Mitochondrial gene marker sequences reveal identities of gobies at Minalungao National Park, Nueva Ecija, Philippines

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Mitochondrial gene marker sequences reveal identities of gobies at Minalungao National Park, Nueva Ecija, Philippines

Khristina G. Judan Cruz, Melinda N. Reyes, Perry Ayn Mayson A. Maza, Revin Arsee P. Cardona, Wilson R. Jacinto
J. Bio. Env. Sci.13( 2), 258-264, August 2018.
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Gobiidae is a rapidly growing family besides being the largest, with new species continuously discovered in all parts of the world, specifically in Asia and in the Philippines.  Given this diversity, this study provides an initial identification and is the first report of gobies found at Minalungao National Park, a Key Biodiversity Area (KBA) in Central Luzon, Philippines. Goby species were identified molecularly using mitochondrial genes namely:  CO1 (Cytochrome Oxidase 1), Control Region or D-Loop, and 16s rRNA. Samples were collected along Minalungao River. DNA was extracted from muscle tissues and gene markers were amplified using polymerase chain reaction. Sequences were subjected to BLAST for species identification. Results showed CO1 sequences ranging from 712-719 bp; D-Loop at 781-841 bp; and 559-592 bp in 16srRNA. BLAST sequences revealed four species of gobies belonging to 2 genera namely Rhinogobius giurinus (99% maximum identity using 16srRNA, 95% with D-Loop), Rhinogobius brunneus (97% matched using CO1), Glossogobius bicirrhosus (82% matched with D-Loop), and Glossogobius giuris (with 87% CO1 match).


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