 2008. Standardization of a set of microsatellite markers for use in cultivar identification studies in olive (Olea europaea L.). Scientia Horticulturae.116, 367-373.
Fabbri A, Hormaza JI, Polito VS. 1995. Random amplified polymorphic DNA analysis of olive (Olea europaea L.).Journal of the American Society for Horticultural Science 120, 538–542.
Gemas VJV, Rijo-Johansen MJ, Tenreiro R, Fevereiro P. 2000. Inter-and Intra-varietal analysis of three Olea europaea L. cultivars using the RAPD techniques. Journal of Horticulture Science and Biotechnology. 75,312–319.
Guerin RJ, Sweeney SM, Collins GG, Sedley M. 2002. The development of a genetic database to identify olive cultivars. Journal of the American Society for Horticultural Science. 127,977–983.
Jaccard P. 1901. Comparative study of floral distribuition in the mountains of the Alps and the Jura, Bulletin Societé Vandoise des Sciences Naturelles 37, 547-579.
Kamoun Garrati N, Lamy FM, Rebaî A, Gargouri A, Panaud O, Saar A. 2006. Genetic diversity of Tunisian olive tree (Olea europaea L.) cultivars assessed by AFLP markers. Genetic Resources and Crop Evolution 53, 265–275.
Loukas M, Krimbas C B. 1983. History of olive cultivars based on their genetic distances. Journal of Horticulture Sciences 58, 121–127.
Loussert L, Brousse G. 1978. Olive tree: Mediterranean Agricultural Techniques of olive production. Publishing « neuve and Larose » house, 44-111.
Mekuria GT, Collins GG, Sedgley M.1999. Genetic variability between different accession of some common commercial olive cultivars. Journal of Horticulture Science and Biotechnology.74,309–314.
Mnasri Rahmani S, Saddoud Dabbebi O, Ferchichi A. 2013. Preliminary characterization and morph-agronomic evaluation of millennium olive varieties in Tunisia. Journal of Biodiversity and Environmental Sciences 3 (8), 150-155.
Mnasri Rahmani S, Saddoud Dabbebi O, Ben Saleh M, Ferchichi A. 2014. DNA fingerprinting of millennium olive varieties in Tunisia by AFLP markers. Journal of Biodiversity and Environmental Sciences 4 (4), 310-317.
Nei, M. 1987. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89,583–590.
Nei M, Li WH. 1979. Mathematical model for studying genetic variation in terms of endonucleases. Proceedings of the National Academy Sciences of the United States of America 76, 5269–5273.
Ouazzani N, Lumaret R, Villemur P, Di Giusto F. 1993. Leaf allozyme variation in cultivated and wild olive trees (Olea europaea L.). Journal of Heredity 84, 34–42.
Reale S, Doveri S, Dıaz A, Angiolillo A, Lucentini L, Pilla F, Martin A, Donini P, Lee D. 2006. SNP-based markers for discriminating olive (Olea europaea L.) cultivars. Genome 49, 1193-1205.
Rekik I, Salimonti A, Innocenzo M, Oliver L, Sophie G. 2008. Characterization and Identification of Tunisian Olive Tree Varieties by Microsatellite Markers. HOrtscience. 43(5), 1371–1376.
Rohlf, M. 1998. NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System: Version 2.02. Exeter Software, Setauket, NY, USA.
Sanz-Cortez F, Badenes ML, Paz S, Iniguez A. 2001. Molecular characterization of olive cultivars using RAPD markers. Journal of the American Society for Horticultural Science.126, 7-12.
Sarri V, Baldoni L, Porceddu A, Cultrera NG. 2006. Microsatellite markers are powerful tools for discriminating among olive cultivars and assigning them to geographically defined populations. Genome. 49, 1606-1615.
Sefc K M, Lopes M S, Mendoncxa D, Rodrigues dos Santos M, Laimer da Camara Machado M, Da Camara Machado A. 2000. Identification of microsatellite loci in olive (Olea europea L.) and their characterization in Italian and Iberian olive trees. Molecular Ecology. 9,1171–1173.
Sensi E, Vignani R, Scali M, Masi E, Cresti M. 2003. DNA fingerprinting and genetic relatedness among cultivated varieties of Olea europea L. estimated by AFLP analysis. Scientia Horticulurae. 97,379–388.
Taamalli W, Geuna F, Banfi R, BassiD, Daoud D, Zarrouk M. 2006. Agronomic and molecular analyses for the characterisation of accessions in Tunisian olive germplasm collections. Electronic Journal of Biotechnology 9, 467–481.