Welcome to International Network for Natural Sciences | INNSpub

Mitigation of adverse effects of heat stress in chillies by using glycine betaine

Research Paper | August 1, 2019

| Download 15

Tanveer Hussain, Choudhary Muhammad Ayyub, Ijaz Ahmad, Irfan Ali, Zaid Mustfa, Adeel Anwar, Aqeel Ahmad, Sohail Latif, Tanveer Iqbal

Key Words:

Int. J. Biosci.15( 2), 1-10, August 2019

DOI: http://dx.doi.org/10.12692/ijb/15.2.1-10


IJB 2019 [Generate Certificate]


Chilli (Capsicum spp.) is an important vegetable cum spice crop of the night shade family requiring 20-30 °C optimum temperatures for plant growth and development, usually growth starts retarding below 15°C or above 32°C temperature. Almost all growth stages of chilli plants are influenced by high temperatures ultimately leads to economic yield losses in final crop productivity. The experiment was carried out in growth chamber of mushroom lab, Institute of Horticultural Sciences, University of Agriculture, Faisalabad aiming at identifying the best glycine betaine treatment proved to be useful in coping with adversaries of high temperature stress in chillies. Chilli genotypes named as C-37, Uk-101, H-13 and jawala were grown and sprayed with different concentrations (0, 5, 10, 15 and 20 mM) of glycine betaine at the seedling stage under high temperature stress (40/32ºC day and night temperature) in growth chamber provided with controlled conditions. Various physiological attributes of chilli genotypes were recorded. Glycine betaine application @15mM was best for enhancing the heat tolerance potential of chilli genotypes under heat stress. Glycine betaine has also been proved effective in enhancing the heat tolerance potential under high temperature stress.


Copyright © 2019
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Mitigation of adverse effects of heat stress in chillies by using glycine betaine

Afzal M, Ahmad A, Alderfasi AA, Ghoneim A, Saqib M. 2014. Physiological tolerance and cation accumulation of different genotypes of Capsicum annum under varying salinity stress, Proceedings of the International Academy of Ecology and Environmental Sciences 4(1), 39-49.

Akhund S, Akram A, Hanif NQ, Qureshi R, Naz F, Nayyar BG. 2017. Pre-harvest aflatoxins and Aspergillus flavus contamination in variable germplasms of red chillies from Kunri, Pakistan. Mycotoxin research 33(2), 147-155.

Alberto GZ, Campos ES, Ortega JG, Morales RP, Ortiz JCR, Hernández JL. 2013. Characterization of different capsicum varieties by evaluation of their capsaicinoids content by high performance liquid chromatography, determination of pungency and effect of high temperature. Molecular Journal 18, 13471-13486.

Ashraf M, Foolad MR. 2007.  Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental Experimental Botany 59, 206-216.

Bramley PM. 2000. Is lycopene beneficial to human health?. Phytochemistry 54(3), 233-236.

Carvalho JA, Rezende FC, Aquino RF, Freitas WA, Olivera CE. 2011. Productive and economic analysis of red-pepper under different irrigation depths cultivated in greenhouse. Rev Bras Eng Agrículture Ambient 15, 569-574.

Chen THH, Murata N. 2008. Glycine betaine: an effective protectant against abiotic stress in plants. Trends Plant Sciences 13, 499-505.

Chinnusamy V, Jagendorf A, Zhu JK. 2005. Understanding and improving salt tolerance in plants Journal of Crop Sciences 45, 437-448.

Delgado-Gayt MF, Rosas-Rodríguez JA, Yepiz-Plascencia G, Figueroa-Soto CG, Valenzuela-Soto EM. 2017. Cloning and molecular characterization of the betaine aldehyde dehydrogenase involved in the biosynthesis of glycine betaine in white shrimp (Litopenaeus vannamei). Chemico-Biological Interactions. P 1-10.

Farooq M, Aziz T, Hussain M, Rehman H, Jabran K, Khan MB. 2008. Glycine betaine improves chilling tolerance in hybrid maize. Journal of Agronomy and Crop Sciences 194, 152-160.

Genard H, LeSaos J, Hillard J, Tremolieres A, Boucaud J. 1991. Effect of salinity on lipid composition, glycine betaine content and photosynthetic activity in chloroplasts of Suaeda maritime. Plant Physiology and Biochemistry 29, 421-427.

Hall AE. 2001. Crop responses to environment. CRC Press LLC, Boca Raton, Florida.

Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ. 2000. Plant cellular and molecular responses to high salinity. Annu. Rev. Plant Physiol. Plant Molecular Biology 51, 463-499.

Holmberg N, Bulow L. 1998.  Improving stress tolerance in plant by gene transfer. Trends Plant Sciences 3, 61-66.

Howard LR, Talcott ST, Brenes CH, Villalon B. 2000. Changes in phytochemical and antioxidant activity of selected pepper cultivars (Capsicum species) as influenced by maturity. Journal of Agriculture and Food Chemistry 48(5), 1713-1720.

Huve K, Bichele I, Tobias M, Niinemets U. 2005. Heat sensitivity of photosynthetic electron transport varies during the day due to changes in sugars and osmotic potential. Plant Cell Environment. 29, 212-218.

IPCC. 2012. Managing the risks of extreme events and disasters to advance climate change adaptation. A special report of working groups I and II of the intergovernmental panel on climate change, C.B. Field, Cambridge University Press.

Kasuga M, Liu W, Miura S, Yamaguchi-Shinozaki K, Shinozaki K. 1999. Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nat. Biotechnology 17, 287-291.

Korkmaz A, Rauf S Koca F Degera O, Demirkıri AR. 2012. Alleviation of salt-induced adverse effects in pepper seedlings by seed application of glycine betaine. Scientia Horticulure. 148, 197-205.

Korkmaz A, Topal T, Tan DX, Reiter RJ. 2009. Role of melatonin in metabolic regulation. Reviews in endocrine and metabolic disorders 10(4), 261-270.

Mansour MMF. 2000. Nitrogen containing compounds and adaptation of plants to salinity stress. Biol. Plant. 43, 491-500.

Marti MC, Camejo D, Vallejo F, Romojaro F, Bacarizo S, Palma JM, Sevilla JMF, Jiménez A. 2011. Influence of fuit ripening stage and harvest period on the antioxidant content of sweet pepper cultivars. Plant Food Human Nutrition 66, 416-423.

Mohanty A, Kathuria H, Ferjani A, Sakamoto A, Mohanty A, Murata N, Tyagi AK. 2002. Transgenics of an elite indica rice variety Pusa Basmati 1 harbouring the codA gene are highly tolerant to salt stress. Theoretical and Applied Genetics 106, 51-57.

Park RJ, Jeknic Z, Chen THH. 2006. Exogenous application of glycine betaine increases chilling tolerance in tomato plants. Plant Cell Physiology 47, 706- 714.

PPCR. 2010. Pakistan planning commission report, 2010.

Prabhavathi V, Yadav JS, Kumar PA, Rajam MV. 2002. Abiotic stress tolerance in transgenic eggplant (Solanum melongena L.) by introduction of bactrial mannitol phospho dehydrogenase gene. Molecular Breeding 9, 137-147.

Rhodes D, Hanson AD. 1993. Quaternary ammonium and tertiary sulfonium compounds in higher-plants. Annu. Rev. Plant Physiology and Plant Molecular Biology 44, 357-384.

Rontein D, Dieuaide-Noubhani M, Dufourc EJ, Raymond P, Rolin D. 2002. The metabolic architecture of plant cells stability of central metabolism and flexibility of anabolic pathways during the growth cycle of tomato cells Journal Biochemistry. 277(46), 43948-43960.

Safina N, Anjum MA, Ahmad I. 2006. Growth of chilli (Capsicum annuum L.) F1 hybrid sky line-2 in response to different ages of transplants. Journal of Research Sciences 17(2), 91-95.

Sairam RK, Tyagi A. 2004. Physiology and molecular biology of salinity stress tolerance in plants. Current Sciences Bangladesh 86(3), 407-421.

Serrano R, Culianz-Macia FA, Moreno V. 1999. Genetic engineering of salt and drought tolerance with yeast regulatory genes. Scientia Horticulturae 78, 261-269.

Shaked R, Rosenfeld K, Pressman E. 2004. The effect of night temperature on carbohydrates metabolism in developing pollen grains of pepper in relation to their number and functioning. Scientia Horticulturae 102, 29-36.

Sigge GO, Hansmann CF, Joubert F. 2001. Effect of storage conditions, packaging material and metabisulphite treatment on the colour of dehydrated green bell peppers (Capsicum annuum L.). Journal of Food Quality 24, 205-218.

Subbarao GV, Wheeler RM, Levine LH, Stutte GW. 2001. Glycine betaine accumulation, ionic and water relations of red-beet at contrasting levels of sodium supply. Journal of Plant Physiology 158, 767-776.

Surapu V, Ediga A, Meriga B. 2014. Salicylic acid alleviates aluminum toxicity in tomato seedlings (Lycopersicum esculentum Mill.) through Activation of antioxidant defense system and proline biosynthesis. Advance in Bioscience Biotechnology 5, 777-789.

Tanou G, Filippou P, Belghazi M, Diamantidis G, Fotopoulos V,  Molassiotis A.  2012. Oxidative and nitrosative-based signaling and associated post-translational modifications orchestrate the acclimation of citrus plants to salinity stress. Plant Journal 72, 585-599.

Venkatesan A, Chellappan KP. 1998. Accumulation of proline and glycine betaine in ipomoea pescaprae induced by NaCl. Biological Plant 4, 271-276.

Yang G, Rhodes D, Joly RJ. 2003. Effects of high temperature on membrane stability and chlorophyll fluorescence in glycine betaine deficient and glycine bctaine containing maize lines. Australian Journal Plant Physiology 23, 437-443.

Yang X, Lu C. 2005. Photosynthesis is improved by exogenous glycine betaine in salt-stressed maize plants. Physiology Plant 124, 343-352.

Zhu JK. 2001. Plant salt tolerance. Trends Plant Sci. 2, 66-71.